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Ultrarapid Sodium Dodecyl Sulfate Polyacrylamide Mini-Gel Electrophoresis

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Electrophoretic Separation of Proteins

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1855))

Abstract

We describe here an ultrafast method for electrophoresing proteins on SDS-PAGE. Previously we reported a method to complete SDS-PAGE and immunoblotting in an hour, including electrophoresing proteins at 70°C in 10 min. Here we show that we can electrophorese molecular weight standards and bovine serum albumin on a 4–20% gradient gel in well under 10 min using heated (44 °C) Laemmli running buffer and high voltage.

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References

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Author information

Authors and Affiliations

  1. Arthritis and Clinical Immunology Program, Oklahoma Medical Research Foundation, Oklahoma City, OK, USA

    Rohit Thomas & Biji T. Kurien

  2. Oklahoma School of Science and Mathematics, Oklahoma City, OK, USA

    Rohit Thomas

  3. Department of Medicine, University of Oklahoma Health Sciences Center, Oklahoma City, OK, USA

    Biji T. Kurien

  4. Department of Veterans Affairs Medical Center, Oklahoma City, OK, USA

    Biji T. Kurien

Authors
  1. Rohit Thomas
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  2. Biji T. Kurien
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Corresponding author

Correspondence to Biji T. Kurien .

Editor information

Editors and Affiliations

  1. Department of Medicine, University of Oklahoma Health Sciences Center, Oklahoma City, OK, USA

    Biji T. Kurien

  2. Department of Medicine, University of Oklahoma Health Sciences Center, Oklahoma City, OK, USA

    R. Hal Scofield

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Thomas, R., Kurien, B.T. (2019). Ultrarapid Sodium Dodecyl Sulfate Polyacrylamide Mini-Gel Electrophoresis. In: Kurien, B., Scofield, R. (eds) Electrophoretic Separation of Proteins. Methods in Molecular Biology, vol 1855. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8793-1_42

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  • DOI: https://doi.org/10.1007/978-1-4939-8793-1_42

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8792-4

  • Online ISBN: 978-1-4939-8793-1

  • eBook Packages: Springer Protocols

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