Abstract
The migrasome is a newly discovered, migration-dependent membrane-bound cellular organelle. It functions in the active release of intracellular contents into the external environment and in cell–cell communications. Migrasomes have characteristic morphological features compared with intracellular organelles and extracellular vesicles. This unit describes methods for visualizing migrasomes by fluorescence microscopy and electron microscopy.
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References
Ma L et al (2015) Discovery of the migrasome, an organelle mediating release of cytoplasmic contents during cell migration. Cell Res 25:24–38
da Rocha-Azevedo B, Schmid SL (2015) Migrasomes: a new organelle of migrating cells. Cell Res 25:1–2
Trams EG et al (1981) Exfoliation of membrane ecto-enzymes in the form of micro-vesicles. Biochim Biophys Acta 645:63–70
Johnstone RM et al (1987) Vesicle formation during reticulocyte maturation. Association of plasma membrane activities with released vesicles (exosomes). J Biol Chem 262:9412–9420
Kowal J, Tkach M, Thery C (2014) Biogenesis and secretion of exosomes. Curr Opin Cell Biol 29:116–125
Reynolds ES (1963) The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J Cell Biol 17:208–212
Vermeer BJ et al (1978) Ultrastructural findings on lipoproteins in vitro and in xanthomatous tissue. Histochem J 10:299–307
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Chen, Y., Li, Y., Ma, L., Yu, L. (2018). Detection of Migrasomes. In: Gautreau, A. (eds) Cell Migration. Methods in Molecular Biology, vol 1749. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-7701-7_5
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DOI: https://doi.org/10.1007/978-1-4939-7701-7_5
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Publisher Name: Humana, New York, NY
Print ISBN: 978-1-4939-7700-0
Online ISBN: 978-1-4939-7701-7
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