Synopsis
The application of OsO4 plus K3[Fe(CN)6] as a secondary fixative following aldehyde fixation, permitted demonstration of the presence of 30–300 nm ‘membrane-bound’ particles in xanthomatous tissue.
With the same fixation method, isolated low density lipoprotein particles in a fibrin matrix could be observed in the transmission electron microscope in a way permitting comparison with similarly fixed tissue. However, isolated particles of very low density lipoproteins treated in the same way as low density particles had an irregular appearance and a diameter varying between 30 and 80 nm.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Braun Falco, O. (1973). Origin, structure, and function of the xanthoma cell.Nutr. Metabol. 15, 68–88.
Bruijn, W. C. De. (1969). De pathogenese van experimenteel verwekte atheromatose bij konijnen. Thesis, Leiden.
Bruijn, W. C. De, &Van Mourik, W. (1975). Scanning electron microscopic observations of endothelial changes in experimentally induced atheromatosis of rabbit aortas.Virch. Arch. A. 365, 23–40.
Bruijn, W. C. De &Den Breejen, P. (1975). Glycogen, its chemistry and morphological appearance in the electron microscope. II. The complex formed in the selective contrast staining of glycogen.Histochem. J.,7, 205–29.
Bijl, P. Van Der &Van Gent, C. M. (1975). Lipid and protein contents of low density lipoproteins (LDL) from patients with various types of hyperlipoproteinemia.Clin. Chim. Acta 63, 95–7.
Emeis, J. J., Van Gent, C. M. &Van Sabben, C. M. (1977). An enzymatic method for the histochemical localization of free and esterified cholesterol separately.Histochem. J. 9, 197–204.
Flickinger, C. J. (1967). The postnatal development of the sertoli cells of the mouse.Z. Zellforsch. Mikrosk. Anat. 78, 92–113.
Forte, T. &Nichols, A. V. (1972). Application of electron microscopy to the study of plasma lipoprotein structure.Adv. Lipid Res. 10, 1–41.
Frederickson, D. S. &Levy, R. I. (1972). Familial hyperlipoproteinemia. In:The metabolic basis of inherited diseases (eds. J. B. Stanbury, J. B. Wijngaarder & D. S. Fredrickson), 3rd edn, pp. 545–614. New York: McGraw-Hill.
Gent, C. M. Van (1972). Lipid composition of Lipoprotein Fractions. In:Protides of the biological fluids. (ed. H. Peeters) pp. 92–8. Oxford & New York: Pergamon Press.
Hessel, L. W., Vermeer, B. J., Polano, M. K., De Jonge, H., De Pagter, H. A. Th. &Van Gent, C. M. (1976). Primary hyperlipoproteinemia in xanthomatosis.Clinica Chim. Acta 69, 405–16.
Hoff, H. F. &Gaubatz, J. W. (1975). Ultrastructural localization of plasma lipoproteins in human intracranial arteries.Virchows Arch. A. 369, 111–21.
Jackson, R. L., Morrisett, J. D., &Gotto, A. M. (1976). Lipoprotein structure and metabolism.Phys. Rev. 56, 249–316.
Parker, F. &Odland, G. F. (1968). Experimental xanthoma: A correlative biochemical, histologic, histochemical and electron microscopic study.Am. J. Path. 53, 537–65.
Parker, F. &Odland, G. F. (1969). Electron microscopic similarities between experimental xanthomas and human eruptive xanthomas.J. invest. Derm. 52, 136–47.
Pasquali-Ronchetti, I., Calandra, S., Baccarani-Contri, M. &Montaguti, M. (1975). The ultrastructure of rat plasma lipoproteins.J. Ultrastruct. Res. 53, 180–92.
Polano, M. K., Baes, H., Hulsmans, H. A. M., Querido, A., Pries, C. &Van Gent, C. M. (1969). Xanthomata in primary hyperlipoproteinemia: A classification based on the lipoprotein pattern of the blood.Archs Derm. 100, 387–400.
Schneider, H. Morrod, R. S., Colvin, J. R. &Tattrie, N. H. (1973). The lipid core model of lipoproteins.Chem. Phys. Lipids. 10, 328–53.
Smith, E. B. &Slater, R. S. (1972). Relationship between low density lipoprotein in aortic intima and serum lipid levels.Lancet 1, 463–9.
Stein, Y. &Stein, O. (1973). Lipid synthesis and degradation and lipoprotein transport in mammalian aorta.Ciba Fdn Symp. 12, 165–84.
Vermeer, B. J., Van Gent, C. M., De Bruyn, W. C. &Boonders, T. (1978). The effect of digitonin-containing fixatives on the retention of free cholesterol and cholesterol esters.Histochem. J. 10, 287–298.
Walton, K. W., Thomas, C. &Dunkerley, D. J. (1973). The pathogenesis of xanthomata.J. Path. 109, 271–89.
Walton, K. W. Dunkerley, D. J. Johnson, A. G., Khan, M. K., Morris, C. &Watts, R. B. (1976). Investigations by immunofluorescence of arterial lesions in rabbits on two different lipid supplements and treated with pyridinol carbamate.Atherosclerosis 23, 117–39.
Wolff, H. H. &Braun Falco, O. (1970). Die Ultrastructur des Xanthelasma palpebrarum. Eine morphologische und electronen-mikroskopische, cytochemische. Untersuchung.Arch. klin. Exp. Derm. 238, 308–22.
World Health Organization, (1970). Classification of hyperlipidemias and hyperlipoproteinemias.Bull. Wld Hlth Org. 43, 891–907.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Vermeer, B.J., De Bruijn, W.C., Van Gent, C.M. et al. Ultrastructural findings on lipoproteinsin vitro and in xanthomatous tissue. Histochem J 10, 299–307 (1978). https://doi.org/10.1007/BF01007561
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF01007561