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Recombinant Protein Expression in E. coli : A Historical Perspective

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Heterologous Gene Expression in E.coli

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1586))

Abstract

This introductory chapter provides a brief historical survey of the key elements incorporated into commonly used E. coli-based expression systems. The highest impact in expression technology is associated with innovations that were based on extensively studied biological systems, and where the tools were widely distributed in the academic community.

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Authors and Affiliations

  1. Structural Genomics Consortium, University of Oxford, Old Road Campus Research Building, Roosevelt Drive, Headington, Oxford, OX3 7DQ, UK

    Opher Gileadi

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  1. Opher Gileadi
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Correspondence to Opher Gileadi .

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Editors and Affiliations

  1. SGC, Nuffield Department of Clinical Medicine, University of Oxford, Oxford, United Kingdom

    Nicola A. Burgess-Brown

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Gileadi, O. (2017). Recombinant Protein Expression in E. coli : A Historical Perspective. In: Burgess-Brown, N. (eds) Heterologous Gene Expression in E.coli. Methods in Molecular Biology, vol 1586. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6887-9_1

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  • DOI: https://doi.org/10.1007/978-1-4939-6887-9_1

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6885-5

  • Online ISBN: 978-1-4939-6887-9

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