Abstract
Plants are being developed as a cost-effective production system for biopharmaceuticals in large quantities. Although plants properly fold and assemble complex proteins from human origin, one issue that needs to be addressed is their glycan structure. In the past years we have been witnessing outstanding results in targeted manipulation of the plant N-glycosylation pathway allowing recombinant proteins to be produced with human-type oligosaccharides at large homogeneity. This opens new possibility in manufacturing next-generation biopharmaceuticals.
This review presents a variety of technologies and strategies that are being employed to engineer the plant N-glycosylation, thus pointing to the enormous potential of plants being used as a novel production system with unique features and possibilities.
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Abbreviations
- C1GalT1:
-
Drosophila melanogaster Core 1 synthase, glycoprotein-N-acetylgalactosamine 3-beta-galactosyltransferase 1
- CMAS:
-
Human CMP-N-acetylneuraminic acid synthase
- CST:
-
Mouse CMP-sialic acid transporter
- CTS:
-
Cytoplasmic tail, transmembrane domain, and stem
- FUT11:
-
A. thaliana α1,3-fucosyltransferase
- FUT8:
-
Human α1,6-fucosyltransferase
- FUT9a:
-
Human α1,3-fucosyltransferase IXa
- GalNAc-T2:
-
Human polypeptide N-acetylgalactosaminyltransferase
- GalT:
-
Human β1,4-galactosyltransferase
- GE:
-
Pseudomonas aeruginosa or Yersinia enterocolitica GMI, Golgi mannosidase I
- GMII:
-
Golgi mannosidase II
- GNE:
-
Mouse UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine-kinase
- GnT-I:
-
α1,3-Mannosyl-β1,2-N-acetylglucosaminyltransferase I
- GnT-II:
-
α1,6-Mannosyl-β1,2-N-acetylglucosaminyltransferase II
- GnT-III:
-
Human β1,4-mannosyl-β1,4-N-acetylglucosaminyltransferase III
- GnT-IV:
-
Human α1,3-mannosyl-β1,4-N-acetylglucosaminyltransferase IVa
- GnT-V:
-
Human α1,6-mannosyl-β1,6-N-acetylglucosaminyltransferase V
- HEXO1 and HEXO3:
-
Arabidopsis β-N-acetylhexosaminidases 1 and 3
- NANS:
-
Human N-acetylneuraminic acid phosphate-synthase
- P4H:
-
Prolyl-4-hydroxylase
- ST:
-
Rat or human α2,6-sialyltransferase
- ST3GalI:
-
Human α2,3-sialyltransferase
- ST6GalNAc4:
-
Mouse α2,6-sialyltransferase
- UDP-GlcNAc/UDP-GalNAc transporter:
-
UPD-GlcNAc 4-epimerase
- UGT:
-
Caenorhabditis elegans
- XylT:
-
β1,2-Xylosyltransferase
- α1,4-FucT:
-
α1,4-Fucosyltransferase
- β1,3-GalT:
-
β1,3-Galactosyltransferase
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Steinkellner, H., Castilho, A. (2015). N-Glyco-Engineering in Plants: Update on Strategies and Major Achievements. In: Castilho, A. (eds) Glyco-Engineering. Methods in Molecular Biology, vol 1321. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2760-9_14
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DOI: https://doi.org/10.1007/978-1-4939-2760-9_14
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