Overview
- Features protocols in glyco-engineering in bacteria, yeast, insects, plants and mammalian cells
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
Part of the book series: Methods in Molecular Biology (MIMB, volume 1321)
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About this book
Conceived with the intention of providing an array of strategies and technologies currently in use for glyco-engineering distinct living organisms, this book contains a wide range of methods being developed to control the composition of carbohydrates and the properties of proteins through manipulations on the production host rather than in the protein itself. The first five sections deal with host-specific glyco-engineering and contain chapters that provide protocols for modifications of the glycosylation pathway in bacteria, yeast, insect, plants and mammalian cells, while the last two sections explore alternative approaches to host glyco-engineering and selected protocols for the analysis of the N-glycans and glyco-profiling by mass spectrometry. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.
Authoritative and extensive, Glyco-Engineering: Methods and Protocols offers vast options to help researchers to choose the expression system and approach that best suits their intended protein research or applications.
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Keywords
Table of contents (29 protocols)
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Glyco-Engineering in Bacteria
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Glyco-Engineering in Yeast
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Glyco-Engineering in Insects
Editors and Affiliations
Bibliographic Information
Book Title: Glyco-Engineering
Book Subtitle: Methods and Protocols
Editors: Alexandra Castilho
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-4939-2760-9
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media New York 2015
Hardcover ISBN: 978-1-4939-2759-3Published: 17 June 2015
Softcover ISBN: 978-1-4939-4942-7Published: 09 October 2016
eBook ISBN: 978-1-4939-2760-9Published: 16 June 2015
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XVI, 439
Number of Illustrations: 27 b/w illustrations, 52 illustrations in colour
Topics: Protein Science