Abstract
Swimming motility is a flagellum-dependent form of movement observed in the Gram-negative bacterium Pseudomonas aeruginosa. Swimming motility is defined as the movement in liquid or low-viscosity conditions (up to 0.3 % agar concentration). Unlike swarming motility, swimming motility requires a functional flagellum, but neither quorum sensing (QS) systems nor biosurfactants. While swimming motility can also be observed via microscopy, here we describe a reproducible plate-based method.
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Acknowledgement
This work was supported by the NIH grant R01A1003256 to G.A.O. and the Rosaline Borison predoctoral fellowship awarded to D.G.H.
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Ha, DG., Kuchma, S.L., O’Toole, G.A. (2014). Plate-Based Assay for Swimming Motility in Pseudomonas aeruginosa . In: Filloux, A., Ramos, JL. (eds) Pseudomonas Methods and Protocols. Methods in Molecular Biology, vol 1149. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-0473-0_7
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DOI: https://doi.org/10.1007/978-1-4939-0473-0_7
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Publisher Name: Humana, New York, NY
Print ISBN: 978-1-4939-0472-3
Online ISBN: 978-1-4939-0473-0
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