Abstract
Proteus mirabilis is well known for using its flagella to swim through liquids or swarm across solid surfaces. Both phenomena are easy to observe. Described here are two agar-based assays for studying both swimming and swarming behavior, and considerations that affect the outcome.
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References
Schaffer JN, Pearson MM (2015) Proteus mirabilis and urinary tract infections. Microbiol Spectr 3(5). https://doi.org/10.1128/microbiolspec.UTI-0017-2013
Macnab RM (1996) Flagella and motility. In: Neidhardt FC (ed) Escherichia coli and Salmonella: Cellular and molecular biology, vol 1, 2nd edn. ASM Press, Washington, DC, pp 123–145
Harshey RM (2003) Bacterial motility on a surface: many ways to a common goal. Annu Rev Microbiol 57:249–273. https://doi.org/10.1146/annurev.micro.57.030502.091014
Kearns DB (2010) A field guide to bacterial swarming motility. Nat Rev Microbiol 8(9):634–644. https://doi.org/10.1038/nrmicro2405
Jones BV, Young R, Mahenthiralingam E, Stickler DJ (2004) Ultrastructure of Proteus mirabilis swarmer cell rafts and role of swarming in catheter-associated urinary tract infection. Infect Immun 72(7):3941–3950
Armbruster CE, Prenovost K, Mobley HL, Mody L (2017) How often do clinically diagnosed catheter-associated urinary tract infections in nursing homes meet standardized criteria? J Am Geriatr Soc 65(2):395–401. https://doi.org/10.1111/jgs.14533
Warren JW, Tenney JH, Hoopes JM, Muncie HL, Anthony WC (1982) A prospective microbiologic study of bacteriuria in patients with chronic indwelling urethral catheters. J Infect Dis 146(6):719–723
Tittsler RP, Sandholzer LA (1936) The use of semi-solid agar for the detection of bacterial motility. J Bacteriol 31(6):575–580
Armbruster CE, Hodges SA, Mobley HLT (2013) Initiation of swarming motility by Proteus mirabilis occurs in response to specific cues present in urine and requires excess L-glutamine. J Bacteriol 195(6):1305–1319. https://doi.org/10.1128/JB.02136-12
Li X, Rasko DA, Lockatell CV, Johnson DE, Mobley HLT (2001) Repression of bacterial motility by a novel fimbrial gene product. EMBO J 20(17):4854–4862
Pearson MM, Mobley HLT (2008) Repression of motility during fimbrial expression: identification of 14 mrpJ gene paralogues in Proteus mirabilis. Mol Microbiol 69(2):548–558
Coetzee JN, Sacks TG (1960) Morphological variants of Proteus hauseri. J Gen Microbiol 23:209–216. https://doi.org/10.1099/00221287-23-2-209
Pearson MM, Rasko DA, Smith SN, Mobley HLT (2010) Transcriptome of swarming Proteus mirabilis. Infect Immun 78(6):2834–2845. https://doi.org/10.1128/IAI.01222-09
Allison C, Lai HC, Hughes C (1992) Co-ordinate expression of virulence genes during swarm-cell differentiation and population migration of Proteus mirabilis. Mol Microbiol 6(12):1583–1591
Walker KE, Moghaddame-Jafari S, Lockatell CV, Johnson D, Belas R (1999) ZapA, the IgA-degrading metalloprotease of Proteus mirabilis, is a virulence factor expressed specifically in swarmer cells. Mol Microbiol 32(4):825–836
Alteri CJ, Himpsl SD, Engstrom MD, Mobley HLT (2012) Anaerobic respiration using a complete oxidative TCA cycle drives multicellular swarming in Proteus mirabilis. mBio 3(6). doi:https://doi.org/10.1128/mBio.00365-12
Allison C, Lai HC, Gygi D, Hughes C (1993) Cell differentiation of Proteus mirabilis is initiated by glutamine, a specific chemoattractant for swarming cells. Mol Microbiol 8(1):53–60
Rauprich O, Matsushita M, Weijer CJ, Siegert F, Esipov SE, Shapiro JA (1996) Periodic phenomena in Proteus mirabilis swarm colony development. J Bacteriol 178(22):6525–6538
Kvittingen J (1949) Studies of the life-cycle of Proteus Hauser. Acta Pathol Mic Sc 26(1):24–50
Pearson MM, Sebaihia M, Churcher C, Quail MA, Seshasayee AS, Luscombe NM, Abdellah Z, Arrosmith C, Atkin B, Chillingworth T, Hauser H, Jagels K, Moule S, Mungall K, Norbertczak H, Rabbinowitsch E, Walker D, Whithead S, Thomson NR, Rather PN, Parkhill J, Mobley HLT (2008) Complete genome sequence of uropathogenic Proteus mirabilis, a master of both adherence and motility. J Bacteriol 190(11):4027–4037
Daum B, Gold V (2018) Twitch or swim: towards the understanding of prokaryotic motion based on the type IV pilus blueprint. Biol Chem 399(7):799–808. https://doi.org/10.1515/hsz-2018-0157
Hazelbauer GL, Mesibov RE, Adler J (1969) Escherichia coli mutants defective in chemotaxis toward specific chemicals. Proc Natl Acad Sci U S A 64(4):1300–1307
Belas R, Schneider R, Melch M (1998) Characterization of Proteus mirabilis precocious swarming mutants: identification of rsbA, encoding a regulator of swarming behavior. J Bacteriol 180(23):6126–6139
Gygi D, Rahman MM, Lai HC, Carlson R, Guard-Petter J, Hughes C (1995) A cell-surface polysaccharide that facilitates rapid population migration by differentiated swarm cells of Proteus mirabilis. Mol Microbiol 17(6):1167–1175
Matsuyama T, Takagi Y, Nakagawa Y, Itoh H, Wakita J, Matsushita M (2000) Dynamic aspects of the structured cell population in a swarming colony of Proteus mirabilis. J Bacteriol 182(2):385–393
Acknowledgments
Thank you to Stephanie Himpsl and Chelsie Armbruster for adding suggestions to these methods.
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Pearson, M.M. (2019). Methods for Studying Swarming and Swimming Motility. In: Pearson, M. (eds) Proteus mirabilis. Methods in Molecular Biology, vol 2021. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9601-8_3
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DOI: https://doi.org/10.1007/978-1-4939-9601-8_3
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