Abstract
Because specific treatment for trichomoniasis is available, it is important to identify infected people so that disease in the individual can be terminated and the sexual transmission of the organism prevented. The classic symptoms of the disease in women are a thin yellow offensive vaginal discharge, pruritus vulvae or vulval soreness, dyspareunia, and dysuria. A reddened vaginal wall with frothy thin yellow exudate in the posterior fornix and punctate red spots on the ectocervix (“strawberry cervix”) may be seen.1 These features, however, are not always present and are not pathognomonic of trichomoniasis. Although about 50% of women infected with T. vaginalis notice an increased vaginal discharge, a similar proportion of non-infected women give a similar history.23 Since about 18% and 12% of infected and nonin-fected women respectively have dysuria, this is not a helpful diagnostic feature.2 An abnormal discharge in the vaginal fornices may be seen more frequently in infected women (about 50%) than in noninfected individuals (about 22%),3 but this is described as “frothy” in only 12% of cases.2 The strawberry cervix is rarely seen.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
Similar content being viewed by others
References
King A, Nicol C: Venereal Diseases. London: Baillière, Tindall, 1969.
Fouts AC, Kraus SJ: Trichomonas vaginalis: Re-evaluation of its clinical presentation and laboratory diagnosis. J Infect Dis 141:133–143, 1980.
McLellan R, Spence MR, Broekman M, Raffel PA-C, Smith JL: The clinical diagnosis of trichomoniasis. Obstet Gynecol 60:30–34, 1982.
Robertson DHH, Lumsden WHR, Fräser KF, Hosie DD, Moore DM: Simultaneous isolation of Trichomonas vaginalis and collection of vaginal exudate. Br J Vener Dis 45:42–43, 1969.
Oates JK, Selwyn S, Breach MR: Polyester sponge swabs to facilitate examination for genital infection in women. Br J Vener Dis 47:289–292, 1971.
Hoffman B, Mafyszko E: Studies on the way of laboratory diagnosis of trichomonadosis in men. Wiad Parazytol 8:179–189, 1962.
Stuart RD: Transport problems in public health bacteriology. Can J Public Health 47:114–122, 1956.
Amies CR: A modified formula for the preparation of Stuart’s transport medium. Can J Public Health 58:296–300, 1967.
Nielsen R: Trichomonas vaginalis I. Survival in solid Stuart’s medium. Br J Vener Dis 45:328–331, 1969.
O’Connor BH, Adler MW: Current approaches to the diagnosis, treatment and reporting of trichomoniasis and candidosis. Br J Vener Dis 55:52–57, 1979.
Kean BH, Day E: Trichomonas vaginalis infection: An evaluation of three diagnostic techniques with data on incidence. Am J Obstet Gynecol 68:1510–1518, 1954.
Feinberg JG, Whittington MJ: A culture medium for Trichomonas vaginalis Donné and species of Candida. J Clin Pathol 10:327–329, 1957.
Clark DH, Solomons E: An evaluation of routine culture examination for Trichomonas vaginalis and Candida. Am J Obstet Gynecol 78:1314–1319, 1959.
Martin RD, Kaufman RH, Burns M: Trichomonas vaginalis vaginitis: A statistical evalua-tion of diagnostic methods. Am J Obstet Gynecol 87:1024–1027, 1963.
Hulka BS, Hulka JF: Dyskaryosis in cervical cytology and its relationship to trichomoniasis therapy. Am J Obstet Gynecol 98:180–187, 1967.
Rayner CFA: Comparison of culture media for the growth of Trichomonas vaginalis. Br J Vener Dis 44:63–66, 1968.
Nagesha CN, Ananthakrishna NC, Sulochana P: Clinical and laboratory studies on vaginal trichomoniasis. Am J Obstet Gynecol 106:933–935, 1970.
McCann JS: Comparison of direct microscopy and culture in the diagnosis of trichomoniasis. Br J Vener Dis 50:450–452, 1974.
Eriksson G, Wanger L: Frequency of N. gonorrhoeae, T. vaginalis and C. albicans in female venereological patients. Br J Vener Dis 51:192–197, 1975.
Rothenberg RB, Simon R, Chipperfield E, Catterall RD: Efficacy of selected diagnostic tests for sexually transmitted diseases. JAMA 235:49–51, 1976.
Spence MR, Hollander DH, Smith J, McCaig L, Sewell D, Broekman M: The clinical and laboratory diagnosis of Trichomonas vaginalis infection. Sex Transm Dis 7:168–171, 1980.
Hoffman B, Kilczewski W, Malyszko E: Studies in trichomoniasis in males. Br J Vener Dis 37:172–175, 1961.
Miller JR: Contrast stain for the rapid identifi cation of Trichomonas vaginalis. JAMA 106: 616, 1936.
Eddie DAS: The laboratory diagnosis of vaginal infections caused by Trichomonas and Candida (Monilia) species. J Med Microbiol 1:153–159, 1968.
Perju A, Strïmbeanu I: Valeur de la spermocul-ture dans le diagnostic de la trichomonase urogenitale chez rhomme. Wiad Parazytol 12:475–480, 1966.
Coutts WE, Silva-Inzunza E: Vital staining of Trichomonas vaginalis with fluorescein. Br J Vener Dis 30:43–46, 1954.
Barchet S : A new look at vaginal discharges. Obstet Gynecol 40:615–617, 1972.
Sobrepena RL: Identification of Trichomonas vaginalis in Gram-stained smears. Lab Med 11:558–560, 1980.
Cree GE: Trichomonas vaginalis in Gram-stained smears. Br J Vener Dis 44:226–227, 1968.
Oller LZ: Routine exfoliative cytology for cancer and Trichomonas detection at a clinic for venereal diseases. Br J Vener Dis 41:304–308, 1965.
Hughes HE, Gordon AM, Barr GTD: A clinical and laboratory study of trichomoniasis of the female genital tract. J Obstet Gynaecol Br Commons 73:821–827, 1966.
Thin RNT, Atia W, Parker JDJ, Nicol CS, Canti G: Value of Papnicolaou-stained smears in the diagnosis of trichomoniasis, candidiasis and cervical herpes simplex virus infection in women. Br J Vener Dis 51:116–118, 1975.
Mason PR, Super H, Fripp PJ: Comparison of four techniques for the routine diagnosis of Trichomonas vaginalis infection. J Clin Pathol 29:154–157, 1976.
Perl G: Errors in the diagnosis of Trichomonas vaginalis infection as observed among 1199 patients. Obstet Gynecol 39:7–9, 1972.
Summers JL, Ford ML: The Papanicolaou smear as a diagnostic tool in male trichomoni-asis. J Urol 107:840–842, 1972.
Kurpiel M : Pictures of T. vaginalis obtained with modified method of staining cytological preparations. Wiad Parazytol 23:491–495, 1977.
Sorel C: Trois techniques de recherche du Trichomonas vaginalis’ leurs valeurs compar-ees. Presse Méd 62:602–604, 1954.
Lowe GH: A comparison of current laboratory methods with a new semisolid culture medium for the detection of Trichomonas vaginalis. J Clin Pathol 18:432–434, 1965.
Rogers S, Goldsmid JM: A study of the possible value of acridine orange-O stain in the diagnosis of Trichomonas vaginalis infection. Cent Afr J Med 23:56–58, 1977.
Buharowski K, Wolahska M: Usefulness of samples stained with acridine orange for diagnosis of women’s trichomonadosis. Wiad Parazytol 30:499–501, 1984.
Levett PN: A comparison of five methods for the detection of Trichomonas vaginalis in clinical specimens. Med Lab Sci 37:85–88, 1980.
Fripp PJ, Mason PR, Super H: A method for the diagnosis of Trichomonas vaginalis using acridine orange. J Parasitol 61:966–967, 1975.
van Niekerk WA: Acridine-orange fluorescence microscopy in the detection of pathogenic vaginal flora. Obstet Gynecol 20:596–601, 1962.
Maciejewski Z, Dziecielski H: Method of secondary fluorescence in diagnostics of tricho-monadosis and in control of the effectiveness of antitrichomonal therapy. Wiad Parazytol 23:617–619, 1977.
Hipp SS, Kirkwood MW, Gaafar HA: Screening for Trichomonas vaginalis infection by use of acridine orange fluorescent microscopy. Sex Transm Dis 6:235–238, 1979.
Greenwood JR, Kirk-Hillaire K: Evaluation of acridine orange stain for detection of Trichomonas vaginalis in vaginal specimens. J Clin Microbiol 14:699, 1981.
Ridge AG: A rapid method for detection of Trichomonas vaginalis. Med Lab Sci 39:193–194, 1982.
Honigberg BM, Davenport HA: Staining flagellate protozoa by various silver protein compounds. Stain Technol 29:241–246, 1954.
Franceschini Ph: Nouvelle méthode de coloration du Trichomonas. Presse Méd 79:486–487, 1971.
Klugiewicz A : A comparison of two methods of staining of Trichomonas vaginalis for the needs of medical diagnosis and didactics. Wiad Parazyto l20:517–520, 1974.
Balsdon MJ, Green N, Andrew CW, Jackson DH: Rapid staining technique for Trichomonas vaginalis. Br J Vener Dis 55:289–291, 1979.
Rodriguez-Martinez HA, Rosales de la Luz M, de Bello LG, Ruiz-Moreno JA: Adequate staining of Trichomonas vaginalis by McManus periodic acid-Schiff stain. Am J Clin Pathol 59:741–746, 1973.
McEntegart MG, Chadwick CS, Nairn RC: Fluorescent antisera in the detection of serological varieties of Trichomonas vaginalis. Br J Vener Dis 34:1–3, 1958.
Hayes BS, Koteher E: Evaluation of techniques for the demonstration of Trichomonas vaginalis. J Parasitol 46(suppl):45, 1960.
Bennett BD, Bailey J, Gardner WA: Immunocytochemical identification of trichomonads. Arch Pathol Lab Med 104:247–249, 1980.
O’Hara CM, Gardner WA, Bennett BD: Immu-noperoxidase staining of Trichomonas vaginalis in cytologic material. Acta Cytol 24:448–451, 1980.
Taylor AER, Baker JR: The Cultivation of Parasites in Vitro. Oxford: Black well, 1968.
Lumsden WHR, Robertson DHH, McNeillage GJC: Isolation, cultivation, low temperature preservation, and infectivity titration of Trichomonas vaginalis. Br J Vener Dis 42:145–154, 1966.
Johnson G, Trussel RE: Experimental basis for the chemotherapy of Trichomonas vaginalis infestations. Proc Soc Exp Biol Med 54:245–249, 1943.
Diamond LS: The establishment of various trichomonads of animals and man in axenic cultures. J Parasitol 43:488–490, 1957.
Cox PJ, Nicol CS: Growth studies of various strains of T. vaginalis and possible improve-ments in the laboratory diagnosis of trichomoniasis. Br J Vener Dis 49:536–539, 1973.
Trussel RE : Trichomonas vaginalis and Trichomoniasis. Springfield, I11: Charles C Thomas, 1947.
Hoffman B, Fiedoruk J: Studies of the carbohydrate metabolism of T. vaginalis. Wiad Parazytol 12:299–303, 1966.
Jaakmees HP, Teras JK, Röigas EM, Nigesen UK, Tompel HJ: Complement fixing antibodies in the blood sera of men infested with Trichomonas vaginalis. Wiad Parazytol 12:378–384, 1966.
Hoffmann B, Kazanowska W, Kilczewski W, Krach J: Serologic diagnosis of Trichomonas infection. Med Dósw Mikrobiol 15:91–99, 1963.
Teras JK, Jaakmees HP, Nigesen UK, Röigas EM, Tompel HJ: The dependence of serologic reactions on the serotypes of Trichomonas vaginalis. Wiad Parazytol 12:364–369, 1966.
McEntegart MG: The application of a haem-agglutination technique in the study of Trichomonas vaginalis infections. J Clin Pathol 5:275–280, 1952.
Hoffmann B: An evaluation of the use of the indirect hemagglutination method in the serodi-agnosis of trichomonadosis. Wiad Parazytol 12:392–397, 1966.
Kuberski T: Evaluation of the indirect hemagglutination technique for study of Trichomonas vaginalis infections, particularly in men. Sex Transm Dis 5:97–102, 1978.
Kramäf J, Kucera K: Immunofluorescence demonstration of antibodies in urogenital trichomoniasis. J Hyg Epidemiol Microbiol Immunol 10:85–88, 1966.
Street DA, Taylor-Robinson D, Ackers JC, Hanna NF, McMillan A: Evaluation of an enzyme-linked immunosorbent assay for the detection of antibody to Trichomonas vaginalis in sera and vaginal secretions. Br J Vener Dis 58:330–333, 1982.
Treharne JD, Darougar S, Simmons PD, Thin RN: Rapid diagnosis of chlamydial infection of the cervix. Br J Vener Dis 54:403–408, 1978.
Ackers JC, Catterall RD, Lumsden WHR, McMillan A: Absence of detectable local antibody in genitourinary tract secretions of male contacts of women infected with Trichomonas vaginalis. Br J Vener Dis 54:168–171, 1977.
Adler S, Sadowsky A: Intradermal reaction in trichomonas infection. Lancet 1:867–868, 1947.
Kawai N, Ishibashu J, Watanabe A, Etsura H: Intradermal test for trichomoniasis in women. Sanfujinka No Sekai (World of Obstetrics and Gynecology) 13:1177–1181, 1961.
Jaakmees HP, Teras JK: Intradermal reaction with specific antigens in cases of genitourinary trichomonadosis. Wiad Parazytol 12:385–391, 1966.
Lumsden WHR, McMillan A: Protozoa. In Col-lee JG, Duguid JC (eds): Mackie and McCartney: Medical Microbiology, 13th ed, vol 2. Edinburgh: Churchill Livingstone, 1987.
Robinson GL: The laboratory diagnosis of human parasitic amoebae. Trans R Soc Trop Med Hyg 62:285–294, 1968.
Markeil EK, Voge M: Medical Parasitology, Philadelphia: WB Saunders, 1976.
Honigberg BM : Trichomonads of importance in human medicine. In Kreier JP (ed): Parasitic Protozoa, vol 2, pp 275–454. New York: Academic Press, 1978.
Lindgren RD, Ivey MH: The effect of cultivation and freezing on the virulence of Trichomonas vaginalis for mice. J Parasitol 50:226–229, 1964.
Ivey MH: Virulence preservation of recent isolates of Trichomonas vaginalis. J Parasitol 61:550–552, 1975.
Lumsden WHR, Hardy GJC: Nomenclature of living parasite material. Nature 205:1032, 1965.
Diamond LS, Bartgis, IL, Reardon LV: Virulence of Trichomonas vaginalis after freeze-pres-ervation for 2 years in liquid nitrogen vapor. Cryobiology 1:295–297, 1965.
Wasley GD, Rayner CFA: Preservation of Trichomonas vaginalis in liquid nitrogen. Br J Vener Dis 46:323–325, 1970.
Ivey MH: Use of solid medium techniques to evaluate factors affecting the ability of Trichomonas vaginalis to survive freezing. J Parasitol 61:1101–1103, 1975.
McEntegart MG: The maintenance of stock strains of trichomonads by freezing. J Hyg 52:545–550, 1954.
Diamond LS: Freeze-preservation of protozoa. Cryobiology 1:95–102, 1964.
Honigberg BM, Farris VK, Livingston MC: Preservation of Trichomonas vaginalis and Trichomonas gallinae in liquid nitrogen. Prog Protozool, Proc II Int Conf Protozool, 1965. Excerpta Med Found, Int Congr Ser 91:199, 1965.
Lumsden WHR, Herbert WJ, McNeillage GJC: Techniques with Trypanosomes. Edinburgh: Churchill Livingstone, 1973.
Müller W: Zur Frage der Konservierung von Laborstämmen verschiedener Trichomonas und Trichomonasarten durch tiefe Temperaturen. Z Tropenmed Parasitol 17:100–102, 1966.
Kasprzak W, Rydzewski A: Preservation of some parasitic protozoa at low temperatures. Z Tropenmed Parasitol 21:198–201, 1970.
LeCorroller Y, Gysin J, l’Herete P: Une technique simple de conservation des protozoaires par congelation. Ann Inst Pasteur (Algérie) 48:109–124, 1970.
Diamond LS, Bartgis IL: Axenic cultures for in vitro testing of drugs against Entamoeba histolytica. Arch Invest Med 2(suppl):339–348, 1971.
Diamond LS: Axenic cultivation of Trichomonas tenax, the oral flagellate of man. I. Es-tablishment of cultures. J Protozool 9:442–444, 1962.
Krieger JN, Holmes KK, Spence MR, Rein MF, McCormack WM, Tarn MR: Geographic variation among isolates of Trichomonas vaginalis: Demonstration of antigenic heterogeneity by using monoclonal antibodies and the indirect immunofluorescence technique. J Infect Dis 152: 979–984, 1985.
Chang TH, Tsing SY, Tzeng S: Monoclonal antibodies against Trichomonas vaginalis. Hybridoma 5:43–51, 1986.
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1990 Springer-Verlag New York Inc.
About this chapter
Cite this chapter
McMillan, A. (1990). Laboratory Diagnostic Methods and Cryopreservation of Trichomonads. In: Honigberg, B.M. (eds) Trichomonads Parasitic in Humans. Springer, New York, NY. https://doi.org/10.1007/978-1-4612-3224-7_16
Download citation
DOI: https://doi.org/10.1007/978-1-4612-3224-7_16
Publisher Name: Springer, New York, NY
Print ISBN: 978-1-4612-7922-8
Online ISBN: 978-1-4612-3224-7
eBook Packages: Springer Book Archive