The dimorphic ascomycetous yeast Arxula adeninivorans exhibits some unusual properties. Being a thermo- and halotolerant species it is able to assimilate and ferment many compounds as sole carbon and/or nitrogen source. It utilises n-alkanes and is capable of degrading starch. Due to these unusual biochemical properties A. adeninivorans can be exploited as a gene donor for the production of enzymes with attractive biotechnological characteristics. Examples of A. adeninivorans-derived genes that are overexpressed include the ALIP1 gene encoding a secretory lipase, the AINV encoding invertase, the AXDH encoding xylitol dehydrogenase and the APHY encoding a secretory phosphatase with phytase activity.
The thermo- and halotolerance as well as differential morphology-dependent glycosylation and the secretion characteristics render A. adeninivorans attractive as host for heterologous gene expression. A transformation system has been established based on homologous integration of linearised DNA fragments. Successful expression examples like that of the E. coli-derived lacZ gene, GFP and human HSA and IL6 genes add to the attraction of A. adeninivorans as host for hetero logous gene expression.
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Böer, E., Steinborn, G., Florschütz, K., Körner, M., Gellissen, G., Kunze, G. (2009). Arxula adeninivorans (Blastobotrys adeninivorans) — A Dimorphic Yeast of Great Biotechnological Potential. In: Satyanarayana, T., Kunze, G. (eds) Yeast Biotechnology: Diversity and Applications. Springer, Dordrecht. https://doi.org/10.1007/978-1-4020-8292-4_27
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