Abstract
In this chapter we summarize the results of reports published since 1979 on the Chl a/b-binding light-harvesting complex of PS I. In the first part of this chapter, we review the results that led to our current knowledge of the biochemical properties of the individual gene products constituting LHCI, in particular, the presence and distribution of red-shifted spectral forms and the idea that LHCI, different from LHCII, is organized into heterodimeric complexes.
Access provided by Autonomous University of Puebla. Download to read the full chapter text
Chapter PDF
Similar content being viewed by others
Keywords
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
References
Allen JF (1992) How does protein phosphorylation regulate photosynthesis? Trends Biochem Sci 17: 12–17
Bassi R (1985) Spectral properties and polypeptide composition of the chlorophyll–proteins from thylakoids of granal and agranal chloroplasts of maize (zea mays L.). Carlsberg Res Commun 50: 127–143
Bassi R and Simpson D (1987) Chlorophyll–protein complexes of barley photosystem I. Eur J Biochem 163: 221–230
Bassi R, Machold O and Simpson D (1985) Chlorophyll–proteins of two photosystem I preparations from maize. Carlsberg Res Commun 50: 145–162
Bassi R, Soen SY, Frank G, Zuber H and Rochaix JD (1992) Characterization of chlorophyll-a/b proteins of photosystem-I from Chlamydomonas reinhardtii. J Biol Chem 267: 25714–25721
Bassi R, Pineau B, Dainese P and Marquardt J (1993) Carotenoid-binding proteins of Photosystem-II. Eur J Biochem 212: 297–303
Bassi R, Croce R, Cugini D and Sandona D (1999) Mutational analysis of a higher plant antenna protein provides identification of chromophores bound into multiple sites. Proc Natl Acad Sci USA 96: 10056–10061
Ben Shem A, Frolow F and Nelson N (2003) Crystal structure of plant photosystem I. Nature 426: 630–635
Bibby TS, Nield J and Barber J (2001) Iron deficiency induces the formation of an antenna ring around trimeric photosystem I in cyanobacteria. Nature 412: 743–745
Boardman NK, Anderson JM and Goodchild DJ (1978) Chlorophyll–protein complexes and structure of mature and developing chloroplast. In: Sanadi DR and Vernon LP (eds) Current Topics in Bioenergeties, Vol 8, pp 35–109. Academic Press, New York
Boekema EJ, Wynn RM and Malkin R (1990) The structure of spinach photosystem I studied by electron microscopy. Biochim Biophys Acta 1017: 49–56
Boekema EJ, van Roon H, Calkoen F, Bassi R and Dekker JP (1999) Multiple types of association of photosystem II and its light-harvesting antenna in partially solubilized photosystem II membranes. Biochemistry 38: 2233–2239
Boekema EJ, Hifney A, Yakushevska AE, Piotrowski M, Keegstra W, Berry S, Michel KP, Pistorius EK and Kruip J (2001a) A giant chlorophyll–protein complex induced by iron deficiency in cyanobacteria. Nature 412: 745–748
Boekema EJ, Jensen PE, Schlodder E, van Breemen JF, van Roon H, Scheller HV and Dekker JP (2001b) Green plant photosystem I binds light-harvesting complex I on one side of the complex. Biochemistry 40: 1029–1036
Bossmann B, Knoetzel J and Jansson S (1997) Screening of chlorina mutants of barley (Hordeum vulgare L.) with antibodies against light-harvesting proteins of PS I and PS II: absence of specific antenna proteins. Photosynth Res 52: 127–136
Butler WL and Norris KH (1963) Lifetime of the long-wavelength chlorophyll fluorescence. Biochim Biophys Acta 66: 72–77
Butler WL, Tredwell CJ, Malkin R and Barber J (1979) The relationship between the lifetime and yield of the 735 nm fluorescence of chloroplasts at low temperatures. Biochim Biophys Acta 545: 309–315
Castelletti S, Morosinotto T, Robert B, Caffarri S, Bassi R and Croce R (2003) Recombinant Lhca2 and Lhca3 subunits of the photosystem I antenna system. Biochemistry 42: 4226–4234
Croce R (1997) Il fotosistema I delle piante superiori: studi biochimici e spettroscopici. Ph.D. thesis, Universitá di Milano, Milano
Croce R and Bassi R (1998) The light-harvesting complex of photosystem I: pigment composition and stoichiometry. In: Garab G (ed) Photosynthesis: Mechanisms and Effects, Vol 1, pp 421–424. Kluwer Academic Press, Dordrecht
Croce R, Zucchelli G, Garlaschi FM, Bassi R and Jennings RC (1996) Excited state equilibration in the photosystem I light-harvesting I complex: P700 is almost isoenergetic with its antenna. Biochemistry 35: 8572–8579
Croce R, Zucchelli G, Garlaschi FM and Jennings RC (1998) A thermal broadening study of the antenna chlorophylls in PSI-200, LHCI, and PSI core. Biochemistry 37: 17255–17360
Croce R, Remelli R, Varotto C, Breton J and Bassi R (1999) The neoxanthin binding site of the major light harvesting complex (LHC II) from higher plants. FEBS Lett 456: 1–6
Croce R, Morosinotto T, Castelletti S, Breton J and Bassi R (2002) The Lhca antenna complexes of higher plants photosystem I. Biochim Biophys Acta 1556: 29–40
Croce R, Muller MG, Bassi R and Holzwarth AR (2003) Chlorophyll b to chlorophyll a energy transfer kinetics in the CP29 antenna complex: a comparative femtosecond absorption study between native and reconstituted proteins. Biophys J 84: 2508–2516
Damm I, Steinmetz D and Grimme LH (1990) Multiple functions of β β-carotene in Photosystem I. In: Baltscheffsky M (ed) Current Research in Photosynthesis, pp 607–610. Kluwer Academic Press, Dordrecht
Dunahay TG and Staehelin A (1985) Isolation of photosystem I complexes from octyl glucoside/sodium dodecyl sulfate solubilized spinach thylakoids. Characterization and reconstitution into liposomes. Plant Physiol 78: 606–613
Durnford DG, Deane JA, Tan S, McFadden GI, Gantt E and Green BR (1999) A phylogenetic assessment of the eukaryotic light-harvesting antenna proteins, with implications for plastid evolution. J Mol Evol 48: 59–68
Ganeteg U, Strand A, Gustafsson P and Jansson S (2001) The properties of the chlorophyll a/b-binding proteins Lhca2 and Lhca3 Studied in vivo using antisense inhibition. Plant Physiol 127: 150–158
Germano M, Yakushevska AE, Keegstra W, van Gorkom HJ, Dekker JP and Boekema EJ (2002) Supramolecular organization of photosystem I and light-harvesting complex I in Chlamydomonas reinhardtii. FEBS Lett 525: 121–125
Giuffra E, Cugini D, Croce R and Bassi R (1996) Reconstitution and pigment-binding properties of recombinant CP29. Eur J Biochem 238: 112–120
Gobets B and van Grondelle R (2001) Energy transfer and trapping in photosystem I. Biochim Biophys Acta 1057: 80–99
Gobets B, Van Amerongen H, Monshouwer R, Kruip J, Rögner M, van Grondelle R and Dekker JP (1994) Polarized site-selected fluorescence spectroscopy of isolated photosystem I particles. Biochim Biophys Acta 1188: 75–85
Green BR and Durnford DG (1996) The chlorophyll–carotenoid proteins of oxygenic photosynthesis. Annu Rev Plant Physiol Plant Mol Biol 47: 685–714
Havaux M, Dall’Osto L, Cuiné S, Giuliano G and Bassi R (2004) The effect of zeaxanthin as the only xanthophyll on the structure and function of the photosynthetic apparatus in Arabidopsis thaliana.J Biol Chem 279: 13878–13888
Haworth P, Watson JL and Arntzen CJ (1983) The detection, isolation and characterisation of a light-harvesting complex which is specifically associated with photosystem I. Biochim Biophys Acta 724: 151–158
Ihalainen JA, Gobets B, Sznee K, Brazzoli M, Croce R, Bassi R, van Grondelle R, Korppi-Tommola JEI and Dekker JP (2000) Evidence for two spectroscopically different dimers of light-harvesting complex I from green plants. Biochemistry 39: 8625–8631
Ihalainen JA, Rätsep M, Jensen PE, Scheller HV, Croce R, Bassi R, Korppi-Tommola JEI and Freiberg A (2003) Red spectral forms of chlorophylls in green plant PSI –a site-selective and high-pressure spectroscopy study. J Phys Chem B 107: 9086–9093
Ikeuchi M, Hirano A and Inoue Y (1991a) Correspondence of apoproteins of light-harvesting chlorophyll-a/b complexes associated with photosystem-I to cab genes –evidence for a novel type-IV apoprotein. Plant Cell Physiol 32: 103–112
Ikeuchi M, Nyhus KJ, Inoue Y and Pakrasi HB (1991b) Identities of four low-molecular-mass subunits of the photosystem I complex from Anabaena variabilis ATCC 29413. Evidence for the presence of the psaI gene product in a cyanobacterial complex. FEBS Lett 287: 5–9
Jansson S (1994) The light-harvesting chlorophyll a/b-binding proteins. Biochim Biophys Acta 1184: 1–19
Jansson S (1999) A guide to the Lhc genes and their relatives in Arabidopsis. Trends Plant Sci 4: 236–240
Jansson S, Andersen B and Scheller HV (1996) Nearest-neighbor analysis of higher-plant photosystem I holocomplex. Plant Physiol 112: 409–420
Jensen PE, Gilpin M, Knoetzel J and Scheller HV (2000) The PSI-K subunit of photosystem I is involved in the interaction between light-harvesting complex I and the photosystem I reaction center core. J Biol Chem 275: 24701–24708
Jordan P, Fromme P, Witt HT, Klukas O, Saenger W and Krauß N (2001) Three-dimensional structure of cyanobacterial photosystem I at 2.5 Å resolution. Nature 411: 909–917
Kargul J, Nield J and Barber J (2003) Three-dimensional reconstruction of a light-harvesting complex I–photosystem I (LHCI–PSI) supercomplex from the green alga Chlamydomonas reinhardtii –insights into light harvesting for PSI. J Biol Chem 278: 16135–16141
Kleima FJ, Gradinaru CC, Calkoen F, van Stokkum IHM, van Grondelle R and Van Amerongen H (1997) Energy transfer in LHCII monomers at 77 K studied by sub-picosecond transient absorption spectroscopy. Biochemistry 36: 15262–15268
Knoetzel J, Svendsen I and Simpson DJ (1992) Identification of the photosystem-I antenna polypeptides in barley –isolation of 3 pigment-binding antenna complexes. Eur J Biochem 206: 209–215
Knoetzel J, Bossmann B and Grimme LH (1998) Chlorina and viridis mutants of barley (Hordeum vulgare L.) allow assignment of long-wavelength chlorophyll forms to individual Lhca proteins of photosystem I in vivo. FEBS Lett 436: 339–342
Kuang T-Y, Argyroudi-Akoyunoglou JH, Nakatani HY, Watson J and Arntzen CJ (1984) The origin of the long-wavelength fluorescence emission band (77K) from photosystem I. Arch Biochem 235: 618–627
Köhlbrandt W, Wang DN and Fujiyoshi Y (1994) Atomic model of plant light-harvesting complex by electron crystallography. Nature 367: 614–621
Lam E, Ortiz W and Malkin R (1984a) Chlorophyll a/b proteins of photosystem I. FEBS Lett 168: 10–14
Lam E, Ortiz W, Mayfield S and Malkin R (1984b) Isolation and characterization of a light-harvesting chlorophyll a/b protein complex associated with photosystem I. Plant Physiol 74: 650–655
Lubben TH, Theg SM and Keegstra K (1988) Transport of proteins into chloroplasts. Photosynth Res 17: 173–194
Lunde C, Jensen PE, Haldrup A, Knoetzel J and Scheller HV (2000) The PSI-H subunit of photosystem I is essential for state transitions in plant photosynthesis. Nature 408: 613–615
Melkozernov AN and Blankenship RE (2003) Structural modeling of the Lhca4 subunit of LHCI-730 peripheral antenna in photosystem I based on similarity with LHCII. J Biol Chem 278: 44542–44551
Morosinotto T, Castelletti S, Breton J, Bassi R and Croce R (2002) Mutation analysis of Lhca1 antenna complex. Low energy absorption forms originate from pigment–pigment interactions. J Biol Chem 277: 36253–36261
Morosinotto T, Breton J, Bassi R and Croce R (2003) The nature of a chlorophyll ligand in Lhca proteins determines the far red fluorescence emission typical of photosystem I. J Biol Chem 278: 49223–49229
Mukerji I and Sauer K (1990) A spectroscopic study of a photosystem I antenna complex. Curr Res Photosynth Vol 2, 321–324
Mullet JE, Burke JJ and Arntzen CJ (1980a) A developmental study of photosystem I peripheral chlorophyll proteins. Plant Physiol 65: 823–827
Mullet JE, Burke JJ and Arntzen CJ (1980b) Chlorophyll proteins of photosystem I. Plant Physiol 65: 814–822
Palsson LO, Tjus SE, Andersson B and Gillbro T (1995) Ultrafast energy-transfer dynamics resolved in isolated spinach light-harvesting complex-I and the Lhc-I-730 subpopulation. Biochim Biophys Acta 1230: 1–9
Pascal A, Gastaldelli M, Ceoldo S, Bassi R and Robert B (2001) Pigment conformation and pigment–protein interactions in the reconstituted Lhcb4 antenna protein. FEBS Lett 492: 54–57
Plumley FG and Schmidt GW (1987) Reconstitution of chlorophyll a/b light-harvesting complexes: xanthophyll-dependent assembly and energy transfer. Proc Natl Acad Sci USA 84: 146–150
Polle JEW, Niyogi KK and Melis A (2001) Absence of lutein, violaxanthin and neoxanthin affects the functional chlorophyll antenna size of photosystem-II but not that of photosystem-I in the green alga Chlamydomonas reinhardtii. Plant Cell Physiol 42: 482–491
Preiss S, Peter GF, Anandan S and Thornber JP (1993) The multiple pigment–proteins of the photosystem-I antenna. Photochem Photobiol 57: 152–157
Remelli R, Varotto C, Sandona D, Croce R and Bassi R (1999) Chlorophyll binding to monomeric light-harvesting complex. A mutation analysis of chromophore-binding residues. J Biol Chem 274: 33510–33521
Roszak AW, Howard TD, Southall J, Gardiner AT, Law CJ, Isaacs NW and Cogdell RJ (2003) Crystal structure of the RC-LH1 core complex from Rhodopseudomonas palustris. Science 302: 1969–1972
Scheller HV, Jensen PE, Haldrup A, Lunde C and Knoetzel J (2001) Role of subunits in eukaryotic photosystem I. Biochim Biophys Acta 1507: 41–60
Schmid VHR, Cammarata KV, Bruns BU and Schmidt GW (1997) In vitro reconstitution of the photosystem I light-harvesting complex LHCI-730: heterodimerization is required for antenna pigment organization. Proc Natl Acad Sci 94: 7667–7672
Schmid VH, Thome P, Ruhle W, Paulsen H, Kuhlbrandt W and Rogl H (2001) Chlorophyll b is involved in long-wavelength spectral properties of light-harvesting complexes LHC I and LHC II. FEBS Lett 499: 27–31
Schmid VHR, Paulsen H and Rupprecht J (2002a) Identification of N- and C-terminal amino acids of Lhca1 and Lhca4 required for formation of the heterodimeric peripheral photosystem I antenna LHCI-730. Biochemistry 41: 9126–9131
Schmid VHR, Potthast S, Wiener M, Bergauer V, Paulsen H and Storf S (2002b) Pigment binding of photosystem I light-harvesting proteins. J Biol Chem 277: 37307–37314
Simpson DJ, Machold O, Hoyer-hansen G and von Wettstein D (1985) Chlorina mutants of barley (Hordeum vulgare L.). Carlsberg Res Commun 50: 223–238
Stauber EJ, Fink A, Markert C, Kruse O, Johanningmeier U and Hippler M (2003) Proteomics of Chlamydomonas reinhardtii light-harvesting proteins. Eukaryot Cell 2: 978–994
Tjus SE, Roobolboza M, Palsson LO and Andersson B (1995) Rapid isolation of photosystem-I chlorophyll-binding proteins by anion-exchange perfusion chromatography. Photosynth Res 45: 41–49
Vainstein A, Peterson CC and Thornber JP (1989) Light-harvesting pigment–proteins of photosystem I in maize. Subunit composition and biogenesis. J Biol Chem 264: 4058–4063
Van Amerongen H and van Grondelle R (2001) Understanding the energy transfer function of LHCII, the major light-harvesting complex of green plants. J Phys Chem B 105: 604–617
Wollman F-A and Bennoun P (1982) A new chlorophyll-protein complex related to photosystem I in Chlamydomonas reinhardtii. Biochim Biophys Acta 680: 352–360
Zhang H, Goodman HM and Jansson S (1997) Antisense inhibition of the photosystem I antenna protein Lhca4 in Arabidopsis thaliana. Plant Physiol 115: 1525–1531
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2006 Springer
About this chapter
Cite this chapter
Croce, R., Morosinotto, T., Bassi, R. (2006). LHCI: The Antenna Complex of Photosystem I in Plants and Green Algae. In: Golbeck, J.H. (eds) Photosystem I. Advances in Photosynthesis and Respiration, vol 24. Springer, Dordrecht. https://doi.org/10.1007/978-1-4020-4256-0_10
Download citation
DOI: https://doi.org/10.1007/978-1-4020-4256-0_10
Publisher Name: Springer, Dordrecht
Print ISBN: 978-1-4020-4255-3
Online ISBN: 978-1-4020-4256-0
eBook Packages: Biomedical and Life SciencesBiomedical and Life Sciences (R0)