Skip to main content

Experimental Tools to Study Regeneration in the Sea Anemone Nematostella vectensis

  • Protocol
  • First Online:
Developmental Biology of the Sea Urchin and Other Marine Invertebrates

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2219))

Abstract

Animal regeneration is a biological process leading to the reformation of injured or lost tissues/body parts. One of the most fascinating regenerative phenomena is the so-called whole-body regeneration, leading to the reformation of fully functional organisms within days after bisection. The sea anemone Nematostella vectensis is currently emerging as novel whole-body regeneration model. Here we describe the methods of inducing the regenerative process in this cnidarian as well as the fixation and staining protocols for morphological, molecular, and cellular analysis.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Subscribe and save

Springer+ Basic
$34.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or eBook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Protocol
USD 49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 109.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 139.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 249.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Similar content being viewed by others

References

  1. Bely AE, Nyberg KG (2010 Mar) Evolution of animal regeneration: re-emergence of a field. Trends Ecol Evol (Amst) 25(3):161–170

    Article  Google Scholar 

  2. Reitzel A, Burton P, Krone C, Finnerty J (2007) Comparison of developmental trajectories in the starlet sea anemone Nematostella vectensis: embryogenesis, regeneration, and two forms of asexual fission. Invertebr Biol 126(2):99–112

    Article  Google Scholar 

  3. Passamaneck YJ, Martindale MQ (2012) Cell proliferation is necessary for the regeneration of oral structures in the anthozoan cnidarian Nematostella vectensis. BMC Dev Biol 12(1):1–1

    Article  Google Scholar 

  4. Bossert PE, Dunn MP, Thomsen GH (2013) A staging system for the regeneration of a polyp from the aboral physa of the anthozoan cnidarian Nematostella vectensis. Dev Dyn 242:1320–1331

    Article  CAS  Google Scholar 

  5. Amiel AR, Johnston HT, Nedoncelle K, Warner JF, Ferreira S, Röttinger E (2015) Characterization of morphological and cellular events underlying oral regeneration in the sea anemone, Nematostella vectensis. Int J Mol Sci 16(12):28449–28471

    Article  CAS  Google Scholar 

  6. Zapata F, Goetz FE, Smith SA, Howison M, Siebert S, Church SH et al (2015) Phylogenomic analyses support traditional relationships within Cnidaria. PLoS One 10(10):e0139068

    Article  Google Scholar 

  7. Chang ES, Neuhof M, Rubinstein ND, Diamant A, Philippe H, Huchon D et al (2015) Genomic insights into the evolutionary origin of Myxozoa within Cnidaria. Proc Natl Acad Sci U S A 112(48):14912–14917

    Article  CAS  Google Scholar 

  8. Warner JF, Guerlais V, Amiel AR, Johnston H, Nedoncelle K, Röttinger E (2018) NvERTx: a gene expression database to compare embryogenesis and regeneration in the sea anemone Nematostella vectensis. Development 145(10):dev162867

    Article  Google Scholar 

  9. Hand C, Uhlinger KR (1992) The culture, sexual and asexual reproduction, and growth of the sea anemone Nematostella vectensis. Biol Bull 182(2):169–176

    Article  CAS  Google Scholar 

  10. Fritzenwanker JH, Technau U (2002) Induction of gametogenesis in the basal cnidarian Nematostella vectensis (Anthozoa). Dev Genes Evol 212(2):99–103

    Article  Google Scholar 

  11. Stefanik DJ, Friedman LE, Finnerty JR (2013) Collecting, rearing, spawning and inducing regeneration of the starlet sea anemone, Nematostella vectensis. Nat Protoc 8(5):916–923

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Eric Röttinger .

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 2021 Springer Science+Business Media, LLC, part of Springer Nature

About this protocol

Check for updates. Verify currency and authenticity via CrossMark

Cite this protocol

Amiel, A.R., Röttinger, E. (2021). Experimental Tools to Study Regeneration in the Sea Anemone Nematostella vectensis. In: Carroll, D.J., Stricker, S.A. (eds) Developmental Biology of the Sea Urchin and Other Marine Invertebrates. Methods in Molecular Biology, vol 2219. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0974-3_4

Download citation

  • DOI: https://doi.org/10.1007/978-1-0716-0974-3_4

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0973-6

  • Online ISBN: 978-1-0716-0974-3

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics