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Estimating Brain Permeability Using In Vitro Blood-Brain Barrier Models

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Permeability Barrier

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2367))

Abstract

The blood-brain barrier (BBB) is a vital biological interface that regulates transfer of different molecules between blood and brain and, therefore, maintains the homeostatic environment of the CNS. In order to perform high-throughput screening of therapeutics in drug discovery, specific properties of the BBB are investigated within in vitro BBB platforms. In this chapter, we detail the process and steps for the iPSC to BMEC and astrocyte differentiation as well as TEER and permeability measurement in Transwell platform of in vitro BBB model. Also, advanced microfluidic iPSCs-derived BMECs on chip and permeability measurement within this model have been elucidated.

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Author notes

  1. Saeideh Nozohouri and Behnam Noorani contributed equally to this work.

Authors and Affiliations

  1. Department of Pharmaceutical Sciences, School of Pharmacy, Texas Tech University Health Sciences Center, Amarillo, TX, USA

    Saeideh Nozohouri, Behnam Noorani, Abraham Al-Ahmad & Thomas J. Abbruscato

  2. Center for Blood-Brain Barrier Research, School of Pharmacy, Texas Tech University Health Sciences Center, Amarillo, TX, USA

    Saeideh Nozohouri, Behnam Noorani, Abraham Al-Ahmad & Thomas J. Abbruscato

Authors
  1. Saeideh Nozohouri
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  2. Behnam Noorani
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  3. Abraham Al-Ahmad
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  4. Thomas J. Abbruscato
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Correspondence to Thomas J. Abbruscato .

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  1. Ottawa, ON, Canada

    Kursad Turksen

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Nozohouri, S., Noorani, B., Al-Ahmad, A., Abbruscato, T.J. (2020). Estimating Brain Permeability Using In Vitro Blood-Brain Barrier Models. In: Turksen, K. (eds) Permeability Barrier. Methods in Molecular Biology, vol 2367. Humana, New York, NY. https://doi.org/10.1007/7651_2020_311

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  • DOI: https://doi.org/10.1007/7651_2020_311

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