Skip to main content

Production of Polyesters in Transgenic Plants

  • Chapter
  • First Online:
Biopolyesters

Part of the book series: Advances in Biochemical Engineering/Biotechnology ((ABE,volume 71))

Abstract

Polyhydroxyalkanoates (PHAs) are bacterial polyesters having the properties of biodegradable thermoplastics and elastomers. Synthesis of PHAs has been demonstrated in transgenic plants. Both polyhydroxybutyrate and the co-polymer poly(hydroxybutyrate-co-hydroxy-valerate) have been synthesized in the plastids of Arabidopsis thaliana and Brassica napus. Furthermore, a range of medium-chain-length PHAs has also been produced in plant pero-xisomes. Development of agricultural crops to produce PHA on a large scale and at low cost will be a challenging task requiring a coordinated and stable expression of several genes. Novel extraction methods designed to maximize the use of harvested plants for PHA, oil, carbohydrate, and feed production will be needed. In addition to their use as plastics, PHAs can also be used to modify fiber properties in plants such as cotton. Furthermore, PHA can be exploited as a novel tool to study the carbon flux through various metabolic pathways, such as the fatty acid β-oxidation cycle.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Subscribe and save

Springer+ Basic
$34.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or eBook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 84.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 109.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Similar content being viewed by others

References

  1. Goddijn OJM, Pen J (1995) Trends Biotechnol 13:379

    Article  CAS  Google Scholar 

  2. Anderson AJ, Dawes EA (1990) Microbiol Rev 54:450

    CAS  Google Scholar 

  3. Poirier Y, Nawrath C, Somerville C (1995) Nature Biotechnol 13:142

    Article  CAS  Google Scholar 

  4. Steinbüchel A (1991) Polyhydroxyalkanoic acids. In: Byrom D (ed) Novel biomaterials from biological sources. MacMillan, New York, p 123

    Google Scholar 

  5. Steinbüchel A, Schlegel HG (1991) Mol Microbiol 5:535

    Article  Google Scholar 

  6. Doi K (1990) Microbial polyesters. VCH, New York

    Google Scholar 

  7. Steinbüchel A, Füchtenbusch B (1998) Trends Biotechnol 16:419

    Article  Google Scholar 

  8. Poirier Y, Dennis DE, Klomparens K, Somerville C (1992) Science 256:520

    Article  CAS  Google Scholar 

  9. Poirier Y, Dennis DE, Klomparens K, Nawrath C, Somerville C (1992) FEMS Microbiol Rev 103:237

    Article  CAS  Google Scholar 

  10. Meyerowitz EM (1989) Cell 56:263

    Article  CAS  Google Scholar 

  11. Meyerowitz EM, Somerville (eds) (1995) Arabidopsis thaliana. CSH, Cold Spring Harbor

    Google Scholar 

  12. Peoples OP, Sinskey AJ (1989) J Biol. Chem 264:15, 293

    CAS  Google Scholar 

  13. Peoples OP, Sinskey AJ (1989) J Biol Chem 264:15, 298

    CAS  Google Scholar 

  14. Slater SC, Voige WH, Dennis DE (1988) J Bacteriol 170:4431

    CAS  Google Scholar 

  15. Schubert P, Steinbüchel A, Schlegel HG (1988) J Bacteriol 170:5837

    CAS  Google Scholar 

  16. de Koning (1995) Can J Microbiol 41(suppl 1):303

    Google Scholar 

  17. Poirier Y, Schechtman LA, Satkowski MM, Noda I, Somerville C (1995) Int J Biol Macromol 17:7

    Article  CAS  Google Scholar 

  18. Stuart ES, Lenz RW, Fuller RC (1995) Can J Microbiol 41(suppl 1):84

    Article  CAS  Google Scholar 

  19. Steinbüchel A, Aerts K, Babel W, Föllner C, Liebergesell M, Madkour MH, Mayer F, Pieper-Fürst U, Pries A, Valentin HE, Wieczorek K (1995) Can J Microbiol 41(suppl 1):94

    Google Scholar 

  20. Pieper-Fürst U, Madkour MH, Mayer F, Steinbüchel A (1994) J Bacteriol 176:4328

    Google Scholar 

  21. Pieper-Fürst U, Madkour MH, Mayer F, Steinbüchel A (1995) J Bacteriol 17:2513

    Google Scholar 

  22. Wieczorek R, Pries A, Steinbüchel A, Mayer F (1995) J Bacteriol 177:2425

    CAS  Google Scholar 

  23. Nawrath C, Poirier Y, Somerville CR (1994) Plastid targeting of the enzymes required for the production of polyhydroxybutyrate in higher plants. In: Doi Y, Fukuda K (eds) Biodegradable plastics polymers. Elsevier, Amsterdam, p 136

    Google Scholar 

  24. Nawrath C, Poirier Y, Somerville CR (1994) Proc Nat Acad Sci USA 91:12, 760

    Article  CAS  Google Scholar 

  25. Nakashita H, Arai Y, Yoshioka K, Fukui T, Doi Y, Usami R, Horikoshi K, Yamaguchi I (1999) Biosci Biotechnol Biochem 63:870

    Article  CAS  Google Scholar 

  26. Houmiel KL, Slater S, Broyles D, Casagrande L, Colburn S, Gonzalez K, Mitsky TA, Reiser SE, Shah D, Taylor NB, Tran M, Valentin HE, Gruys KJ (1999) Planta 209:547

    Article  CAS  Google Scholar 

  27. Slater S, Mitsky TA, Houmiel KL, Hao M, Reiser SE, Taylor NB, Tran M, Valentin HE, Rodriguez DJ, Stone DA, Padgette SR, Kishore G, Gruys KJ (1999) Nature Biotechnol 17:1011

    Article  CAS  Google Scholar 

  28. Lehninger AL, Nelson DL, Cox MM (1993) Principles of biochemistry. Worth Pub, New York

    Google Scholar 

  29. Schweiger G, Buckel W (1984) FEBS Lett 171:79

    Article  CAS  Google Scholar 

  30. Williams DR, Anderson AJ, Dawes EA, Ewing DF (1994) Appl Microbiol Biotechnol 40:717

    Article  CAS  Google Scholar 

  31. Gerbling H, Gerhart B (1989) Plant Physiol 91:1387

    CAS  Google Scholar 

  32. Camp PJ, Randall DD (1985) Plant Physiol 77:571

    CAS  Google Scholar 

  33. LaRossa RA, Van Dyk T, Smulski DR (1987) J Bacteriol 169:1372

    CAS  Google Scholar 

  34. Van Dyk TK, LaRossa RA (1987) Mol Gen Genet 207:435

    Article  Google Scholar 

  35. Rhodes D, Hogan AL, Deal L, Jamieson GC, Haworth P (1987) Plant Physiol 84:775

    Article  CAS  Google Scholar 

  36. Shaner DL, Singh BK (1993) Plant Physiol 103:1221

    CAS  Google Scholar 

  37. Singh BK, Shaner DL (1992) Carbon flow through branched-chain amino acid biosynthetic pathway: lessons from acetohydroxy acid synthase inhibitors. In: Singh BK, Flores HE, Shannon JC (eds) Biosynthesis and molecular regulation of amino acids in plants. American Society of Plant Physiologists, Maryland, p 354

    Google Scholar 

  38. Calhoun DH, Rimerman RA, Hatfield GW (1973) J Biol Chem 25:3511

    Google Scholar 

  39. Eisenstein E, Yu HD, Fisher KE, Iacuzio DA, Ducote DR, Schwarz RP (1995) Biochemistry 34:9403

    Article  CAS  Google Scholar 

  40. Slater S, Houmiel KL, Tran M, Mitsky TA, Taylor NB, Padgette SR, Gruys KJ (1998) J Bact 180:197, 941

    Google Scholar 

  41. Valentin HE, Broyles DL, Casagrande LA, Colburn SM, Creely WL, DeLaquil PA, Felton HM, Gonzalez KA, Houmiel KL, Lutke K, Mahadeo DA, Mitsky TA, Padgette SR, Reiser SE, Slater S, Stark DM, Stock RT, Stone DA, Taylor NB, Thorne GM, Tran M, Gruys K (1999) Int J Biol Macromol 25:30, 341

    Article  Google Scholar 

  42. Steinbüchel A, Valentin HE (1995) FEMS Microbiol Lett 128:219

    Article  Google Scholar 

  43. De Koning GJM, Van Bilesen HMM, Lemstra PJ, Hazenberg W, Withold B, Preusting H, Van der Galiën JG, Schirmer A, Jendrossek D (1994) Polymer 35:2090

    Article  Google Scholar 

  44. Lageveen RG, Huisman GW, Preusting H, Ketelaar P, Eggink G, Witholt B (1988) Appl Environ Microbiol 54:2924

    CAS  Google Scholar 

  45. Schulz H (1991) Biochim Biophys Acta 1081:109

    CAS  Google Scholar 

  46. Gerhard B (1993) Catabolism of fatty acid acids. In: Moore TS (ed) Lipid metabolism in plants. CRS Press, Bâton Rouge, p 527

    Google Scholar 

  47. Fukui T, Shiomi N, Doi Y (1998) J Bacteriol 180:667

    CAS  Google Scholar 

  48. Haywood GW, Anderson AJ, Ewing DF, Dawes EA (1990) Appl Environ Microbiol 56:3354

    CAS  Google Scholar 

  49. Timm A, Steinbüchel A (1990) Appl Environ Microbiol 56:3360

    CAS  Google Scholar 

  50. Huijberts GNM, Eggink G, de Waard P, Huisman GW, Witholt B (1992) Appl Environ Microbiol 58:536

    CAS  Google Scholar 

  51. Saito Y, Doi Y (1993) Int J Biol Macromol 15:287

    Article  CAS  Google Scholar 

  52. Huijbert GNM, de Rijk TC, de Waard P, Eggink G (1994) J Bacteriol 176:1661

    Google Scholar 

  53. Rehm BHA, Krüger N, Steinbüchel A (1998) J Biol Chem 273:24, 044

    Article  CAS  Google Scholar 

  54. Mittendorf V, Robertson EJ, Leech RM, Krüger N, Steinbüchel A, Poirier Y (1998) Proc Natl Acad Sci USA 95:13, 397

    Article  CAS  Google Scholar 

  55. Mittendorf V, Krüger N, Steinbüchel A, Poirier Y (1998) Biosynthesis of medium-chainlength polyhydroxyalkanoates in transgenic Arabidopsis plants expressing the PhaC1 and PhaC2 synthases from Pseudomonas aeruginosa. In: Steinbüchel A (ed) Biochemical principles and mechanisms of biosynthesis and biodegradation of polymers. Wiley-VCH, Weinheim, p 368

    Google Scholar 

  56. Preisig-Müller R, Gühnemann-Schäfer KG, Kindl H (1994) J Biol Chem 269:20, 475

    Google Scholar 

  57. Engeland KK, Kindl H (1991) Eur J Biochem 200:171

    Article  CAS  Google Scholar 

  58. Mittendorf V, Bongcam V, Allenbach L, Coullerez G, Martini N, Poirier Y (1999) Plant J 20:45–55

    Article  CAS  Google Scholar 

  59. McConn M, Browse J (1996) Plant Cell 8:403

    Article  CAS  Google Scholar 

  60. Voelker TA, Worrell AC, Anderson L, Bleibaum J, Fan C, Hawkins DJ, Radke SE, Davies HM (1992) Science 257:72

    Article  CAS  Google Scholar 

  61. Jones A, Davies HM, Voelker TA (1995) Plant Cell 7:359

    Article  CAS  Google Scholar 

  62. Facciotti MT, Yuan L (1998) Fett/Lipid 4/5:167

    Article  Google Scholar 

  63. Eccleston VS, Cranmer AM, Voelker TA, Ohlrogge JB (1996) Planta 198:46

    Article  CAS  Google Scholar 

  64. Hooks MA, Fleming Y, Larson TR, Graham IA (1999) Planta 207:385

    Article  CAS  Google Scholar 

  65. Martini N, Schell J, Abbadi A, Spener F, Töpfer R (1999) Vorträge für Pflanzenzüchtung 45:133

    Google Scholar 

  66. John ME, Keller G (1996) Proc Natl Acad Sci USA 93:12, 768

    Article  CAS  Google Scholar 

  67. John ME (1997) Crit Rev Biotechnol 17:185

    Article  CAS  Google Scholar 

  68. Jendrossek D, Schirmer A, Schlegel HG (1996) Appl Microbiol Biotechnol 46:451

    Article  CAS  Google Scholar 

  69. Van de Loo FJ, Broun P, Turner S, Somerville C (1995) Proc Natl Acad Sci USA 92:6743

    Article  Google Scholar 

  70. Lee M, Lenman M, Banas A, Bafor M, Singh S, Schweizer M, Nilsson R, Liljenberg C, Dahlqvist A, Gummeson PO, Sjodahl S, Green A, Stymne S (1998) Science 280:915

    Article  CAS  Google Scholar 

  71. Byrom D (1987) Trends Biotechnol 5:246

    Article  CAS  Google Scholar 

  72. Berger E, Ramsay BA, Ramsay JA, Chavarie C, Braunegg G (1989) Biotechnol Tech 3:227

    Article  CAS  Google Scholar 

  73. Ramsay JA, Berger E, Ramsay BA, Chavarie C (1990) Biotechnol Tech 4:221

    Article  CAS  Google Scholar 

  74. Martin DP, Peoples OP, Williams SF (1997) PCT application WO 97/15, 681

    Google Scholar 

  75. Noda I (1997) PCT application WO 97/07, 230

    Google Scholar 

  76. Kurdikar DL, Strauser FE, Solodar AJ, Paster MD, Asrar J (1998) PCT application WO 98/46, 782

    Google Scholar 

  77. Noda I (1998) US Pat 5, 821, 299

    Google Scholar 

  78. Kurdikar DL, Strauser FE, Solodar AJ, Paster MD, Asrar J (1998) PCT application WO 98/46, 783

    Google Scholar 

  79. Seebach D, Roggo S, Zimmermann J (1987) Biological-chemical preparation of 3-hydroxycarboxylic acids and their use in EPC-synthesis. In: Bartmann W, Sharpless KB (eds) Stereochemistry of organic and bioorganic transformations. VCH Verlagsgesellschaft, Weinheim, p 85

    Google Scholar 

  80. Liddell (1997) PCT application WO 97/17, 459

    Google Scholar 

  81. Noda I (1998) US Pat 5, 849, 854

    Google Scholar 

  82. Noda I (1999) US Pat 5, 899, 339

    Google Scholar 

  83. Whistler RL, BeMiller JN, Paschall EF (eds) (1984) Starch: chemistry and technology. Academic Press, Orlando

    Google Scholar 

  84. Röbbelen G, Downey RK, Ashri A (eds) (1989) Oil crops of the world. McGraw-Hill, New York

    Google Scholar 

  85. Moore JW (1992) Modern Plastics 69:58

    Google Scholar 

  86. Chemical Market Reporter (1998) 254

    Google Scholar 

  87. Page WJ (1997) Waste sources for polyhydroxyalkanoate production. In: Eggink G, Steinbüchel A, Poirier Y, Witholt B (eds) 1996 International Symposium on Bacterial Polyhydroxyalkanoates. NRC Research Press, Ottawa, p 56

    Google Scholar 

  88. Lee SY, Choi J, Chang HN (1997) Process development and economic evaluation for the production of polyhydroxyalkanoates by Alcaligenes eutrophus. In: Eggink G, Steinbüchel A, Poirier Y, Witholt B (eds) 1996 International Symposium on Bacterial Polyhydroxyalkanoates. NRC Research Press, Ottawa, p 127

    Google Scholar 

  89. de Koning G, Kellerhals M, van Meurs C, Witholt B (1997) A process for the production of bacterial medium-chain-length poly[(R)-3-hydroxyalkanoates]: reviewing the status quo. In: Eggink G, Steinbüchel A, Poirier Y, Witholt B (eds) 1996 International Symposium on Bacterial Polyhydroxyalkanoates. NRC Research Press, Ottawa, p 137

    Google Scholar 

  90. Lee SY (1996) Trends Biotechnol 14:431

    Article  CAS  Google Scholar 

  91. de Koning G, Kellerhals M, van Meurs C, Witholt B (1997) Bioprocess Eng 17:15

    Article  Google Scholar 

  92. Meyer P (1998) Stabilities and instabilities in transgene expression. In: Lindsey K (ed) Transgenic plant research. Harwood Academic Publishers, Amsterdam, p 263

    Google Scholar 

  93. Baulcombe DC (1999) Cur Opin Plant Biol 2:109

    Article  CAS  Google Scholar 

  94. Ulker B, Allen GC, Thompson WF, Spiker S, Weissinger AK (1999) Plant J 18:25395. Vain P, Worland B, Kohli A, Snape JW, Christou P, Allen GC, Thompson WF (1999) Plant J 18:233

    Article  Google Scholar 

  95. Vaucheret H, Beclin C, Elmayan T, Feuerbach F, Godon C, Morel JB, Mourrain P, Palauqui JC, Vernhettes S (1998) Plant J 16:651

    Article  CAS  Google Scholar 

  96. Elmayan T, Balzergue S, Beon F, Bourdon V, Daubremet J, Guenet Y, Mourrain P, Palauqui JC, Vernhettes S, Vialle T, Wostrikoff K, Vaucheret H (1998) Plant Cell 10:1747

    Article  CAS  Google Scholar 

  97. Kerr R (1998) Science 281:1128

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 2001 Springer-Verlag Berlin Heidelberg

About this chapter

Cite this chapter

Poirier, Y. (2001). Production of Polyesters in Transgenic Plants. In: Babel, W., Steinbüchel, A. (eds) Biopolyesters. Advances in Biochemical Engineering/Biotechnology, vol 71. Springer, Berlin, Heidelberg. https://doi.org/10.1007/3-540-40021-4_7

Download citation

  • DOI: https://doi.org/10.1007/3-540-40021-4_7

  • Received:

  • Published:

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-540-41141-3

  • Online ISBN: 978-3-540-40021-9

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics