Abstract
Seed dormancy is an emerging problem related to germination which is common in many species of ornamental trees and flowers. Poor seed germination and subsequently poor field establishment are a common phenomenon at adverse conditions of environment. The most important problems faced are the heterogneity and lack of suitable conditions in soil that causes decrease in germination percent. Priming is a water-based technique that consents metabolic processes necessary for enhancing germination rate and seed quality by managing the temperature and seed moisture content in which the seed is taken through the first biochemical processes within the initial stages of germination but preventing the seed transition towards full germination. This is a successful way through which plants would be able to complete their growth on or before the stresses arrive (Subedi KD, Ma BL. Agron J 97(1):211–218, 2005). Seed priming technique has been practised in many countries including India, Pakistan, China and Australia, and more than thousand trials had been conducted to evaluate the performance of priming in a variety of crops. The principle of seed priming is to minimise the period of emergence and to protect seed from environmental stresses during critical phase of seedling establishment to synchronise emergence which lead to uniform establishment and improved yield. It reduces the effect of salinity on the morphological parameter of the plants. Various priming techniques, like osmopriming, biopriming, halopriming, thermopriming, hydropriming, hormonal priming and solid matrix priming, give favourable result in seeds of ornamental flowers as well as trees. This technique has been successfully carried out in flower crops like balsam, coneflower, cosmos, gladiolus, pansy, marigold, periwinkle, rudbeckia, salvia, snapdragon and zinnia and trees like cassia, cypress, senegal, eucalyptus, fig, teak, pine, almond, tamarind, oak, karanj, khejri, siris, subabul, kapok, gulmohar, kachnar, etc.
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Keywords
Establishment of crop is the primary importance for optimising horticultural production. Every year, mankind awaits for the miraculous transmogrification of seeds into plants and again into seeds. Poor seed germination and subsequently poor field establishment are a common phenomenon at adverse conditions of environment. It has been reported that the major difficulty to high yield and production of crop plants is due to the lack of synchronised crop establishment and adverse weather and soil conditions (Mwale et al. 2003).
Seed dormancy is another problem related to germination which is common in many species. It is an adaptation that allows a species to regulate the timing of germination for seeds in a population. Some species use environmental cues (such as drought, rainfall or temperatures) to integrate germination for most seeds at a particular time of the year. Temperature, moisture, air and light conditions are the most important factors for seed germination. Minimum temperature is the lowest temperature at which seeds can germinate effectively (Nleya et al. 2005), and the maximum is the highest temperature at which seeds can germinate. Slight change in this temperature can damage seeds or make them go into the dormancy condition. At optimal temperatures, germination is rapid and uniform. Seeds need correct moisture to initiate internal processes leading up to germination. Osmotic adjustment or priming of seeds prior to sowing is known as a potent way to increase germination and emergence rate in some species with stepwise seed development (Sivritepe 2000).
Seed priming technique has been practised in many countries including India, Pakistan, China and Australia, and more than thousand trials had been conducted to evaluate the performance of priming in a variety of crops. The need for increased seed quality has become a priority necessary to tackle the current demand for high standards of seeds in the agricultural market. Achieving rapid and uniform seedling emergence is a key point for crop performance since slow germination rates frequently expose plantlets to adverse environmental conditions and soilborne diseases (Osburn and Schroth 1989). Priming is a water-based technique that consents metabolic processes necessary for enhancing germination rate and seed quality by managing the temperature and seed moisture content in which the seed is taken through the first biochemical processes within the initial stages of germination but preventing the seed transition towards full germination. This is the way through which plants would be able to complete their growth on or before the stresses arrive (Subedi and Ma 2005).
The above graph shows about the standard germination process and seed priming process in the factory and field condition. The green line corresponds to a standard germination process. During Phase I (imbibition), seeds take up water sufficiently under suitable availability of temperature and moisture. During Phase II (activation), the biochemical processes are activated and will eventually start the germination in Phase III (germination) where roots and hypocotyls emerge from the seeds. During priming, the seed invloves the activation processes followed by drying, before root can emerge from the seeds. Once conditions (temperature and moisture) are appropriate in the field, Phase III can continue, and germination occurs in a much shorter time (Hasegawa 2016).
The purpose of seed priming is to minimise the period of emergence and to protect seed from environmental stresses during critical phase of seedling establishment to synchronise emergence which lead to uniform establishment and improved yield. It reduces the effect of salinity on the morphological parameter of the plants. One of the priming techniques called osmopriming is a commercially used technique for improving seed germination and vigour. It controls imbibition of seeds to start the initial germination process followed by seed drying up to its original weight. Apart from germination process, seed priming also helps in growth and flowering of the crops. Various seed priming techniques like hormonal priming and chemopriming for enhancing flowering and growth of plant are commercially practised in- or on-farm basis. Where, on farm seed priming is energy intensive, high technology seed priming, seed hardening or seed conditioning process are available to farmers to help them in high input temperate agriculture and horticulture. (Harris et al. 2001).
The priming treatments which enhance seed germination include hydropriming (Afzal et al. 2004), biopriming, halopriming, solid matrix priming, chemopriming, thermopriming, osmopriming and hormonal priming (Afzal et al. 2006).
1 Physiological and Biochemical Aspects of Priming
A successful application of seed management technique depends upon the type of test, method of application, selection of crop, initial performance of the crop, selection of chemical, its concentration, duration of treatment and the purpose of implication. Priming method in seed management techniques is proven very essential factor for enhancing quality issues, germination rate, establishment, etc. Priming can inverse some of the ageing-induced deteriorative events, resulting in improved seed performance (Taylor et al. 1998). It has shown an immense effect to activate different processes related to cell cycle and to induce synthesis of nuclear DNA in radial tip cells in tomato (Liu et al. 1997).
Long duration seed storage caused a decline in the level of protein content which may cause oxidation of the amino acids, due to the increase in the respiratory activity and advance in the degradation process of the stored seeds. Seed deterioration causes loss of membrane integrity, changes in enzymatic activities and declines in protein and nucleic acid synthesis and lesions in DNA (McDonald 1999). Priming with 30% PEG for 24 h resulted in increase in the activity of superoxide dismutase (SOD) and peroxidise (POD) which enhance the intensity of respiration of plant and cause an increase in vigourity in germination (Jie et al. 2002). Priming is also thought to increase the activity of many enzymes involved in metabolism of carbohydrates (α- and β-amylases), proteins (proteases) and lipids mobilisation (isocitrate lyase) that are implicated in the stored reserves mobilisation (Varier et al. 2010; Di Girolamo and Barbanti 2012). These enzymes are vital in the breakdown of macromolecules for the development and growth of the embryo that ultimately result in early and higher seedling emergence (Farooq et al. 2006a, b; Varier et al. 2010). There are reports that priming facilitates the repair of chromosomal damage (Sivritepe and Dourado 1995), permits early DNA replication and repair, increases RNA and de novo protein synthesis and reduces the leakage of metabolites (McDonald 2000; Farooq et al. 2007a, b; Manonmani et al. 2014; Paparella et al. 2015). Thus, total seed protein, POD, PPO, RNA and de novo protein synthesis were increased significantly by seed priming. Among the various processes of priming, osmopriming may enhance rapid seed germination by reducing mechanical hindrance on the germinating embryo (Toorop et al. 1998).
2 Seed Priming Techniques
Seed priming includes various techniques which influences growth, establishment and germination of seeds and also influences yield of the crop. Techniques include the following.
2.1 Hydropriming (Drum Priming)
A major cause of poor establishment and low crop yield in agricultural areas is the lack of moisture content that reduces the ability of seedling to further emerge and growth. Hydropriming is a technique for enhancing germination without the emergence of the radicle and plumule which involves soaking of seeds in a priming agent solution followed by drying even if seeds are infected with pathogens (McDonald 1999). In hydropriming, distilled water plays a vital role for imbibition up to 10–20% (Pill 1995). It results into uncontrolled water uptake, since the process depends on seed affinity to water and the main critical point is to find and maintain optimal temperature and humidity conditions to avoid radicle protrusion (Taylor et al. 1998). Another limiting factor of hydropriming is the lack of homogeneous seed hydration which can lead to uneven germination (McDonald 2000). The main variant of hydropriming also called drum priming, patented by Rowse (1991) in which a drum that contains seeds is connected with a boiler generating vapour. The vapour condenses into liquid water inside the drum. The machine measures the increase in seed relative mass during the treatment. The time and volume of water required to complete seed rehydration are strictly controlled to reach gradual and even seed imbibition (Warren and Bennett 1997). Hydropriming is the most ancient type of priming, since the benefits of this pre-sowing treatment have been known for a long time; however, it is now applied less frequently in comparison with other methods.
2.2 Biopriming
Biopriming is a new technique of seed treatment that assimilates biological (inoculation of seed with beneficial organism to protect seed) and physiological aspects (seed hydration) of disease control. To respond to the negative effects of pathogens, biopriming uses beneficial microorganisms to protect against pathogens and enhance plant growth. Biological seed treatments for control of seed and seedling diseases offer the grower an alternative to chemical fungicides. Storage and application conditions are more critical than with chemical seed protectants and differential reaction to hosts, and environmental conditions may cause biological seed treatments to have a narrower spectrum of use than chemicals. Conversely, some biocontrol agents applied as seed dressers are capable of colonising the rhizosphere, potentially providing benefits to the plant beyond the seedling emergence stage (Nancy et al. 1997). Seed treatment with biocontrol agents along with priming agents may serve as an important means of managing many of the soil- and seed-borne diseases, the process often known as ‘biopriming’. It involves coating seed with a bacterial biocontrol agent such as Pseudomonas aureofaciens AB254 and hydrating for 20 h under warm (23 °C) conditions in moist vermiculite or on moist germination blotters in a self-sealing plastic bag. The seeds are removed before radical emergence. The bacterial biocontrol agent may multiply substantially on seed during biopriming (Callan et al. 1990). Biopriming seed treatments can provide a high level of protection against root rot diseases of crop plants which was generally equal or superior to the control provided with fungicide seed treatment. So, it could be suggested that biopriming (combined treatments between seed priming and seed coating with biocontrol agents) may be safely used commercially as substitute for traditional fungicide seed treatments for controlling seed- and soilborne plant pathogens.
2.3 Halopriming
Halopriming is one of the methods of priming practices that includes salts like CaCl2, CaSO4 and NaCl in such a way that the pregermination metabolic activities start preventing radical protrusion followed by drying seeds to the original moisture level (McDonald 2000). In this method, the seeds are immersed in different salt solutions which facilitate the process of seed germination and subsequent seedling emergence even under adverse environmental conditions. Seeds treated with NaCl concentrations should be in a tolerable limit. Early initiation of metabolic activities and reserve breakdown and mobilisation might be the reason for faster germination in such type of primed seeds. Khan et al. (2009) reported salt priming induced salinity tolerance of hot pepper at seedling stage, wherein seed priming improved significantly the germination percentage and index, vigour index, plumule and radical length and dry weight of seedling as compared to the non-primed seeds (control). Improved stress tolerance of primed plants is thought to rise from the activation of cellular defence response due to halopriming (Beckers and Conrath 2007). This has been substantiated by reports on better antioxidant system in primed plants (Afzal et al. 2006) on exposure to stress. Conrath et al. (2006) proposed that halopriming could involve accumulation of signalling proteins or transcription factors. Halopriming is a simple and cheap agrotechnique and found suitable to be recommended to the farmers owing to better synchrony of emergence and crop stand under various conditions of environment (Sedghi et al. 2010).
2.4 Solid Matrix Priming
Solid matrix priming (SMP) is similar to osmotic priming that allows the seed to attain a threshold moisture content and pre-germinative metabolic activity but preventing radicle emergence. However, it has the advantages of allowing aeration, incorporation of biological agents to combat soilborne pathogens and improved ease of handling (Taylor et al. 1988; Wang et al. 1998). The matrix comprises of finely divided non-plant pathogenic water holding solid, which may be carbonaceous substance, preferably a lignateous solid which has a large eqilibrium water potential (ψ) and preferably has an osmotic potential component which is atleast about 90% or greater than 95% of the total water potential. Such materials include coal, especially soft coal, lignateous shale such as the leonardite shale, sold as Agro-Lig, and sphagnum moss. The matrix material when containing the water to prime the seeds must be sufficiently friable, non-clumping, etc., so that it can be mechanically separated from the treated seeds after treatment without damage to the seeds when required. The process of SMP includes the admixture of a predetermined amount of solid matrix material and a predetermined amount of water and the mixture allowed to stand preferably in a container which allows entry of air but reduces evaporative losses, resulting in sufficient amount of moisture level in seeds. Matrices can be readily used in tree seed nursery operations.
2.5 Thermopriming
Seed treatments carried out at various intervals of time before sowing are known as thermopriming. Information exists to specify that seeds germinate better under alternating temperature conditions compared to a constant daily temperature (Felippe 1980; Shin et al. 2006; Markovskaya et al. 2007). Changing temperature can break the dormancy of seed easily. This technique has been widely adapted to improve germination efficiency under adverse climate reducing thermo-inhibition of seed germination (Huang et al. 2002). Pre-sowing seed treatments with alternating daily temperature regimes have resulted in enhanced plant development, increased cold and/or frost resistance and higher plant productivity in cucumber and melon (Markovskaya et al. 2007). Small changes in ambient temperature can regulate flowering time via a thermosensory pathway (Franklin 2009). It was shown that cold treatment at the seedling stage can modulate the flowering of some ornamental plants (Runkle et al. 1999; Garner and Armitage 2008). Although high temperature condition has been used in some species, resulting pregermination especially for plants adapted to warm climates (Khalil and Rasmussen 1983). Seeds of white spruce (Picea glauca L.), lettuce, etc. that were primed with combinations with other treatments resulted in beneficial effects on germination parameters (Liu et al. 2013; Ashraf and Foolad 2005). It has been suggested that priming is responsible to repair the age-related cellular and subcellular damage of low-vigour seeds that may accumulate during seed development (Bray 1995). Wang et al. (2003) reported that both thermo- and hydro-primed seeds showed significant increase in germination performance. The resultant effect of priming depends on the method used and time of treatment.
2.6 Osmopriming
Osmopriming, also known as osmotic conditioning, is a widespread pre-sowing priming procedure which involves treatments of seeds with osmotic solutions at low water potential facilitating the control of water uptake into the seeds. The main goal of osmopriming is to limit the reactive oxidative species, i.e. ROS-mediated oxidative injury through insufficient water absorption. Thus, the water potential of the osmotic agent used is a crucial parameter during the priming process (Heydecker and Coolbear 1977; Taylor et al. 1998). Priming with PEG provides beneficial conditions for bacterial growth due to poor aeration (Parera and Cantliffe 1994). It shows some disadvantage when used in bulk, due to high costs and extremely high viscosity which limits oxygen transfer within the solution. Hence, research has to be done for choosing the correct chemical and its optimum dose for a crop. It is difficult to manage huge quantities of wet primed seed especially under hot tropical climate condition, while in temperate areas, maintaining the priming temperature is crucial. Some of the osmotica (osmotic compounds used for osmopriming) that can be used include potassium nitrate, potassium dihydrogen orthophosphate, dipotassium hydrogen orthophosphate, calcium chloride, zinc sulphate, borax, magnesium chloride, manganese sulphate, sodium chloride, sodium sulphate and organic compounds, viz. agrosan, cycocel, citric, furamic, succinic, malic acids, purines, pyrimidines, caffeine, uracil, xanthine and uridine diphosphate (De Chandra 1999).
2.7 Hormonal Priming
Hormone pretreatment is a commonly used priming approach to improve seed germination in stressful conditions (Atici et al. 2003; Gratao et al. 2005; Jisha et al. 2013; Masood et al. 2012; Hu et al. 2013). Hormonal priming in general consists of treatment of seeds with chemicals like growth regulators, sodium hypochlorite (NaOCl) or hydrochloric acid (HCl), natural substances and agrichemicals (e.g. fungicides, pesticides). It has reduced the severity of the effect of salinity, but the amelioration was found maximum due to the application of 50 ppm salicylic acid and 50 ppm ascorbic acid treatments and gives satisfied results on seedling growth, fresh and dry weights under non-saline and saline conditions, whereas hormonal priming with ABA was not effective in some of grass family crops (Afzal et al. 2006). In pepper (Capscum annum L.), Khan et al. (2009) showed that pretreatment with acetylsalicylic acid and salicylic acid resulted in greater uniformity of germination and establishment of seedlings under high salinity. In addition to these chemicals, ethylene was used to minimise the effect of high temperatures on seed germination of lettuce (Nascimento 2004, Nascimento et al. 2005).
3 Seed Priming in Ornamental Flower Crops
3.1 Balsam
Response of hormonal priming on flower quality, growth and germination rate of balsam was observed according to the basis of germination rate dry weight and shoot and root length. GA3 at 10 ppm strikingly enhanced the germination percentage and speed of germination of balsam. The germination was higher in large seeds (grade A) which might be due to more supply of food material to the growing embryo. However, GA3 at 30 ppm increased the length of shoot and root, dry weight and fresh weight of seedlings reported by Singh and Karki (2003).
3.2 Coneflower
Osmotic priming in polyethylene glycol (PEG) or matrix priming in expanded vermiculite had greater rate, synchrony and germination percentage at 20 °C than non-primed seeds of coneflower (Echinacea purpurea). Osmotic or matrix priming for 10 days at −0.4 MPa and 15 °C resulted in higher germination rate and germination percentage than short duration of exposure (5 days) or lower (−1.5 MPa) water potential. Seedling emergence rate, synchrony and percentage from osmotically or matrically primed seeds were similar in both cool (23–27 °C day) and warm (35–40 °C) glasshouse regimes. Emergence was faster in primed than from non-primed seeds in both regimes. Emergence percentage was higher (80%) from primed seeds than from non-primed seeds (50%) in the cool regime, but emergence synchrony was unaffected. Moistened vermiculite substituted for PEG solution as a priming medium for purple coneflower seeds benefits to seed germination or seedling emergence followed by priming (−0.4 MPa, 15 °C, 10 days of darkness) in these media (Pill et al. 1994).
3.3 Cosmos
In cosmos (Cosmos bipinnatus), hormonal priming plays a main role in enhancing flowering and quality of flowers. The triazoles, which include paclobutrazol (PB) and uniconazole, are more potent and persistent than most other growth retardants. PB is used most commonly in commercial practice, but non-uniform plant size can result from non-uniform spray application. Soaking of cosmos seeds to 1000 ppm PB reduced seedling shoot height but also reduced seedling emergence percentage (Pill and Gunter 2001). Seed treatment with PB also eliminates conventional fungicide seed coating treatment since the triazoles themselves are potent fungicides (Fletcher and Gilley 2000).
3.4 Fir
Seeds of true firs, including pacific silver fir (Abies amabilis), subalpine fir (A. lasiocarpa) and noble fir (A. procera), exhibit deep dormancy at maturity. To break the dormancy termination, seeds generally require prolonged moist-chilling condition (i.e. 3–4 months or longer) (Edwards 1981, 1986; Leadem 1986; Tanaka and Edwards 1986; Edwards 1996). In some cases, germination can be impaired by seed-borne pathogens where some of the seedlots have a high proportion of empty seed (Kolotelo 1998). This seed dormancy can be broken down with solid matrix priming when seeds are existed in most chilling temperature. Agro-Lig Greens Grade (humic acids with particle sizes between 0.212 and 1.29 mm), sand (particle size 1.29 mm), peat moss and sphagnum moss are used in matrix priming that help in breaking dormancy in seeds of Abies spp. and early germination in seeds with high seedling establishment (Ma et al. 2003).
3.5 Gladiolus
Gladiolus alatus is a prominent bulbous cut flower that sometimes gives less productivity due to low-quality seed, inadequate seedbed preparation, late sowing, poor sowing technique, inadequate soil moisture, adverse soil properties and high temperatures. Seed priming has been successfully demonstrated in this crop to improve germination and emergence in seeds. It is the enhancement of physiological and biochemical events in seeds during interruption of germination by low osmotic potential and negligible matric potential of the imbibing medium. Hydro-primed seeds compared to KNO3-treated seeds (osmo-primed) were allowed to imbibe water for a longer time. Seeds treated with 0.25% KNO3 attained the maximum (66.67%) level of germination followed by 60% in 0.75% KNO3 (60%) and distilled water. After 30 days, the germination percentage was recorded maximum with treatment of 0.25% KNO3, 0.75% KNO3 and distilled water (83.33%) followed by non-priming (63%) and 0.5% KNO3 (60%)-treated seeds (Mushtaq et al. 2012).
3.6 Meadow Fescue
Priming is highly useful for seeds under stress condition. NaCl priming (halopriming) and hydropriming on germination and early growth of Festuca arundinacea and Festuca ovina seeds were studied under salinity condition. It was observed that NaCl priming with concentrations of 15 and 45 dS/m in Festuca arundinacea seeds and NaCl priming with concentration 45 dS/m in Festuca ovina seeds had the highest performance of improved seed in both species at germination and early growth stages under salinity stress (Shakarami et al. 2011).
3.7 Pansy
Temperature stress is one of the most important factors that affect the growth and development of seeds of pansy (Viola tricolor). Thermo-inhibited seeds fail to germinate in high temperature but can germinate when temperature is reduced which may result in thermo-death of the plant which is mostly seen in pansy. So in such condition, there is a need for priming of seeds that may induce the germination rate by improving seed quality. Dorna et al. (2014) reported that hydropriming, halopriming and osmopriming gave significant effect on germination of pansy seeds. Osmopriming seeds in polyethylene glycol (PEG) solutions of −1.25 and −1.5 MPa osmotic potential at 15 °C and in PEG solution of −1.0 MPa osmotic potential at 20 °C increased the percentage of germinating seeds significantly at 30 °C. Osmopriming seeds, in all combinations used, improved the percentage of germinating seeds significantly at 35 °C. The best result was observed when seeds were primed in PEG solution of −1.0 MPa osmotic potential at 20 °C. At higher temperature hydropriming seeds in volume of water 600 μl H2O g/seed for 3 days at 15 °C and osmopriming at 20 °C positively affected the germination rate. After osmopriming of seeds at 15 °C and after osmopriming in PEG solution of −1.0 MPa osmotic potential at 20 °C, the percentage of ungerminated seeds was lower than treatment control. Both halopriming at 20 °C and osmopriming, regardless of temperature and osmotic potential of PEG, improved significantly the uniformity of germination at 30 °C compared with untreated seeds. It was also observed that osmopriming followed by halopriming improved the speed of germination in pansy at 20 °C to the largest extent. Moisture content was also increased due to priming, but drying of seeds after priming affects the moisture content (Suleman et al. 2011).
3.8 Periwinkle
Seed priming (biopriming) increases antioxidant activity and seedling vigour in seeds of periwinkle (Catharanthus roseus). Seeds were treated with diazotrophs, i.e. Azospirillum and Azotobacter as separate treatments for 30 min. The germination percentage was calculated from 8 days after sowing (DAS) to 12 DAS. There was a significant increase in germination rate and non-significant in dry matter content. There was a significant increase in SOD, POX and CAT activities under Azotobacter and Azospirillum treatments and also an increase in the germination percentage, root length, shoot length and vigour index of the C. roseus (Karthikey et al. 2007).
3.9 Pot Marigold
Calendula officinalis, commonly known as pot marigold, was positively affected by seed priming method that influences the germination percentage and quality of seeds. Hydro- and hormonal priming give paramount results in seeds of pot marigold. Water or distilled water is used as hydrating agent in hydropriming in which seeds are needed to be soaked for few hours. As a result, sufficient water gets imbibed into the cell wall of marigold seeds that enhances the germination and further growth. In hormonal priming, GA3 results better among all the growth regulators in relation to germination rate, flowering and total sugars (Karimi and Varyani 2016), but increase in concentration adversely affects the germination rate. However, GA3 in addition to KNO3 results profuse seedling establishment, shooting, rooting, maximum root length and vigour index in seeds of pot marigold. It was also noticed that the catalase activity increased significantly with GA3 application, while enzyme activity was higher in the distilled water and KNO3 treatments compared with the untreated seeds.
3.10 Rudbeckia
Rudbeckia fulgida, also known as black-eyed Susan, gloriosa daisy and orange coneflower, is a herbaceous perennial plant. Seed germination in this plant is variable with variable species. Osmopriming is a process of controlled imbibition by the seeds using different concentrations of osmotic solution containing polyethylene glycol (PEG). Accumulation of solutes and enzymatic activation during controlled seed imbibition (Bewley and Black 1978) contributed to the increased radicle emergence rate in primed seeds (Fay et al. 1994).
3.11 Safflower
In safflower (Carthamus tinctorius), hydropriming has an immense impact in increasing number of plants/m2, capitula/plant, grains/capitulum, etc. In a field experiment, hydropriming of safflower (Carthamus tinctorius) seed for 12 h resulted in higher number of plants/m2, capitula/plant, grains/capitulum, 1000 seed weight, grain yield and oil content compared to untreated seed (Bastia et al. 1999).
3.12 Salvia
The germination of Salvia officinalis L. (sage) seeds is a problem of great concern that may be overcome by employing the seed priming techniques. An effect of hydropriming positively affects the germination and seedling growth in sage (Salvia officinalis). Priming helps in increasing final germination percentage, improving germination rate, accelerating the synchronised seed germination, vigorous seedling establishment and stimulating vegetative growth and crop yield of many crops. Significantly, higher germination percentage and rate of germination observed in hydro-primed seeds as compared to non-primed seeds indicated a positive effect of seed priming in synchronising the seed germination process (Dastanpoor et al. 2013).
3.13 Snapdragon
Seed priming affects the germination growth and flowering of many flowers among which snapdragon (Antirrhinum majus) is one of them. There is an imperative need to work on priming techniques of these flower seeds because of the higher price and, moreover, the seeds of F1 hybrids are difficult to germinate. The effect of biopriming influenced the germination character, shooting, rooting and flowering of snapdragon using T. harzianum and B. subtilis as bioprimers (Bhargava et al. 2015). Hormonal priming also has positive effect on biochemical changes in seeds that improves membrane integrity and metabolism of seed cell wall as well as synthesis of proteins (globulins and cruciferin) in comparison to non-primed seeds (Varier et al. 2010; Rao et al. 2009).
3.14 Sunflower
Biopriming in sunflower gave the utmost result for controlling fungal disease called Alternaria blight, the most important disease, and estimates yield loss up to 80%. Integration of chemicals, plant extracts and biotic agents along with priming agents for managing plant diseases has been considered as a novel approach, as it requires low amounts of chemicals, reducing the cost of control and pollution hazards while causing minimum interference with biological equilibrium (Papavizas 1973). Biopriming of sunflower seeds with P. fluorescens in the form of jelly can be used as an alternative method to seed treatment with chemicals (hexaconazole as foliar spray) which is eco-friendly and avoids possible residue problems.
3.15 Zinnia
It was found that Alternaria zinniae seems the most important fungal seed-borne pathogen of zinnia plants (Zinnia elegans), causing spotting of the petals, foliage and stems and rotting of the roots (Dimock and Osborn 1943; Richardson 1990; Łacicowa et al. 1991; Palacios et al. 1991; Wu and Yang, 1992). Łacicowa et al. (1991) reported that zinnia seeds produced in Poland were commonly infested with A. alternata, A. zinniae, Botrytis cinerea, Fusarium spp. and Penicillium spp. The influence of osmopriming on germination, vigour and location of pathogenic and saprotrophic fungi in zinnia seeds was studied in different varieties of zinnia (Szopinska and Tylkowska 2003) at 20 °C at 45% for 24 h that were sterilised with 1% NaOCl for 10 min at 20 °C at darkness. Disinfection of primed seeds lowered the germination capacity and increased the number of deformed seedlings in variety Jowita, Red man and Talia seeds. Seed priming considerably affected the speed of germination, regardless of sodium hypochlorite (NaOCl) treatment.
4 Seed Priming in Trees
Most of the tree species face the problem of extinction due to over-exploitation (Onochie 1990). Seeds of forest trees have problematic seed germination due to adverse soil and environment condition. Various salt contents in soil impaired germination of these seeds when available in excess amount, i.e. when the salt concentration increased up to 0.1%. Various salts like sodium, calcium and magnesium are the most common that contribute to salinity. High levels of fertilisation also contribute to salt accumulation and can be significant in agricultural situations (Treshow 1970). Sometimes, the lack of availability of moisture in seeds affects germination process adversely. Seed priming is an alternative way to avoid this type of problem. Some examples of seed priming of forest trees are mentioned below.
4.1 Baobab Tree or Senegal Tree
Baobab tree or senegal tree is scientifically known as Adansonia digitata, a deciduous tree. Most of the seeds of Adansonia fail to germinate as their propagation is adversely affected by seed coat dormancy which leads to poor growth potential. Sometimes the seeds are unable to germinate in natural condition. Osmopriming and thermopriming help in overcoming the dormancy problem in seeds of Adansonia. Hot water, cold water and H2SO4 are used for osmopriming and useful in enhancing germination percentage (70%) (Falemara et al. 2013). Different concentration of H2SO4 is used with variable time periods. Seeds’ emergence is very fast (10 days) when treated with cold water.
4.2 Cassia
Cassias are ornamental plants of great beauty including more than 1000 species. Seed production is irregular with low germination percentage in natural conditions due to the integument impermeability. The vigour of stored seeds can be increased with priming, with a decrease in costs and reduction in number of collections (Vertucci 1989). Osmopriming by taking PEG with different water potentials is a helpful method for increasing germination tendency in seeds of cassia. Lowering in osmotic potential results in delaying in radicle emergence (Tarquis and Bradford 1992). Osmo-primed seeds are immediately used without drying or dehydrating as it enhances the germination ability of seeds (Heydecker and Wainwright 1976).
4.3 Cedar
Cedrus libani, called as Taurus cedar, is now an extinct species and difficult to grow in a large population. The temperature had the strong effect on the germination of C. libani seeds (Bewley and Black 1994; Schmidt 2000). The seeds of this tree exhibit seed dormancy that may be corrected by different pretreatment methods or priming methods. The seeds exhibit better germination performance at lower constant temperatures. Rise in germination temperature may develop secondary dormancy in seeds (Khan and Samimy 1982).
4.4 Cypress
Arizona cypress (Cupressus arizonica) and medite cypress (Cupressus sempervirens) are very important forest tree species for multiple purposes in forestry because of their ability to grow in adverse environments such as calcareous, clayish, dry and poor soils (Gallis et al. 2007). Seeds can germinate even in drought condition by using PEG-8000 as osmopriming agent. The germination percentage decreased in seeds of Arizona cypress with decreased water potential.
4.5 Eucalyptus
Eucalyptus has about 700 species and is commercially used for timber and pulpwood for paper and other purposes. Poor germination and seedling establishment are regarded as common problem in eucalyptus which can be corrected by pregermination treatments. Seed size is the most important factor that affects germination in eucalyptus seeds. Spring-germinated seedlings have long growing period and attain maximum size with advanced establishment. Thus, larger seeds are more likely to survive in winter frosts as their susceptible growing tips are better adapted to cold-induced photoinhibition due to more advanced foliar pigment development (Close et al. 2000).
4.6 Fig
Seeds are the important planting material for propagation in Ficus spp. through which genetically different plants can be developed. It is also easily propagated through rooting and cuttings. Osmopriming, hydropriming and hormonal priming are useful treatment in early establishment of seedlings in Ficus. KNO3, GA3 and water are used for priming purpose. Early germination due to the priming effect of GA3 results into longest radicle, which helps in early establishment of new seedling to produce maximum food material with the help of photosynthesis that resulted into the maximum survival of seedlings (Rawat et al. 2010)
4.7 Gamhar or White Teak
Gmelina arborea (gamhar) is a fast-growing deciduous tree used for furniture, carriages, sports and musical instruments as its steady timber is moderately resistant to termites. Seeds of Gmelina arborea (white teak or gamhar) don’t have any dormancy and can easily germinate. Hydropriming in seeds may influence the germination tendency and increase the seedling emergence. Also KMnO4 (0.2 M) treatment increases the germination percentage when seeds are treated with different interval of time duration.
4.8 Gulmohar
Delonix regia also known as gulmohar exhibits seed dormancy. For breaking the seed dormancy in seeds of Delonix, hormonal priming is efficiently useful by using different plant hormones, i.e. ABA, BAP, GA and IBA. Hot water treatment is also useful that tends to give the germination percentage of 95% in seeds of gulmohar. ABA does not have significant effect in breaking the dormancy. Gibberellic acid will promote the endosperm breakdown and the growth of the embryo that results in the elongation of radicle cells and cause a rupture in the micropyle, and the seed dormancy will be terminated by radicle protrusion from the seed coat. The role of temperature in breaking the dormancy to influence germination can be noticed due to the erratic nature of the germination which terms for the standardisation of both the temperature of the water and duration of cooling as suggested by Owonubi and Otegbeye (2004).
4.9 Henkel’s Yellowwood
This ornamental tree is dioecious in nature and is used for ornamental purpose. Generally, this henkel’s yellowwood tree (Podocarpus henkelii) is propagated through seeds. Fleshy fruit that surrounds the seed is removed as this inhibits the germination. Removal of epicuticular wax, the epidermis or the entire epimatium leads to rapid water uptake and germination (Dodd et al. 1989). Seeds stored in shade have more moisture content and reserve more starch through biochemical process. Lipids are the major reserve materials in seeds of Podocarpus henkelii followed by proteins, and these embryonic reserves are sufficient for early seedling establishment (Nabanyumya et al. 2015). Soaking seeds more than once a day also breaks dormancy in seeds, resulting in maximum germination percentage.
4.10 Whistling Pine
The whistling pine (Casuarina equisetifolia) is used as a windbreak in agroforestry system and also for many household uses. The wood is resistant to decomposition in soil or saltwater and is often used as roundwood for making piles, poles and fences. This is accentuated as the successful propagation of any Casuarina spp. by a vegetative method is very limited even with IBA hormone application (Mahmood and Possuswam 1980; Pinyopusarerk and Bolan 1990). Different concentrations of growth hormones like GA3 and ABA, acid substances like sulphuric acid and chemical like NaNO3 are helpful in promoting germination and growth in this tree. The waxy and hard seed coat in seeds of Casuarina prevents it to germinate which has been broken with the use of concentrated H2SO4 by several workers (Mayer and Poljakoff-Mayber 1963; Ballard 1973; Gill and Bamidele 1981; Etejere et al. 1982; Eze and Orole 1987).
4.11 Ivory Coast Almond
Terminalia ivorensis, a tropical deciduous tree, thrives in a wide range of soil and is used as multipurpose trees along with having medicinal uses. Seed germination of tropical species is influenced by several biological factors such as seed viability, seed size (Barrera and Nobel 2003) and plant growth regulators through which acts as germination stimulator (Chen et al. 2008). Seeds are very sensitive to temperature stress as it influences the germination process, and high temperature prevents the elongation of radicle and shoot by inhibiting the synthesis of protein and nucleic acid (Sivaramakrishnan et al. 1990). Soaking seeds of T. Chebula (Horitaki) enhances germination rate and vigour index (Hossain et al. 2005). Gibberellic acid can overcome the dormancy in seeds of Terminalia spp. by inducing rapid and uniform germination due to deficit in endogenous gibberellic acids (Asomaning et al. 2011). The exogenously applied gibberellic acids help in modifying the influence of cytokinins on transport across membranes and are thus able to initiate the biochemical processes necessary for germination process (Chen et al. 2008).
4.12 Kachnar
Bauhinia spp. (mountain ebony) are flowering plants and propagated through seeds and cuttings. This tree has been prioritised as one of the trees for conservation to enhance its contribution to health and livelihood of communities. Various methods are there to break the dormancy in the seeds of this tree that promotes its cultivation and successful regeneration. Mainly germination in kachnar depends on the size of the seeds as larger seeds germinate more percentage than smaller one. Soaking in boiled water makes the seed coats permeable to water and water gets imbibed into the cell wall which results in swelling of the seed as water cools. Recommendation of hot water must be applied judiciously without killing the seeds with excessive heating (Phartyal et al. 2005). Chemical stimulators like KNO3 enhance seed germination by creating a balance between hormonal ratios in the seed and reducing the growth retarding substances like abscisic acid (ABA).
4.13 Karanj (Indian Beech)
The use of biologically active products like biomanures and biofertilisers for the production of quality planting material helps in preventing microbial inoculation of bacteria, algae and fungi in karanj (Revathi et al. 2013). Biopriming with liquid Azospirillum and Phosphobacterium in different concentrations improves germination, seedling vigour and storability of Pongamia pinnata seeds by reducing microbial infections. An increase in the seed germination might be due to the increased cytokinin production which actively involved in cell division (Suma et al. 2014) and production of growth-regulating substances like auxin, GA and cytokinin (Kucey 1988) when seeds are encapsulated with biofertilisers.
4.14 Khejri
Prosopis cineraria (khejri) is a flowering tree of which seeds sometimes show dormancy effect which can be lessened by priming methods. Fulvic acid that is extracted from compost is beneficial in enhancing germination tendency in seeds of this tree. Fulvic acids are beneficial to increase the permeability of seed coat and plant cell membranes and enhance enzymatic activity of the root system leading to increased root proliferation (Trevisan et al. 2010). Application of CaCO3 can cause buffering capacity in soil that affects nutrient availability to plants, while sulfuric acid treatment has conventionally been used for breaking the dormancy and softens the seed coat through which the radicle can easily protrude.
4.15 Oak
Himalayan oak trees (Quercus glauca) have irregular fructification, and consumption of seeds by animals (Troup 1921) and loss of viability during storage for extended periods (Chalupa 1995) have overrated the problem of regeneration (artificial and natural). Propagation (clonal propagation) through stem cuttings is difficult in most of the oak species and has not been much successful in these species. Even the seeds’ weight is also affected by germination percentage. Seed coat acts as a mechanical barrier in germination of oak seeds which prevents radicle emergence and is corrected by several scarification methods and priming treatments (Alderete-Chavez et al. 2011). The KNO3 plays an osmotic role on water uptake with a nutritional effect on protein synthesis. KNO3 is used for growth regeneration and also as germination-stimulating substance in many species (Ozturk et al. 1994).
4.16 Pine
It is the most widely distributed tree used for timber purposes and for making boxes, paper pulp and temporary electric poles. Seed germination in pine (Pinus kesiya) is a common problem which depends on the moisture level and substrate pH level in seeds (Verma and Tondon 2010). Hydropriming increases the imbibition rate and moisture level in seeds which enhances the germination rate. Available soil moisture also influences germination and early seedling survival. Influence in germination in pine is due to leaching of growth inhibitors and increase in endogenous level of growth regulators when seeds scarified or subjected to chilling treatment.
4.17 Siris
Albizia lebbeck (siris) has special place among all the forest crops. Despite its importance, the species is becoming scarce due to deep seed dormancy. Seed priming is a method used for producing numerous number of planting material by overcoming its seed coat-imposed dormancy (Baskin and Baskin 1998). Seeds are often used for propagation in Siris which is a cheapest method of propagation in many species of this tree. Its hard seed coat makes the germination process difficult in nursery and/or out in the fields. Sulphuric acid, hot water and gibberellic acid are used for breaking dormancy in this species (Alderete-Chavez et al. 2011; Baskin and Baskin 1974). The disintegration of the seed coat increases the imbibition and subsequent germination in seeds when treated with sulphuric acid (Egley 1989).
4.18 Kapok
The silk cotton tree (kapok), Ceiba pentandra, is a fast-growing and emergent tropical forest tree species which can grow up to a height of 60 m (Gribel et al. 1999). It is one of the extinct species among forest trees, and seeds of this tree are also limitedly available. It is pollinated by hummingbirds (Gribel et al. 1999; Toledo 1977) and non-flying primate mammals, particularly by Saimiris ciureus (squirrel monkey), Cebus paella (capuchin) and Ateles paniscus (spider monkey) (Janson et al. 1981). Seed soaking in seeds of silk cotton tree significantly influences the germination process. But heavy rainfall may limit the process of germination and fails to break the seed dormancy.
4.19 Subabul
This subabul tree (Leucaena leucocephala) is also called ‘miracle tree’ due to its multipurpose nature (Ssenku et al. 2017). Germination capacity in seeds of subabul can increase by different priming or pretreatment methods. NaCl concentration in soil shows a marked effect on the germination. With the increase in salinity, the germination initiation in this species is delayed and gets ceased (Rafiq et al. 2006). The reason behind this is that the osmotic pressure of the soil solution increases with increase in salt concentration that results in the prevention of uptake of water. It also reduces the germination energy of seeds.
4.20 Tamarind
Tamarind (Tamarindus indica) has been recognised for its potential nitrogen-fixing nature (Okoro et al. 1986). Seeds in tamarind do not have self-capacity to germinate due to the lack of the factors required for breaking dormancy. Hot water treatment and sulphuric acid affect the germination rate, and seed germination increased with increasing water temperature and soaking period.
4.21 Teak
Teak (Tectona grandis) is a tropical tree species, hardy and deciduous in nature, is valued for its durability and water resistance and is used for boat building, exterior construction, veneer, furniture, carving and other small wood decorative uses. Germination of the seeds involves pretreatment to remove dormancy arising from the thick pericarp. Pretreatment involves alternate drying and wetting of the seeds. Saline solution also affect the germination ability of seeds in teak, as increased salinity results in decreased germination ability of seeds and delayed rate of germination. This is due to the complex nature of salts that present in the solution for germination which may be sometimes having toxic effect in germination.
4.22 Wigandia
Wigandia caracasana, or Caracus wigandia, is a species of evergreen ornamental plant having purple flowers in large clusters. Sometimes, seed burial can improve the chances of establishment of seedlings through breaking the dormancy. Priming consists of a regulated hydration process that permits enhancement of some metabolic processes through the osmotic solutions or water. Burial enhanced germination process and favoured uniform and rapid germination of W. urens seeds (Gonzalez-Zertuche et al. 2001). This enhancement in germination is due to the increased protein synthesis in burial seeds as compared to control seeds. Burial with priming treatment in seeds increases the germination percentage, seedling growth and breaking dormancy than the priming treatment alone.
4.23 Willow Tree
Willow tree (Salix babylonica ), also called sallows, is a deciduous tree as well shrub. Seeds are viable for a short period of time. Priming methods are useful in enhancing germination and growth of dormant seeds. Thermopriming gives efficient result in germination up to 100%. Seeds of willow tree results in better germination rate in lower temperature regimes (Young and Clements 2003).
5 Conclusion
There is a huge gap between the number of seeds sown and availability of stocky seedlings in any ornamental flower crops and several important tree species. The most important problems faced are the heterogneity and lack of suitable conditions in soil that cause decrease in germination percentage. Heterogeneous emergence, unbalanced seedling growth and competition for environmental resources such as light, nutrients and water subsequently make difference in biomass and performance of plants. Priming is helpful in reducing the risk of poor stand establishment under a wide range of environmental conditions. The purpose of these treatments is to shorten the emergence and to protect seed from biotic and abiotic factors during critical phase of seedling establishment so as to synchronise emergence, which lead to uniform stand and improved yield.
References
Afzal I, Aslam N, Mahmood F, Hameed A, Irfan S, Ahmad G (2004) Enhancement of germination and emergence of canola seeds by different priming techniques. Caderno Pesquisasérie Biol 16(1):19–34
Afzal I, Basra SM, Farooq M, Nawaz A (2006) Alleviation of salinity stress in spring wheat by hormonal priming with ABA, salicylic acid and ascorbic acid. Int J Agric Biol 8(1):23–28
Alderete-Chavez A, Cruz-Landero NDL, Guerra-Santos JJ, Guevara E, Gelabert R (2011) Promotion of germination of Bauhinia divaricata L. seeds by effects of chemical scarification. Res J Seed Sci 4:51–57
Ashraf M, Foolad MR (2005) Pre-sowing seed treatment-A shotgun approach to improve germination, plant growth, and crop yield under saline and non-saline conditions. Adv Agron 88:223–271
Asomaning JM, Sacandle M, Olympus NS (2011) Germination response of Terminalia superba Enl. And diels seed on the 2-way grant’s thermogradient plate. Res J Seed Sci 4:28–39
Atici O, Agar G, Battal P (2003) Interaction between endogenous plant hormones and alphamylase in germinating chickpeas seeds under cadmium exposure. Fresenius Environ Bull 12:781–785
Ballard LA (1973) Physical barriers to germination. Seed Sci Technol 1: 285–295.
Baskin JM, Baskin CC (1974) Breaking dormancy in seeds of Isanthus branchiathus (Labiatae) with gibberellic acid. Phyton 32:159–165
Baskin JM, Baskin CC (1998) Ecology, biogeography and evolution of dormancy and germination. Academic, San Diego, p 666
Bastia DK, Rout AK, Mohanty SK, Prusty AM (1999) Effect of sowing date sowing methods and seed soaking on yield and oil content of rainfed safflower grown in Kalahandi. Orissa Indian J Agron 44:621–623
Beckers GJ, Conrath U (2007) Priming for stress resistance: from the lab to the field. Curr Opin Plant Biol 10(4):425–431
Bewley JD, Black M (1978) Mobilization of reserves. In: Physiology and Biochemistry of seeds in relation to germination. Springer, Berlin/Heidelberg, pp 177–244
Bewley JD, Black M (1994) Seeds: physiology of development and germination, 2nd edn. Plenum Press, New York
Bhargava B, Gupta YC, Dhiman SR, Sharma P (2015) Effect of seed priming on germination, growth and flowering of snapdragon (Antirrhinum majus L.). Natl Acad Sci Lett 38(1):81–85
Bray CM (1995) Biochemical processes during the osmopriming of seeds. Seed development and germination. Marcel Dekker, New York, pp 767–789
Callan NW, Mathre DE, Miller JB (1990) Bio-priming seed treatment for biological control of Pythium ultimum pre-emergence damping-off in sh2 sweet corn. Plant Dis 74:368–372
Chalupa V (1995) Somatic embryogenesis in oak (Quercus spp.). In: Jain SM, Gupta PK, Newton RJ (eds) Somatic embryogenesis in woody plants, vol 2. KulwerAcademic Publishers, Dordrecht, pp 67–87
Chen SY, Kuo SR, Chien CT (2008) Roles of gibberellins and abscisic acid in dormancy and germination of red bayberry (Myrica rubra) seeds. Tree Physiol 28(9):1431–1439
Close DC, Beadle CL, Brown PH, Holz GK (2000) Cold-induced photoinhibition affects establishment of Eucalyptus nitens (Deane and Maiden) Maiden and Eucalyptus globulus Labill. Trees-Struct Funct 15(1):32–41
Conrath U, Beckers GJM, Flors V, Garcia-Agustin P, Jakab G (2006) Priming: getting ready for battle. Mol Plant-Microbe Interact 19:1062–1071
Dastanpoor N, Fahimi H, Shariati M, Davazdahemami S, Hashemi SMM (2013) Effects of hydropriming on seed germination and seedling growth in sage (Salvia officinalis L.). Afr J Biotechnol 12(11):1223–1228
De Chandra G (1999) Fundamentals of agronomy. Oxford and IBH Publishing Company, New-Delhi
De la Barrera E, Nobel PS (2003) Physiological ecology of seed germination for the columnar cactus Stenocereus queretaroensis. J Arid Environ 53(3):297–306
Di Girolamo G, Barbanti L (2012) Treatment conditions and biochemical processes influencing seed priming effectiveness. Ital J Agron 7(2):25
Dimock AW, Osborn JH (1943) An Alternaria disease of zinnia. Phytopathology 33:372–381
Dodd MC, Staden JV, Smith MT (1989) Seed germination in Podocarpus henkelii: an ultrastructural and biochemical study. Ann Bot 64(5):569–579
Dorna H, Li W, Szopinska D (2014) The effect of priming on germination and vigour of pansy (Viola× Wittrockiana Gams.) seeds. Acta Sci Pol Hortoru 13(6):15–29
Edwards DGW (1981) A new prechilling method for true fir seeds. Proceeding: Combined Meetg. Intermountain Nurseryman’s Assoc. and Western For. Nurs. Assoc., Idaho. USDA 109:58–66
Edwards DGW (1996) The stratification-re-dry technique with special reference to true fir seeds. In: Landis TD, D.B. South, Technical Coordinators (eds) National proceedings, forest conservation nursery associations, General Technical Report PNW-GTR-389. U.S.D.A. Forest Service, Pacific Northwest Research Station, Portland, pp 172–182
Egley GH (1989) Water-impermeable seed coverings as barriers to germination. In: Recent advances in the development and germination of seeds. Springer, Boston, pp 207–223
Etejere EO, Fawole MO, Sani A (1982) Studies on the seed germination of Parkia clappertoniana. Turrialba 32(2):181–185
Eze JMO, Orole BC (1987) Germination of the seeds of Prosopis africana. Niger J For 17:12–16
Falemara BC, Nwadike C, Obashola EO (2013) Germination response of baobab seeds (Adansonia digitata L.) as influenced by three pretreatment techniques. In: Forest industry in a dynamic global environment. Proceeding: 35th annual conference of Forestry Association of Nigeria, Sokoto, Sokoto state, pp 44–55
Farooq M, Basra SMA, Hafeez K (2006a) Seed invigoration by osmohardening in coarse and fi ne rice (Oryza sativa L.). Seed Sci Technol 34:181–187
Farooq M, Basra SMA, Wahid A (2006b) Priming of field sown rice seed germination, seedling establishment, allometry and yield. Plant Growth Regul 49:285–294
Farooq M, Basra SMA, Khan MB (2007a) Seed priming improves growth of nursery seedlings and yield of transplanted rice. Arch Agron Soil Sci 53:1–12
Farooq M, Basra SMA, Wahid A (2007b) Improving the performance of transplanted rice by seed priming. Plant Growth Regul 51:129–137
Fay AM, Bennett MA, Still SM (1994) Osmotic seed priming of Rudbeckia fulgida improves germination and expands germination range. Hortscience 29(8):868–870
Felippe GM (1980) Germination of the light-sensitive seeds of and : effects of temperature. New Phytol 84(3):439–448
Fletcher RA, Gilley A (2000) Triazoles as plant growth regulators and stress protectants. Hortic Rev 24:55–138
Franklin KA (2009) Light and temperature signal crosstalk in plant development. Curr Opin Plant Biol 12(1):63–68
Gallis AT, Doulis AG, Papageorgiou AC (2007) Variability of cortex terpene composition in Cupressus sempervirens L. provenances grown in Crete, Greece. Silvae Genet 56(6):294–299
Garner JM, Armitage AM (2008) Cooling and long-day lighting influences growth and flowering of Phlox paniculata L. ‘Ice Cap’ used for cut flowers. Hortscience 43(3):707–709
Gill LS, Bamidele JF (1981) Seed morphology, germination and cytology of three savanna trees of Nigeria. Niger J For 2:16–23
Gonzalez-Zertuche L, Vazquez-Yanes C, Gamboa A, Sanchez-Coronado ME, Aguilera P, Orozco-Segovia A (2001) Natural priming of Wigandia urens seeds during burial: effects on germination, growth and protein expression. Seed Sci Res 11(1):27–34
Gratao Pl, Polle A, Lea PJ, Azevedo R (2005) Making the life of heavy metal-stressed plants a little easier. Funct Plant Biol 32:481–494
Gribel R, Gibbs PE, Queiróz AL (1999) Flowering phenology and pollination biology of Ceiba pentandra (Bombacaceae) in Central Amazonia. J Trop Ecol 15(3):247–263
Harris D, Pathan AK, Gothkar P, Joshi A, Chivasa W, Nyamudeza P (2001) On-farm seed priming: using participatory methods to revive and refine a key technology. Agric Syst 69(1):151–164
Hasegawa S (2016) What is seed priming?, Germains Seed Technology. https://germains.com/what-is-seed-priming
Heydecker W, Wainwright H (1976) More rapid and uniform germination of Cyclamen persicum L. Sci Hortic 5(2):183–189
Heydecker W, Coolbear P (1977) Seed treatments for improved performance survey and attempted prognosis. Seed Sci Technol 5:353–425
Hossain MA, Arefin MK, Khan BM, Rahman MA (2005) Effects of seed treatments on germination and seedling growth attributes of Horitaki (Terminalia chebula Retz.) in the nursery. Res J Agric Biol Sci 1(2):135–141
Hu YF, Zhou G, Na XF, Yang L, Nan WB, Zhang Y, Li J, Bi Y (2013) Cadmium interferes with maintenance of auxin homeostasis in Arabidopsis seedlings. J Plant Physiol 170:965–975
Huang YM, Wang HH, Chen KH (2002) Application of seed priming treatments in spinach (Spinacia oleracea L.) production. J Chin Soc Hort Sci 48:117–123
Janson CH, Terborgh J, Emmons LH (1981) Non-Flying Mammals as Pollinating Agents in the Amazonian Forest. Biotropica 13(2):1
Jie LIU, Gong she L, Dong mei Q, Fang fang L, Enhua W (2002) Effect of PEG on germination and active oxygen metabolism in wildrye (Leymuschinensis) seeds. Acta Prataculturae Sin 11(1):59–64
Jisha K, Vijayakumari K, Puthur J (2013) Seed priming for abiotic stress tolerance: an overview. Acta Physiol Plant 35:1381–1396
Karimi M, Varyani M (2016) Role of priming technique in germination parameters of calendula (Calendula officinalis L.) seeds. J Agric Sci 61(3):215–226
Karthikeyan B, Jaleel CA, Gopi R, Deiveekasundaram M (2007) Science letters: alterations in seedling vigour and antioxidant enzyme activities in Catharanthus roseus under seed priming with native diazotrophs. J Zhejiang Sci 8(7):453
Khalil MAK, Rasmussen RA (1983) Sources, sinks and seasonal cycles of atmospheric methane. J Geophys Res Oceans 88(9):5131–5144
Khan AA, Samimy EC (1982) Hormones in relation to primary and secondary seed dormancy. In: Khan AA (ed) Physiology and biochemistry of seed development, dormancy and germination. Elsevier, Amsterdam, pp 203–241
Khan HA, Ayub CM, Pervez MA, Bilal RM, Shahid MA, Ziaf K (2009) Effect of seed priming with NaCl on salinity tolerance of hot pepper (Capsicum annuum L.) at seedling stage. Soil Environ 28(1):81–87
Kolotelo D (1998) Abies seeds problems. Proceedings: 1995, 1996, 1997 Forest Nursery Association of British Columbia Meetings, B.C. Ministry of Forests, Surrey, B.C., Canada., pp 122–130
Kucey RMN (1988) Alteration of size of wheat root systems and nitrogen fixing bacteria measured under field conditions. Can J Microbiol 34:735–739
Lacicowa B, Kiecana I, Pięta D (1991) Mikroflora materiału siewnego roślin ozdobnych. I. Mikroflora materiału siewnego cynii (Zinnia elegans L.)i groszku pachnącego (Lathyrus odoratus L.). Inst Sadown Kwiac Ser B 16:109–116
Leadem CL (1986) Stratification of Abies amabilis seeds. Can J For Res 16:755–760
Liu Y, Hilhorst HW, Groot SP, Bino RJ (1997) Amounts of nuclear DNA and internal morphology of gibberellin-and abscisic acid-deficient tomato (Lycopersicon esculentum Mill.) seeds during maturation, imbibition and germination. Ann Bot 79(2):161–168
Liu Y, Kermode A, El-Kashaby YO (2013) The role of moist-chilling and thermo-priming on the germination characteristics of white spruce (Picea glauca) seed. Seed Sci Tech 41:1–15
Ma Y, Feurtado JA, Kermode AR (2003) Effect of solid matrix priming during moist chilling on dormancy breakage and germination of seeds of four fir species. New For 25(1):67–81
Mahmood AM, Possuswam PK (1980) Propagation of Casuarina junghuhniana by planting shoots and root suckers. Indian For 106(4):298–299
Manonmani V, Begum MAJ, Jayanthi M (2014) Halo priming of seeds. Res J Seed Sci 7(1):1–13
Markovskaya EF, Sysoeva MI, Sherudilo EG, Topchieva LV (2007) Differential gene expression in cucumber plants in response to brief daily cold treatments. Russ J Plant Physiol 54(5):607–611
Masood A, Iqbal N, Khan N (2012) Role of ethylene in alleviation of cadmium-induced capacity inhibition by sulphur in mustard. Plant Cell Environ 35:524–533
Mayer AM, Poljakoff-Mayber A (1963) The germination of seeds. Pergamon Press, London, pp 31–94
McDonald MB (1999) Seed deterioration: physiology, repair and assessment. Seed Sci Technol 27(1):177–237
McDonald MB (2000) Seed priming. In: Black M, Bewley JD (eds) Seed technology and its biological basis. Sheffield Academic Press, Sheffield, pp 287–325
Mushtaq S, Hafiz IA, Hasan SZU, Arif M, Shehzad MA, Rafique R, Iqbal MS (2012) Evaluation of seed priming on germination of Gladiolus alatus. Afr J Biotechnol 11(52):11520–11523
Mwale SS, Hamusimbi C, Mwansa K (2003) Germination, emergence and growth of sunflower (Helianthus annuus L.) in response to osmotic seed priming. Seed Sci Technol 31(1):199–206
Nabanyumya R, Obua J, Tumwebaze SB (2015) Germination of Afrocarpus usambarensis and Podocarpus milanjianus seeds in Sango Bay, Uganda. Uganda J Agric Sci 16(2):231–244
Nancy W, Don ME, James B, Charles S (1997) Biological seed treatments: factors involved in efficacy. Hortic Sci 32:179–183
Nascimento WM, Cantliffe DJ, Huber DJ (2004) Ethylene evolution and endo-beta-mannanase activity during lettuceseed germination at high temperature. Sci Agric 61(2):156–163
Nascimento AR, Mouchrek Filho JE, Mouchrek Filho VE, Martins AG, Marinho SC, Serra CLM, Alves LMC (2005) Avaliação da sensibilidade de antimicrobianos a cepas de enterobacteriaceae isoladas de amostras de alface (Lactuca sativa) comercializada na cidade de São Luís-MA. Bol Cent Pesq Processamento Aliment 23(2):265–272
Nleya T, Ball RA, Vandenberg A (2005) Germination of common bean under constant and alternating cool temperatures. Can J Plant Sci 85(3):577–585
Okoro, S.P.A., Awodola, A.M. and Itolo, G.O. (1986) The impact of selected tree species on the soil properties in a Sudan Savanna Forest. In: Proceedings of 16th Annual Conference of Forestry Association of Nigeria, pp 660–667
Onochie CFA (1990) The dying dorest, declining forest resources of Nigeria. In: Keynote address 4th annual conferences of the Botanical Society of Nigeria. University of Nigeria, Nsukka
Osburn RM, Schroth MN (1989) Effect of osmopriming sugar beet seed on germination rate and incidence of Pythium ultimum damping-off. Plant Dis 73(1):21–24
Owonubi JJ, Otegbeye GO (2004) Disappearing forests: a review of the challenges for conservation of genetic resources and environmental management. J Res Manag 1:1–11
Ozturk M, Gemici M, Ozdemir F, Keyikci N (1994) Tohumçimlenmesiolayındabitkiselhor monlarınveçimlenmestimülatörününtuzstresiniazaltmadakirolü. UlusalBiyolojiKongresi, Edirne, pp 44–48
Palacios MG, Smits GB, Noguera R (1991) Presencia e influencia de algunos hongos patogenos en cultivos de Zinnia elegans Jacq. en la region central de Venezuela. Agron Trop 41(5-6):237–244
Paparella S, Araujo SS, Rossi G, Wijayasinghe M, Carbonera D, Balestrazzi A (2015) Seed priming: state of the art and new perspectives. Plant Cell Rep 34(8):1281–1293
Papavizas GC (1973) Status of applied biological control of soil-borne plant pathogens. Soil Biol Biochem 5(6):709–720
Parera CA, Cantliffe DJ (1994) Pre-sowing seed priming. Hort Rev 16:109–141
Phartyal SS, Baskin JM, Baskin CC, Thapliyal RC (2005) Physical dormancy in seeds of Dodonaea viscosa from India. Seed Sci Res 15:59–61
Pill WG (1995) Low water potential and presowing germination treatments to improve seed quality. In: Seed quality: basic mechanisms and agricultural implications. The Haworth Press, Binghamton, pp 319–359
Pill WG, Gunter JA (2001) Emergence and shoot growth of cosmos and marigold from paclobutrazol-treated seed. J Environ Hortic 19(1):11–14
Pill WG, Crossan CK, Frett JJ, Smith WG (1994) Matric and osmotic priming of Echinacea purpurea (L.) Moench seeds. Sci Hortic 59(1):37–44
Pinyopusarerk K, Boland DJ (1990) Casuarina junghuhniana: a highly adaptable tropical casuarina. NFT Highlights 90(4):1–2
Rafiq S, Iqbal T, Hameed A, Ali RZ, Rafiq N (2006) Morphobiochemical analysis of salinity stress response of wheat. Pak J Bot 38(5):1759–1767
Rao MSL, Kulkarni S, Lingaraju S, Nadaf HL (2009) Biopriming of seeds: potential tool in the integrated management of alternaria blight of sunflower. Helia 32(50):107–114
Rawat JMS, Tomar YK, Rawat V (2010) Effect of stratification on seed germination and seedling performance of wild pomegranate. J Am Sci 6(5):97–99
Revathi R, Mohan V, Jha MN (2013) Integrated nutrient management on the growth enhancement of Dalbergia sissoo Roxb. seedlings. J Acad Ind Res 1(9):550–557
Richardson MJ (1990) An annotated list of seed-borne diseases. International Seed Testing Association, ZĦrich
Rowse HR (1991) Methods of priming seeds. UK, Patent No. 2192781
Runkle ES, Heins RD, Cameron AC, Carlson WH (1999) Photoperiod and cold treatment regulate flowering of Rudbeckia fulgida Goldsturm. Hortscience 34(1):55–58
Schmidt L (2000) Guide to handling of tropical and subtropical forest seed. Danida Forest Seed Centre, Denmark, p 511
Sedghi M, Nemati A, Esmaielpour B (2010) Effect of seed priming on germination and seedling growth of two medicinal plants under salinity. Emirates J Food Agric 22(2):130
Shakarami B, Dianati TG, Tabari M, Behtari B (2011) The effect of priming treatments on salinity tolerance of Festuca arundinacea Schreb and Festuca ovina L. seeds during germination and early growth. Iran J Rangelands For Plant Breed Genet Res 18(2):318–328
Shin JS, Raymer P, Kim W (2006) Environmental factors influencing germination in seeded seashore paspalum. Hortscience 41(5):1330–1331
Singh AK, Karki K (2003) Effect of grading and GA3 on germination and seedling growth attributes in balsam. Progress Hortic 35(2):158–160
Sivaramakrishnan S, Patel VZ, Soman P (1990) Heat shock proteins of sorghum (Sorghum bicolor (L.) Moench) and pearl millet (Pennisetum glaucum (L.) R. Br.) cultivars with differing heat tolerance at seedling establishment stage. J Exp Bot 41:249–254
Sivritepe HO (2000) The effects of osmotic conditioning treatments on salt tolerance of onion seeds. In: 3rd National symposium on vegetable production, Isparta, Turkey. pp 475–481
Sivritepe HO, Dourado AM (1995) The effect of priming treatments on the viability and accumulation of chromosomal damage in aged pea seeds. Ann Bot 75(2):165–171
Ssenku JE, Ntale M, Backeus I, Oryem-Origa H (2017) Phytoremediation potential of Leucaena leucocephala for heavy metal-polluted and heavy metal-degraded environments. In: Phytoremediation potential of bioenergy plants. Springer, Singapore, pp 189–209
Subedi KD, Ma BL (2005) Seed priming does not improve corn yield in a humid temperate environment. Agron J 97(1):211–218
Suleman MK, Bhatt NR, Jacob S, Thomas RR (2011) Germination studies in Ochradenus baccatus Delile, Peganum harmala L. and Gynandriris sisyrinchium Parl. Res J Seed Sci 4:58–63
Suma N, Srimathi P, Roopa VM (2014) Influence of biofertilizer pelleting on seed and seedling quality characteristics of Sesamum indicum. Int J Curr Microbiol App Sci 3(6):591–594
Szopinska D, Tylkowska K (2003) Effect of osmopriming on location of seed-borne fungi in lettuce (Lactuca sativa) seeds. Phytopathol Pol 29:69–80
Tanaka Y, Edwards DGW (1986) An improved and more versatile method for prechilling Abies procera Rehd. seeds. Seed Sci Technol 14:457–464
Tarquis AM, Bradford KJ (1992) Prehydration and priming treatments that advance germination also increase the rate of deterioration of lettuce seeds. J Exp Bot 43(3):307–317
Taylor AG, Klein DE, Whitlow TH (1988) SMP: solid matrix priming of seeds. Sci Hortic 37:1–11
Taylor AG, Allen PS, Bennet MA, Bradford KJ, Burris JS, Misra MK (1998) Seed enhancements. Seed Sci Res 8:245–256
Toledo VM (1977) Pollination of Some Rain Forest Plants by Non-Hovering Birds in Veracruz, Mexico. Biotropica 9(4):262
Toorop PE, Van AC, Hilhorst HWM (1998) Endosperm cap weakening and endo-beta-mannanase activity during priming of tomato (Lycopersicon esculentum cv. Moneymaker) seeds are initiated upon crossing a threshold water potential. Seed Sci Res 8:483–491
Treshow M (1970) Mineral toxicity. In: Environment and plant response. McGraw-Hill, New York, pp 222–236
Trevisan S, Francioso O, Quaggiotti S, Nardi S (2010) Humic substances biological activity at the plant-soil interface: from environmental aspects to molecular factors. Plant Signal Behav 5(6):635–643
Troup RS (1921) The silviculture of Indian trees, vol 3. Clarendon Press, Oxford, pp 913–923
Varier A, Vari AK, Dadlani M (2010) Subcellular basis of seed priming. Curr Sci 99(4):450–456
Verma AN, Tandon P (2010) Seed germination and seedling growth in Pinus kesiya royle ex gord. I. Influence of imbibition, substrate pH and moisture level. Proc Indian Nat Sci Acad 50(3):326–331
Vertucci CW (1989) The kinetics of seed imbibition: controlling factors and relevance to seedling vigor. Seed Moisture, pp 93–115
Wang BSP, Lin TP, Chang TT (1998) Control of fungal growth with sphagnum for cold. 13(2):101–108
Wang HY, Chen CL, Sung JM (2003) Both warm water soaking and matriconditioning treatments enhance anti-oxidation of bitter gourd seeds germinated at sub-optimal temperature. Seed Sci Technol 31(1):47–56
Warren JE, Bennett MA (1997) Seed hydration using the drum priming system. Hortic Sci 32:1220–1221
Wu WS, Yang YH (1992) Alternaria blight: a seed-transmitted disease of zinnia in Taiwan. Plant Pathol Bull 1:115–123
Young JA, Clements CD (2003) Seed germination of willow species from a desert riparian ecosystem. J Range Manag 56:496–500
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Sisodia, A., Padhi, M., Pal, A.K., Barman, K., Singh, A.K. (2018). Seed Priming on Germination, Growth and Flowering in Flowers and Ornamental Trees. In: Rakshit, A., Singh, H. (eds) Advances in Seed Priming . Springer, Singapore. https://doi.org/10.1007/978-981-13-0032-5_14
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