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Isolation and Characterisation of Mutant Cell Lines

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Plant Cell and Tissue Culture

Abstract

It is perhaps dangerous to try to pinpoint the nascence of efforts at selection or isolation of modified phenotypes in vitro. Rather, awareness of the genetic potential of tissue culture dawned as researchers became conscious of the nature and scope of these cultures from numerous fundamental studies throughout the 1950s and 1960s. Those early to appreciate these possibilities deserve credit and this reviewer intends no disrespect to them in placing the watershed in the development of this field in the early 1970s with the reports on amino acid analogue resistant cell lines (Widholm, 1972a, b) and the selection of a streptomycin resistant mutant (Maliga et al., 1973). The former is noteworthy in that a rational selection strategy was devised to obtain a defined phenotype, namely tryptophan accumulation. Not only was the strategy carried through successfully, but characterisation of the resistant lines confirmed the predicted basis of the change, namely, reduced feedback sensitivity of the enzyme anthranilate synthetase.

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Authors
  1. Philip J. Dix
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Editors and Affiliations

  1. Laboratory of Plant Cell and Molecular Biology, Department of Horticultural Sciences, University of Florida, 1143 Fifield Hall, 32611-0690, Gainesville, FL, USA

    Indra K. Vasil

  2. Plant Physiology Research Group, Department of Biological Sciences, The University of Calgary, 2500 University Drive NW, T2N 1N4, Calgary, Alberta, Canada

    Trevor A. Thorpe

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© 1994 Springer Science+Business Media Dordrecht

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Dix, P.J. (1994). Isolation and Characterisation of Mutant Cell Lines. In: Vasil, I.K., Thorpe, T.A. (eds) Plant Cell and Tissue Culture. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-2681-8_5

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  • DOI: https://doi.org/10.1007/978-94-017-2681-8_5

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