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Somatic embryogenesis in neem (Azadirachta indica)

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Somatic Embryogenesis in Woody Plants

Part of the book series: Forestry Sciences ((FOSC,volume 44-46))

Abstract

Azadirachta indica A. Juss is commonly known as Neem or “Margosa”, and also Indian Lilac (synonym Melia azadirachta L.; Melia indica A. Juss Brand or Melia parviftora Moon; family Meliaceae). It is a native to Burma and dry arid regions of the Indian subcontinent. During the last century, it was introduced into the arid zones of Africa and is now being widely planted in Australia, Fiji, Mauritius, Central and South America, The Caribbean, Puerto Rico, Haiti, Philippines and other countries.

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References

  • Ammirato, P.V., 1983. Embryogenesis. In: D.A. Evans, W.R. Sharp, P.V. Ammirato et al. (Eds.), Handbook of Plant Cell Cultures. Techniques for Propagation and Breeding, pp. 82–113. Macmillan Publishing Co., New York.

    Google Scholar 

  • Butterworth, J.H. and E.D. Morgan, 1968. Isolation of a substance that suppresses feeding in locusts. Chem. Commun. Vol. 1: 23–24.

    Google Scholar 

  • Chopra, I.C., K.C. Gupta and B.N. Nazir, 1952. Preliminary study of antibacterial substances from Melia azadirachta. Indian J. Med. Res. 40: 511–515.

    PubMed  CAS  Google Scholar 

  • CSIR, 1985. The Wealth of India-I: Raw Materials, pp. 504–511. Government of India, New Delhi.

    Google Scholar 

  • Drew, R.A., 1993. Clonal propagation of Neem by tissue culture. Paper presented at the World Neem Conf., February 24–28, Bangalore.

    Google Scholar 

  • Gamborg, O.L., R.A. Miller and K. Ojima, 1968. Nutrient requirements of suspension cultures of soybean root cells. Exp. Cell Res. 50: 151–158.

    Article  PubMed  CAS  Google Scholar 

  • Gopinath, G., 1983. A clinical study of the therapeutic value of nimbin (from Melia azadirachta) on tropical eosinophilia. M.D. Thesis, University of Kerala, Kerala.

    Google Scholar 

  • Gupta, D. and B.C. Joshi, 1983. Electron impact studies of some sulphur compounds. In: A. Frigeria (Ed.), Recent Developments in Mass Spectrometry in Biochemistry, Medicine and Environmental Research, Part 8, pp. 143–157. Elsevier Scientific, Amsterdam.

    Google Scholar 

  • Gupta, P.K., R. Timmis, J. Pullman et al., 1991. In: I.K. Vasil (Ed.), Cell Culture and Somatic Cell Genetics of Plants, Vol. 8., pp. 75–92. Academic Press, New York.

    Google Scholar 

  • Gurumurthi, K. and S. Stanley Jagadees, 1992. Micropropagation of Azadirachta indica A. Juss from buds of coppice shoots. In: Proceedings of Workshop on Vegetative Propagation and Biotechnologies for Tree Improvement, Tirupathi (AP), pp. 137–140.

    Google Scholar 

  • Jaiswal, V.S. and P. Narayan, 1983. Regeneration of plantlets from the somatic tissue of some Indian trees. J. Exp. Biol. 22: 138–139.

    Google Scholar 

  • Joarder, N., R. Islam and O.I Joarder, 1993. Micropropagation of Azadirachta indica through axillary bud culture. Paper presented at the World Neem Conf., February 24–28, Bangalore.

    Google Scholar 

  • Jones, P.S., S.V. Ley, E.D. Morgan and D. Santafianos, 1989. The chemistry of the neem tree. In: M. Jacobson (Ed.), Focus on Phytochemical Pesticides, Vol. 1, The Neem Tree, p. 19. CRC Press, Boca Raton.

    Google Scholar 

  • Joshi, M.S. and S.R. Thengane, 1993. Potential application of in vitro methods for propagation of Neem. Paper presented at the World Neem Conf., February 24–28, Bangalore.

    Google Scholar 

  • Kendurkar, S.V. and A.F. Mascarenhas, 1987. Micropropagation of forest trees through tissue culture. In: G.M. Reddy (Ed.), Plant Cell and Tissue Culture of Economically Important Plants, pp. 181–190. Hyderabad, India.

    Google Scholar 

  • Khare, A.K., M.C. Srivastava, M.K. Sharma and J.D. Tewari, 1984. Antifertility activity of neem oil in rabbits and rats. Probe 23: 90–93.

    Google Scholar 

  • Koul, Opender., B.I. Murray and C.M. Ketkar, 1990. Properties and uses of neem, Azadirachta indica. Can. J. Bot. 68: 1–11.

    Article  Google Scholar 

  • Kraus, W., 1983. Biologically active compounds from Meliaceae. In: Proceedings of the 2nd International Conference on the Chemistry and Biotechnology of Biologically Active Natural Products, Budapest, pp. 331–345.

    Google Scholar 

  • Kraus, W., M. Bokel, A. Klenk and H. Pohnl, 1985. The structure of azadirachtin and 22, 23-dihydro-23-β-methoxyazadirachtin. Tetrahedron Lett. 26: 6435–35.

    Article  CAS  Google Scholar 

  • Lal, R., M. Gandhi, A. Sandaranarayan , V.S. Mathur and P.L. Sharma, 1987. Antifertility effect of Azadirachta indica oil administered to female albino rats on selected days of pregnancy. Fitoterapia 58: 239–242.

    Google Scholar 

  • Linsmaier, E.M. and F. Skoog, 1965. Organic growth factor requirements of Tobacco tissue cultures. Physiol. Plant. 18:100–127.

    Article  CAS  Google Scholar 

  • Mitra, C.R., 1963. Neem. Indian Council Oil Seed Committee, Himayatnagar, Hyderabad, pp. 15–68.

    Google Scholar 

  • Mitra, C.R., 1970. Studies of certain plants (inclusion of neem) extracts and isolates having pesticidal properties -final report. PL 480 Project No. A-T-ENT.

    Google Scholar 

  • Mohan Ram, H.Y. and N.B. Nair, 1993. Botany. In: N.S. Randhawa and B.S. Parmar (Ed.), Neem -Research and Development, pp. 6–26. SPS Publications, New Delhi.

    Google Scholar 

  • Muralidharan, E.M. and A.F. Mascarenhas, 1989. In vitro morphogenesis in Azadirachta indica A. Juss and Eucalyptus citriodora Hook. In: A.K. Kukreja, A.K. Mathur, P.S. Ahuja and R.S. Thakur (Ed.), Tissue Culture and Biotechnology of Medicinal and Aromatic Plants, pp. 49–55. Paramount Publ., New Delhi.

    Google Scholar 

  • Murashige, T. and F. Skoog, 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473–497.

    Article  CAS  Google Scholar 

  • Narayan, P. and V.S. Jaiswal, 1985. Plantlet regeneration from leaflet callus of Azadirachta indica A. Juss. J. Tree Sci. 4: 65–68.

    Google Scholar 

  • Nirmala Kumari, A., N.M. Rangaswamy and S.R. Sree Rangaswamy, 1993. Tissue Culture Studies in Neem. Paper presented at the World Neem Conf., February 24–28, Bangalore.

    Google Scholar 

  • Parmar, B.S. and C.M. Ketkar, 1993. Commercialization. In: N.S. Randhawa and B.S. Parmar (Eds.), Neem -Research and Development, pp. 270–283. SPS Publications, New Delhi.

    Google Scholar 

  • Radwanski, S., 1969. Improvement of red acid sand by neem tree (Azadirachta indica) in Sokoto, North Western State of Nigeria. J. Appl. Ecol. 6: 507–511.

    Article  Google Scholar 

  • Radwanski, S., 1977a. Neem Tree. 1. Commercial potential characteristics and distribution. World Crops Livest. 29: 62–63, 65–66.

    Google Scholar 

  • Radwanski, S., 1977b. Neem Tree. 2. Uses and Potential uses. World Crops Livest. 29: 111–113.

    Google Scholar 

  • Rangaswamy, N.S., 1986. Somatic embryogenesis in angiosperm cell tissue and organ cultures. Proc. Indian Acad. Sci. Plant Sci. 96: 247–271.

    CAS  Google Scholar 

  • Rangaswamy, N.S. and Promila, 1972. Morphogenesis of the adult embryos of Azadirachta indica. Z. Pflanz. Physiol. Bd. 67s: 377–379.

    Google Scholar 

  • Sanyal, M., A. Das, M. Bannerjee and P.C. Datta, 1981. In vitro hormone induced chemical and histological differentiation in stem callus of neem, Azadirachta indica A. Juss. J. Exp. Biol. 19: 1067–1068.

    CAS  Google Scholar 

  • Sanyal (Sarkar), M. and P.C. Datta, 1986. Nutritional environment and redifferentiation from cultured tissues of Azadirachta indica. Acta Hort. 188: 99–103.

    Google Scholar 

  • Sanyal (Sarkar), M., A. Mukherjee and P.C. Datta, 1988. Glycine on in vitro biosynthesis of Nimbin and β-sitosterol in tissues of Azadirachta indica. Curr. Sci. 57: 40–41.

    CAS  Google Scholar 

  • Sanyal, M., S. Tikadar and P.C. Datta, 1983. Nimbin and β-sitosterol in cotyledon and cultured tissues of Azadirachta indica. Indian Drugs 20: 479–481.

    CAS  Google Scholar 

  • Schmutterer, H., K.R.S Ascher and H. Rembold, 1981. Natural pesticides from the neem tree (Azadirachta indica) In: Proceedings of the 1st Int. Neem Conf., Roltach-Egern, p. 297.

    Google Scholar 

  • Schmutterer, H. and K.R.S. Ascher, 1984. Natural pesticides from the neem tree and other tropical plants. In: Proceedings of the 2nd Int. Neem Conf., Rauischholzhaussen, p. 43.

    Google Scholar 

  • Schulz, F.A., 1984. Tissue culture of Azadirachta indica A Juss. In: Proceedings of the 2nd Int. Neem Conf., Rauischholzhaussen, p. 83.

    Google Scholar 

  • Shrikhande, Medha., S.R. Thengane and A.F. Mascarenhas, 1993. Somatic embryogenesis and plant regeneration in Azadirachta indica A. Juss. In Vitro Cell. Dev. Biol. 29P: 38–42.

    Google Scholar 

  • Siddiqui, S., B.S. Siddiqui S. Faizi and T. Mahmood, 1988. Tetracyclic triterpenoids and their derivatives from Azadirachta indica. J. Nat. Prod. (Llyodia) 51: 30–43.

    Article  CAS  Google Scholar 

  • Sinniah, D. and G. Bhaskaran, 1981. Margosa oil poisoning as a cause of Reye’s syndrome. Lancet 1: 487–489.

    Article  PubMed  CAS  Google Scholar 

  • Thiagarajan, N. and P.M. Murali, 1993. High frequency regeneration of neem in vitro from immature embryo explants. Paper presented at the World Neem Conf., February 24–28, Bangalore.

    Google Scholar 

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Authors
  1. Shubhada Thengane
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  2. Medha Joshi
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  3. A. F. Mascarenhas
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Editor information

Editors and Affiliations

  1. Department of Plant Production, University of Helsinki, Helsinki, Finland

    S. Mohan Jain

  2. Weyerhaeuser Inc., Tacoma, Washington, USA

    Pramod K. Gupta

  3. Department of Forest Science, Texas A & M University, College Station, Texas, USA

    Ronald J. Newton

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© 1995 Springer Science+Business Media Dordrecht

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Thengane, S., Joshi, M., Mascarenhas, A.F. (1995). Somatic embryogenesis in neem (Azadirachta indica). In: Jain, S.M., Gupta, P.K., Newton, R.J. (eds) Somatic Embryogenesis in Woody Plants. Forestry Sciences, vol 44-46. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-0491-3_20

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  • DOI: https://doi.org/10.1007/978-94-011-0491-3_20

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-94-010-4220-8

  • Online ISBN: 978-94-011-0491-3

  • eBook Packages: Springer Book Archive

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