Abstract
Bananas (Musa ssp.) are among the world’s most important crops. In terms of gross value of production, they are the fourth most important global food crop and have an important socioeconomic and ecological role. Somatic embryogenesis (SE) is a developmental process, in which somatic cells differentiate into embryos which eventually develop and regenerate into plants. SE is exploited to generate a large quantity of very high economic value, genetically identical and disease-free plantlets. In bananas, the use of shoot apexes of axillary buds to induce SE resulted an alternative for plant regeneration through embryogenic cell suspension (ECS). The protocol has been scaled up to commercial laboratories for tissue culture (biofactories) for production of planting materials. The genetic stability of regenerated plants and high yields obtained under field conditions demonstrate the feasibility of scaling up this biotechnological protocol and adapting it to commercial production of planting materials to mitigate a critical bottleneck in the value chain of this important crop.
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Acknowledgments
The authors are thankful to Food and Agriculture Organization of the United Nations and the International Atomic Energy Agency through their Joint FAO/IAEA Programme of Nuclear Techniques in Food and Agriculture, for the fund support (CUB 15503) and thank profusely Dr. Nicolas Roux, Dr. Brad Till, and Dr. Chikelu Mba for their valuable suggestions and help, to technicians Nery Montano and Damicela Reinaldo for their significant contribution to the described protocols on this paper. Special thanks to MSc Geisy Díaz for her contribution in the translation of the manuscript.
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López, J., Rayas, A., Medero, V., Santos, A., Basail, M., Beovides, Y. (2022). Somatic Embryogenesis in Banana (Musa spp.). In: Ramírez-Mosqueda, M.A. (eds) Somatic Embryogenesis. Methods in Molecular Biology, vol 2527. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2485-2_8
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DOI: https://doi.org/10.1007/978-1-0716-2485-2_8
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