Abstract
The mitochondrial DNA of primary hexaploid triticale with and without timopheevi cytoplasm and the corresponding wheat cross parents were characterized by hybridization experiments using six different mitochondrial gene probes and four different restriction enzymes for Southern blot analyses with the novel non-radioactive digoxigenin technique. Via somatic embryogenesis 623 triticale plants were regenerated. The mitochondrial genome of 159 plants was investigated. Somaclonal variation of the chondriome was found to a high percentage in regenerated plants with timopheevi, but only to a low percentage in individuals with maintainer cytoplasm. In sixteen cases rearrangements in the mitochondrial DNA were detected. Furthermore, obvious alterations in the stoichiometry of specific hybridization bands have occurred. Some of these stoichiometric differences depend on the triticale-type.
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© 1996 Kluwer Academic Publishers
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Weigel, R., Wolf, M., Stierle, M., Stoesser, T., Hesemann, CU. (1996). Somaclonal Variation Detected on the Molecular Level in the Mitochondrial Genome of Regenerated Primary CMS Triticale Plants. In: Guedes-Pinto, H., Darvey, N., Carnide, V.P. (eds) Triticale: Today and Tomorrow. Developments in Plant Breeding, vol 5. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-0329-6_45
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DOI: https://doi.org/10.1007/978-94-009-0329-6_45
Publisher Name: Springer, Dordrecht
Print ISBN: 978-94-010-6634-1
Online ISBN: 978-94-009-0329-6
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