Abstract
Acetic acid bacteria are currently accommodated in the acetous group, the family Acetobacteraceae, the class Alphaproteobacteria, based on phylogeny, physiology, and ecology. The acetic acid bacteria are classified at present in 17 genera, of which many species have been reported in the genera Acetobacter, Gluconobacter, Gluconacetobacter, Asaia, and Komagataeibacter. Of the remaining 12 genera, Acidomonas, Kozakia, Swaminathania, Saccharibacter, Neoasaia, Granulibacter, Tanticharoenia, Ameyamaea, Endobacter, Nguyenibacter, and Swingsia are monotypic; the genus Neokomagataea contains two species. In the class Gammaproteobacteria, the genus Frateuria has been mentioned taxonomically as pseudacetic acid bacteria. In addition, isolation and identification of acetic acid bacteria are described.
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Keywords
- Acetic acid bacteria
- Acetobacteraceae
- Alphaproteobacteria
- The acetous group
- Acetobacter
- Acetobacter aceti
- Gluconobacter
- Gluconobacter oxydans
- Pseudacetic acid bacteria
- Gammaproteobacteria
- Frateuria
1.1 Introduction
The generic name Acetobacter , the oldest name for acetic acid bacteria, was introduced by Beijerinck (1898). However, there is no record of the formal proposal of the generic name as a genus (Komagata et al. 2014; Buchanan et al. 1966; Kluyver 1983). Skerman et al. (1980) cited, ‘as it occurs today’ in the Approved Lists of Bacterial Names 1980, the generic name Acetobacter as Acetobacter Beijerinck 1898, in which the type species was designated as Acetobacter aceti (Pasteur 1864) Beijerinck 1898.
Asai (1935) divided the acetic acid bacteria into two genera: one genus included the species that oxidized ethanol more intensely than d-glucose and had the capability of oxidizing acetic acid to carbon dioxide and water, and the other contained the species that are especially isolated from fruit, oxidized d-glucose more intensely than ethanol, and had no capability of oxidizing acetic acid. For the latter genus, the name Gluconobacter Asai 1935 was proposed.
Almost 20 years later, the genus ‘Acetomonas’ Leifson 1954 was introduced for species that had polar flagellation and were non acetate oxidizing (Leifson 1954). In contrast, the strains of the genus Acetobacter had peritrichous flagellation and the capability of oxidizing acetic acid to carbon dioxide and water. The proposals of the two generic names were, of course, the result of confusion in the systematics of acetic acid bacteria (Shimwell 1958; Asai and Shoda 1958; Shimwell and Carr 1959).
De Ley (1961) recognized the priority of the generic name Gluconobacter over the generic name ‘Acetomonas.’ Gluconobacter oxydans (Henneberg 1897) De Ley 1961 was designated as the type species of the genus Gluconobacter, because Asai (1935) did not designate the type species (De Ley 1961; De Ley and Frateur 1970).
In acetic acid bacteria, Asai et al. (1964) reported two types of intermediate strains in addition to strains of the genera Acetobacter and Gluconobacter. One type of the strains had peritrichous flagellation, and the other had polar flagellation despite being acetate oxidizing. The genera Acetobacter and Gluconobacter were distinguished chemotaxonomically from each other by the presence of the major ubiquinone homologues, that is, Q-9 for the former and Q-10 for the latter (Yamada et al. 1969a). The peritrichously flagellated intermediate strains, which were formerly classified as ‘ Gluconobacter liquefaciens ’ (Asai 1935; Asai and Shoda 1958; Asai 1968) and later regarded as pigment-producing strains of Acetobacter aceti (Carr and Shimwell 1960; Kimmit and Williams 1963), had Q-10, which was quite different from the type strain of Acetobacter acet i (Q-9), the type species of the genus Acetobacter , but similar to strains of the genus Gluconobacter . On the contrary, the polarly flagellated intermediate strains, which were once classified as ‘Acetobacter aurantium’ (sic) (Kondo and Ameyama 1958), had Q-8, which was never found in any other strains of acetic acid bacteria, and these strains were later classified as Frateuria aurantia (ex Kondo and Ameyama 1958) Swings et al. 1980 (Swings et al. 1980).
In the Approved Lists of Bacterial Names 1980, the Q-10-equipped peritrichously flagellated intermediate strains were listed as Acetobacter aceti subsp. liquefaciens (Asai 1935) De Ley and Frateur 1974 (Skerman et al. 1980). The Q-10-equipped strains, which were classified as Acetobacter liquefaciens (Asai 1935) Gosselé et al. 1983 (= A. aceti subsp. liquefaciens) and as Acetobacter xylinus (Brown 1886) Yamada 1984 [= A. aceti subsp. xylinus corrig. (Brown 1886) De Ley and Frateur 1974], were distinguished from the Q-9-equipped strains within the genus Acetobacter at the subgeneric level, and the subgenus Gluconacetobacter corrig. Yamada and Kondo 1984 was proposed (Yamada and Kondo 1984). However, the subgenus was not accepted in the classification of acetic acid bacteria, along with the genus Acidomonas Urakami et al. 1989 for the methanol-assimilating acetic acid bacterium, Acetobacter methanolicus Uhlig et al. 1986 (Swings 1992; Sievers et al. 1994).
The subgenus Gluconacetobacter was phylogenetically discussed on the basis of the partial 16S rRNA sequences, along with the genus Acidomonas , and elevated at the generic level as the genus Gluconacetobacter Yamada et al. 1998 with a concomitant existence of the genus Acidomonas (Yamada et al. 1997). The type species was designated as Gluconacetobacter liquefaciens (Asai 1935) Yamada et al. 1998.
In the genus Gluconacetobacter, there were two subclusters in the phylogenetic trees based on 16S rRNA gene sequences (Franke et al. 1999; Yamada et al. 2000). Later, the existence of two phylogenetic groups, that is, the Gluconacetobacter liquefaciens group and the Gluconacetobacter xylinus group, was suggested to be distinguished at the generic level on the basis of morphological, physiological, chemotaxonomic, and ecological characteristics (Yamada and Yukphan 2008). For the latter group, the genus Komagataeibacter Yamada et al. 2013 was introduced with the type species, Komagataeibacter xylinus (Brown 1886) Yamada et al. 2013 (Yamada et al. 2012a, b).
At the present time, 17 genera are recognized in acetic acid bacteria or the acetous group of the family Acetobacteraceae Gillis and De Ley 1980, the class Alphaproteobacteria Stackebrandt et al. 1988, viz., Acetobacter Beijerinck 1898, Gluconobacter Asai 1935, Acidomonas Urakami et al. 1989 emend. Yamashita et al. 2004, Gluconacetobacter Yamada et al. 1998, Asaia Yamada et al. 2000, Kozakia Lisdiyanti et al. 2002, Swaminathania Loganathan and Nair 2004, Saccharibacter Jojima et al. 2004, Neoasaia Yukphan et al. 2006, Granulibacter Greenberg et al. 2006, Tanticharoenia Yukphan et al. 2008, Ameyamaea Yukphan et al. 2010, Neokomagataea Yukphan et al. 2011, Komagataeibacter Yamada et al. 2013, Endobacter Ramírez-Bahena et al. 2013, Nguyenibacter Vu et al. 2013, and Swingsia Malimas et al. 2014 (Fig. 1.1). Of the 17 genera, the 5 genera Acetobacter , Gluconobacter , Gluconacetobacter , Asaia , and Komagataeibacter each include a large number of species. However, the remaining 12 genera are monotypic, that is, contain only 1 species, except for the genus Neokomagataea , which consists of 2 species.
1.2 Isolation of Acetic Acid Bacteria
The isolation of acetic acid bacteria is in general carried out by an enrichment culture approach (Komagata et al. 2014; Sievers and Swings 2005a). A medium for the enrichment procedure and the isolation of acetic acid bacteria, designated as the pH 3.5 medium (Yamada et al. 1999), is composed, for example, of 1.0 % d-glucose (w/v), 0.5 % ethanol (99.8 %) (v/v), 0.3 % peptone (w/v), 0.2 % yeast extract (w/v), and 0.01 % cycloheximide (w/v), and adjusted at pH 3.5 with hydrochloric acid. In the isolation of acetic acid bacteria capable of fixing atmospheric nitrogen, the LGI medium that contains 10.0 % sucrose (w/v), 0.06 % KH2PO4 (w/v), 0.02 % K2HPO4 (w/v), 0.02 % MgSO4 (w/v), 0.002 % CaCl2 (w/v), 0.001 % FeCl3 (w/v), and 0.0002 % Na2MoO4 (w/v) is used at pH 6.0 (Cavalcante and Döbereiner 1988). When microbial growth is seen in the LGI medium, the culture is transferred to the pH 3.5 medium mentioned previously (Vu et al. 2013). To obtain and purify candidates of acetic acid bacteria, the culture in the pH 3.5 medium is streaked onto agar plates, which are composed of 2.0 % d-glucose (w/v), 0.5 % ethanol (99.8 %) (v/v), 0.3 % peptone (w/v), 0.3 % yeast extract (w/v), 0.7 % calcium carbonate (e.g., precipitated by Japanese Pharmacopoeia) (w/v), and 1.5 % agar (w/v) (Yamada et al. 1999), and the resulting colonies that dissolve calcium carbonate on the agar plates are picked up, inoculated, and incubated on agar slants with the same composition as the agar plates for temporary preservation. The strains isolated were examined again for growth on the pH 3.5 medium.
When the composition, especially the carbon sources, of the medium in the enrichment procedure is changed, the selective isolation of acetic acid bacteria can be expected. In fact, strains of Asaia bogorensis and Asaia siamensis were first isolated by the use of d-sorbitol or dulcitol instead of d-glucose (Yamada et al. 2000; Katsura et al. 2001). Several kinds of media employed for the enrichment procedure result in the effective isolation of acetic acid bacteria (Lisdiyanti et al. 2003b; Suzuki et al. 2010). Instead of the pH 3.5 medium, the pH 4.5 medium containing 0.03 % acetic acid (v/v) can be used (Yamada et al. 1976).
In the genera that are not monotypic, including more than several species and therefore restricted to Acetobacter , Gluconobacter , Gluconacetobacter , Asaia , and Komagataeibacter (which are supposed to be taxonomically and ecologically in common but not in rare existence), the generic-level, routine identification for certain strains of acetic acid bacteria can be done by the combination of only two conventional phenotypic tests composed of acetate and lactate oxidation and the production of acetic acid from ethanol (Yamada and Yukphan 2008).
In strains to be assigned to the genus Acetobacter , a deep blue color appears quickly and clearly in the acetate and lactate oxidation tests, and acetic acid is produced in the acetic acid production test (Asai et al. 1964; Yamada and Yukphan 2008). In acetate and lactate oxidation, strains to be assigned to the genus Gluconobacter show a clear yellow color, and the color change to blue is not so vigorous in strains to be assigned to the genera Gluconacetobacter and Komagataeibacter , in contrast to the genus Acetobacter . The latter two genera, Gluconacetobacter and Komagataeibacter , are additionally discriminated from each other by water-soluble brown pigment production and cell motility. Strains to be assigned to the former generally produce a water-soluble brown pigment, being motile, but strains to be assigned to the latter do not, being non motile. Strains to be assigned to the genus Asaia show no or little acetic acid production from ethanol, differing from the aforementioned four genera, and the color change is very slow in acetate and lactate oxidation. The two conventional tests just described are useful, especially when a large number of isolates are routinely identified or classified at the generic level.
To isolate acetic acid bacteria, sugary and alcoholic materials have widely been utilized as isolation sources. In such cases, the habitats of the acetic acid bacteria are to be the isolation sources (Komagata et al. 2014; Kersters et al. 2006; Sievers and Swings 2005a). Recently, acetic acid bacteria have been found ecologically in a wide variety of isolation sources, such as activated sludges, rhizosphere soils, soils, pollen, human patients, mosquitoes, a stone chamber of a tumulus, and nodules (Komagata et al. 2014; Kersters et al. 2006; Sievers and Swings 2005a). In addition, acetic acid bacteria that grow on nitrogen-free media have been found (Gillis et al. 1989; Fuentes-Ramírez et al. 2001; Samaddar et al. 2011; Vu et al. 2013).
Most acetic acid bacteria can be maintained at 4 °C for 1 month on agar slants containing an appropriate medium. Long-term preservation of acetic acid bacteria can be achieved by lyophilization or by storage in liquid nitrogen, or by cryoconservation at −80 °C by the use of low-temperature refrigerators and appropriate cryoprotectants (Komagata et al. 2014; Kersters et al. 2006; Sievers and Swings 2005a).
1.3 Identification of Acetic Acid Bacteria
When a certain strain of acetic acid bacteria is isolated, the strain will be assigned to a proper or suitable systematic or taxonomic position. Such a process is called identification. The identification consists of two levels, genus level and species level.
To select acetic acid bacteria from a number of the strains isolated, it is suitable to test the strains for growth on a pH 3.5 medium, which contains, for example, 1.0 % d-glucose (w/v), 0.5 % ethanol (99.8 %) (v/v), 0.3 % peptone (w/v), and 0.2 % yeast extract (w/v); the pH is adjusted to 3.5 with hydrochloric acid (Yamada et al. 1999). A pH 4.0 medium can be used for the growth test. If a certain strain is an acetic acid bacterium, appropriate growth can be seen. If the pH of the medium is adjusted to 4.5, bacteria other than acetic acid bacteria sometimes can grow.
For generic-level identification, the candidates of the acetic acid bacteria obtained are in general subjected to 16S rRNA gene sequence analysis, especially to the construction of phylogenetic trees based on 16S rRNA gene sequences (Komagata et al. 2014). When the phylogenetic trees are constructed by the three methods, viz., the neighbor-joining, maximum parsimony, and maximum likelihood methods, the candidates may be assignable to new taxa, such as new genera (Yamada and Yukphan 2008). On the other hand, some phenotypic feature analyses are applicable to the routine identification of the candidates (Table 1.1).
For specific-level identification, whole-genome DNA–DNA hybridization is necessary and inevitable for the precise identification of the strains that have already been identified or classified at the generic level (Komagata et al. 2014). Of the phenotypic features used for the specific-level identification, acid production from different carbon sources and growth on different carbon sources are generally utilized; however, precise identification would hardly be expected.
Recently, many taxonomic methods have been reported (Komagata et al. 2014; Sievers and Swings 2005a; Cleenwerck and De Vos 2008), for example, isoprenoid quinone analysis and fatty acid composition analysis as chemotaxonomic methods and DNA base composition determination, and 16S–23S rRNA gene internally transcribed spacer (ITS) sequencing and restriction analysis of ITS as DNA-based molecular methods, in addition to the phenotypic feature analysis, 16S rRNA gene sequence analysis, and the whole-genome DNA–DNA hybridization . The combination of these methods gives more precise information for the identification and the classification of acetic acid bacteria.
1.4 Genera and Species in Acetic Acid Bacteria
The acetic acid bacteria classified in the acetous group constitute the family Acetobacteraceae Gillis and De Ley 1980, the class Alphaproteobacteria Stackebrandt et al. 1988, together with the acidophilic group (Komagata et al. 2014; Sievers and Swings 2005a; Gillis and De Ley 1980; Stackebrandt et al. 1988). The type genus of the family is Acetobacter . Seventeen genera are reported (Table 1.1). The genera and the species listed below are ordered chronologically, because they have their own respective long (or not so long) histories in transitions of generic and specific circumscriptions and in selection of isolation sources.
1.4.1 Acetobacter Beijerinck 1898
A.ce.to.bac’ter. L. neut. n. acetum, vinegar; N. L. masc. n. bacter, rod; N. L. masc. n. Acetobacter, vinegar rod.
The genus Acetobacter is the oldest in the classification of acetic acid bacteria and the type genus of the family Acetobacteraceae . In the Approved Lists of Bacterial Names 1980, the three species Acetobacter aceti , Acetobacter pasteurianus, and Acetobacter peroxydans were listed, with their nine subspecies (Skerman et al. 1980). The genus is related phylogenetically to the genera Gluconobacter , Neokomagataea , Swingsia , and Saccharibacter . In the genus Acetobacter, there are two phylogenetically different groups: the Acetobacter aceti group and the Acetobacter pasteurianus group.
Cells are gram negative, ellipsoidal to rod shaped, measuring 0.4–1.0 by 1.2–3.0 μm, rarely longer. Cells occur singly or in short chains and occasionally long chains. Peritrichously flagellated when motile; however, Acetobacter nitrogenifigens exceptionally has polar flagella (Dutta and Gachhui 2006). Colonies are generally circular, smooth, entire, convex, cream color to beige, opaque, and butyrous on glucose/ethanol/yeast extract/peptone agar.
Strictly aerobic. Catalase positive, but negative in Acetobacter peroxydans. Oxidase negative. Acetic acid is produced from ethanol. Acetate and lactate are oxidized to carbon dioxide and water. Does not grow on glutamate agar and very weakly on mannitol agar. Dihydroxyacetone is not usually produced from glycerol, but is produced by a few species. d-Gluconate is produced from d-glucose by all the species, 2-keto-d-gluconate by a considerable number of species, and 5-keto-d-gluconate by a few species. 2,5-Diketo-d-gluconate is not generally produced. Acid production depends on the kind of sugars, sugar alcohols, and alcohols as well as on the kinds of species and strains. In the type strain of Acetobacter aceti , acid is produced from l-arabinose, d-xylose, d-glucose, d-galactose, d-mannose, or ethanol (Lisdiyanti et al. 2000). Ammoniac nitrogen is in general hardly utilized.
The optimal growth temperature is around 30 °C. Most species are able to grow at 37 °C but not at 45 °C. Grows at pH 3.5. Most species are not able to grow on 30 % d-glucose (w/v). The major cellular fatty acid is C18:1ω7c. The major quinone is Q-9. The DNA G+C content is 53.5–60.7 mol%. For more details of the characteristics, see Komagata et al. (2014).
The type species of the genus is Acetobacter aceti (Pasteur 1864) Beijerinck 1898. Twenty-five species are reported.
1.4.1.1 Acetobacter aceti (Pasteur 1864) Beijerinck 1898
For the characteristics of the species, refer to Lisdiyanti et al. (2000), Gosselé et al. (1983b), Komagata et al. (2014), and Sievers and Swings (2005b).
The type strain is ATCC 15973T (= DSM 3508T = JCM 7641T = LMG 1261T = LMG 1504T = NBRC 14818T = NCIMB 8621T), isolated from beechwood shavings of a vinegar plant. The DNA G+C content of the type strain is 57.2 mol%.
1.4.1.2 Acetobacter pasteurianus (Hansen 1879) Beijerinck and Folpmers 1916
For the characteristics of the species, refer to Beijerinck and Folpmers (1916), Lisdiyanti et al. (2000), Gosselé et al. (1983b), Komagata et al. (2014), and Sievers and Swings (2005b).
The type strain is LMG 1262T (=ATCC 33445T = DSM 3509T = JCM 7640T = LMD 22.1T), isolated from beer, Netherlands. The DNA G+C content of the type strain is 52.7 mol%.
1.4.1.3 Acetobacter peroxydans Visser’t Hooft 1925
For the characteristics of the species, refer to Visser’t Hooft (1925), Lisdiyanti et al. (2000), Gosselé et al. (1983b), Komagata et al. (2014), and Sievers and Swings (2005b).
The type strain is NBRC 13755T (=ATCC 12874T = JCM 25077T = LMG 1635T), isolated from ditch water, Delft, Netherlands. The DNA G+C content of the type strain is 60.3 mol%.
1.4.1.4 Acetobacter pomorum Sokollek, Hertel and Hammes 1998
For the characteristics of the species, refer to Sokollek et al. (1998).
The type strain is LTH 2458T (= CIP 105762T = DSM 11825T = LMG 18848T), isolated from a submerged cider vinegar fermentation at a factory in the southern part of Germany. The DNA G+C content of the type strain is 50.5 mol%.
1.4.1.5 Acetobacter estunensis (Carr 1958) Lisdiyanti, Kawasaki, Seki, Yamada, Uchimura and Komagata 2001
Basonym: Acetobacter pasteurianus subsp. estunensis (Carr 1958) De Ley and Frateur 1974.
For the characteristics of the species, refer to Lisdiyanti et al. (2000).
The type strain is NBRC 13751T (= ATCC 23753T = DSM 4493T = JCM 21172T = LMG 1626T = NCIMB 8935T), isolated from cider, Bristol. The DNA G+C content of the type strain is 59.7 mol%.
1.4.1.6 Acetobacter lovaniensis (Frateur 1950) Lisdiyanti, Kawasaki, Seki, Yamada, Uchimura and Komagata 2001
Basonym: Acetobacter pasteurianus subsp. lovaniensis (Frateur 1950) De Ley and Frateur 1974.
For the characteristics of the species, refer to Lisdiyanti et al. (2000).
The type strain is NBRC 13753T (=ATCC 12875T= DSM 4491T = JCM 17121T = LMG 1579T = LMG 1617T = NCIMB 8620T), isolated from sewage on soil by J. Frateur in 1929. The DNA G+C content of the type strain is 58.6 mol%.
1.4.1.7 Acetobacter orleanensis (Henneberg 1906) Lisdiyanti, Kawasaki, Seki, Yamada, Uchimura and Komagata 2001
Basonym: Acetobacter aceti subsp. orleanensis (Henneberg 1906) De Ley and Frateur 1974.
For the characteristics of the species, refer to Lisdiyanti et al. (2000).
The type strain is NBRC 13752T (= ATCC 12876T = DSM 4492T = JCM 7639T = LMG 1583T = NCIMB 8622T), isolated from beer by J. Frateur in 1929. The DNA G+C content of the type strain is 58.6 mol%.
1.4.1.8 Acetobacter indonesiensis Lisdiyanti, Kawasaki, Seki, Yamada, Uchimura and Komagata 2001
For the characteristics of the species, refer to Lisdiyanti et al. (2000).
The type strain is 5H-1T (= JCM 10948T = LMG 19824T = NBRC 16471T = NRIC 0313T), isolated from fruit of zirzak (Annona muricata) in Indonesia. The DNA G+C content of the type strain is 53.7 mol%.
1.4.1.9 Acetobacter tropicalis Lisdiyanti, Kawasaki, Seki, Yamada, Uchimura and Komagata 2001
For the characteristics of the species, refer to Lisdiyanti et al. (2000).
The type strain is Ni-6bT (= JCM 10947T = LMG 19825T = NBRC 16470T = NRIC 0312T), isolated from coconut (Coccos nucifera) in Indonesia. The DNA G+C content of the type strain is 55.9 mol%.
1.4.1.10 Acetobacter cerevisiae Cleenwerck, Vandemeulebroecke, Janssens and Swings 2002
For the characteristics of the species, refer to Cleenwerck et al. (2002).
The type strain is LMG 1625T (= ATCC 23765T = DSM 14362T = JCM 17273T = NCIMB 8894T), isolated from beer (ale) in storage at Toronto, Canada. The DNA G+C content of the type strain is 57.6 mol%.
1.4.1.11 Acetobacter malorum Cleenwerck, Vandemeulebroecke, Janssens and Swings 2002
For the characteristics of the species, refer to Cleenwerck et al. (2002).
The type strain is LMG 1746T (= DSM 14337T = JCM 17274T), isolated from a rotten apple in Ghent, Belgium. The DNA G+C content of the type strain is 57.2 mol%.
1.4.1.12 Acetobacter cibinongensis Lisdiyanti, Kawasaki, Seki, Yamada, Uchimura and Komagata 2002
For the characteristics of the species, refer to Lisdiyanti et al. (2001).
The type strain is 4H-1T (= CIP 107380T = DSM 15549T = JCM 11196T = NBRC 16605T), isolated from mountain soursop (Annona montana) in Indonesia. The DNA G+C content of the type strain is 54.5 mol%.
1.4.1.13 Acetobacter orientalis Lisdiyanti, Kawasaki, Seki, Yamada, Uchimura and Komagata 2002
For the characteristics of the species, refer to Lisdiyanti et al. (2001).
The type strain is 21F-2T (= CIP 107379T = DSM 15550T = JCM 11195T = NBRC 16606T = NRIC 0481T), isolated from canna flower (Canna hybrida) in Indonesia. The DNA G+C content of the type strain is 52.3 mol%.
1.4.1.14 Acetobacter syzygii Lisdiyanti, Kawasaki, Seki, Yamada, Uchimura and Komagata 2002
For the characteristics of the species, refer to Lisdiyanti et al. (2001).
The type strain is 9H-2T (= CIP 107378T = DSM 15548T = JCM 11197T = NBRC 16604T = NRIC 0483T), isolated from fruit of Malay rose apple (Syzygium malaccense) in Indonesia. The DNA G+C content of the type strain is 55.3 mol%.
1.4.1.15 Acetobacter nitrogenifigens Dutta and Gachhui 2006
For the characteristics of the species, refer to Dutta and Gachhui (2006).
The type strain is RG1T (= LMG 23498T = MTCC 6912T), isolated from Kombucha tea. The DNA G+C content of the type strain is 64.1 mol%.
1.4.1.16 Acetobacter oeni Silva, Cleenwerck, Rivas, Swings, Trujillo, Willems and Velázquez 2006
For the characteristics of the species, refer to Silva et al. (2006).
The type strain is B13T (= CECT 5830T = LMG 21952T), isolated from spoiled red wine of the Dão region, Portugal. The DNA G+C content of the type strain is 58.1 mol%.
1.4.1.17 Acetobacter ghanensis Cleenwerck, Camu, Engelbeen, De Winter, Vandemeulebroecke, De Vos and De Vuyst 2007
For the characteristics of the species, refer to Cleenwerck et al. (2007).
The type strain is R-29337T (= 430AT = DSM 18895T = LMG 23848T), isolated from a traditional heap fermentation of Ghanaian cocoa beans. The DNA G+C content of the type strain is 57.3 mol%.
1.4.1.18 Acetobacter senegalensis Ndoye, Cleenwerck, Engelbeen, Dubois-Dauphin, Guiro, Van Trappen, Willems and Thonart 2007
For the characteristics of the species, refer to Ndoye et al. (2007).
The type strain is CWBI-B418T (= DSM 18889T = LMG 23690T), isolated from mango fruit in Senegal (Sub-Saharan Africa). The DNA G+C content of the type strain is 56.0 mol%.
1.4.1.19 Acetobacter fabarum Cleenwerck, González, Camu, Engelbeen, De Vos and De Vuyst 2008
For the characteristics of the species, refer to Cleenwerck et al. (2008).
The type strain is 985T (= R-36330T = DSM 19596T = LMG 24244T), isolated from Ghanaian cocoa heap fermentation. The DNA G+C content of the type strain is 57.6 mol%.
1.4.1.20 Acetobacter farinalis Tanasupawat, Kommanee, Yukphan, Muramatsu, Nakagawa and Yamada 2011
For the characteristics of the species, refer to Tanasupawat et al. (2011a).
The type strain is G360-1T (= BCC 44845T = NBRC 107750T = PCU 319T), isolated from fermented rice flour. The DNA G+C content of the type strain is 56.3 mol%.
1.4.1.21 Acetobacter papayae Iino, Suzuki, Kosako, Ohkuma, Komagata and Uchimura 2013
For the characteristics of the species, refer to Iino et al. (2012a).
The type strain is 1-25T (= JCM 25143T = LMG 26456T = NRIC 0655T), isolated from a papaya fruit, Okinawa, Japan. The DNA G+C content of the type strain is 60.5 mol%.
1.4.1.22 Acetobacter okinawensis Iino, Suzuki, Kosako, Ohkuma, Komagata and Uchimura 2013
For the characteristics of the species, refer to Iino et al. (2012a).
The type strain is 1-35T (= JCM 25146T = LMG 26457T = NRIC 0658T), isolated from a piece of a stem of sugarcane, Okinawa, Japan. The DNA G+C content of the type strain is 59.3 mol%.
1.4.1.23 Acetobacter persici corrig. Iino, Suzuki, Kosako, Ohkuma, Komagata and Uchimura 2013
For the characteristics of the species, refer to Iino et al. (2012a).
The type strain is T-120T (= JCM 25330T = LMG 26458T), isolated from a peach fruit, Okinawa, Japan. The DNA G+C content of the type strain is 58.7 mol%.
1.4.1.24 Acetobacter lambici Spitaels, Li, Wieme, Balzarini, Cleenwerck, Van Landschoot, De Vuyst and Vandamme 2014
For the characteristics of the species, refer to Spitaels et al. (2014a).
The type strain is LMG 27439T (= DSM 27328T), isolated from fermenting lambic beer. The DNA G+C content of the type strain is 56.2 mol%.
1.4.1.25 Acetobacter sicerae Li, Wieme, Spitaels, Balzarini, Nunes, Manaia, Van Landschoot, De Vuyst, Cleenwerck and Vandamme 2014
For the characteristics of the species, refer to Li et al. (2014).
The type strain is LMG 1531T (= NCIMB 8941T), isolated from traditionally produced kefir. The DNA G+C content of the type strain is 58.3 mol%.
1.4.2 Gluconobacter Asai 1935
Glu.co.no.bac’ter. N. L. neut. n. acidum gluconicum, gluconic acid; N. L. masc. n. bacter, rod; N. L. masc. n. Gluconobacter, gluconate rod.
The genus Gluconobacter was proposed by Asai (1935), who selected a variety of fruits for isolation of acetic acid bacteria and found two taxonomic groups in the isolated strains on the oxidation of ethanol and d-glucose. One had intense ethanol oxidizability rather than d-glucose and oxidized acetic acid to carbon dioxide and water, and the other had intense glucose oxidizability rather than ethanol and did not oxidize acetic acid. For the latter group, the generic name Gluconobacter was given. In the Approved Lists of Bacterial Names 1980, the only species, Gluconobacter oxydans , was listed with its five subspecies (Skerman et al. 1980). The DNA G+C content of the species was 54.2–62.8 mol%, with the range of 8.6 mol% (Yamada et al. 1981b).
Cells are gram negative, ellipsoidal to rod shaped, measuring 0.4–1.2 by 1.0–3.0 μm, and polarly flagellated when motile. Colonies are smooth, raised to convex, entire and glistening on ethanol/glucose/yeast extract/calcium carbonate/agar. Some strains produce pink colonies.
Strictly aerobic. Catalase positive and oxidase negative. Acetic acid is produced from ethanol. Acetate and lactate are not oxidized. Grows on mannitol agar, but not on glutamate agar. Dihydroxyacetone is produced from glycerol. d-Gluconate, 2-keto-d-gluconate, and 5-keto-d-gluconate are produced from d-glucose, and a few strains produce 2,5-diketo-d-gluconate. A water-soluble brown pigment is produced in strains of a few species. Acid is produced from l-arabinose, d-xylose, d-glucose, d-galactose, d-mannose, d-fructose, melibiose, d-mannitol, d-sorbitol, glycerol, and ethanol. Grows on d-glucose, d-fructose, d-mannitol, d-sorbitol, and glycerol. Strains of several species require nicotinic acid for growth.
Optimum temperature for growth is 25 °–30 °C. Many species grow at 35 °C, and a few species grow at 37 °C. Optimum pH for growth is around pH 5.5. Most species grow at pH 3.5. acid is C18:1ω7c. The major ubiquinone is Q-10. The DNA G+C content is 54.0–61.5 mol%. Strains of Gluconobacter are isolated from fruits, flowers, and other sugar-rich materials. For more details of characteristics, see Komagata et al. (2014).
The type species of the genus is Gluconobacter oxydans (Henneberg 1897) De Ley 1961. Fourteen species are reported.
1.4.2.1 Gluconobacter oxydans (Henneberg 1897) De Ley 1961
For the characteristics of the species, refer to Asai et al. (1964), Yamada et al. (1981a, b), Gosselé et al. (1983a), Yamada and Akita (1984), Tanaka et al. (1999), Katsura et al. (2002), Komagata et al. (2014), and Sievers and Swings (2005d).
The type strain is ATCC 19357T (= DSM 3503T = DSM 7145T = JCM 7642T = LMG 1408T = NBRC 14819T = NCIMB 9013T), isolated from beer by J.G. Carr. The DNA G+C content of the type strain is 60.3 mol%.
1.4.2.2 Gluconobacter cerinus (ex Asai 1935) Yamada and Akita 1984 emend. Katsura, Yamada, Uchimura and Komagata 2002
Synonym: Gluconobacter asaii Mason and Claus 1989.
For the characteristics of the species, refer to Yamada and Akita (1984), Yamada et al. (1984), Mason and Claus (1989), and Katsura et al. (2002).
The type strain is NBRC 3267T (= ATCC 19441T = DSM 9533T = DSM 9534T = LMG 1368T = NRRL B-4241T), isolated from cherry (Prunus sp.). The DNA G+C content of the type strain is 55.9 mol%.
1.4.2.3 Gluconobacter frateurii Mason and Claus 1989
For the characteristics of the species, refer to Mason and Claus (1989).
The type strain is Kondo 40T (= NBRC 3264T = ATCC 49207T = DSM 7146T = LMG 1365T), isolated from strawberry (Fragaria ananassa). The DNA G+C content of the type strain is 55.1 mol%.
1.4.2.4 Gluconobacter albidus (ex Kondo and Ameyama 1958) Yukphan, Takahashi, Potacharoen, Tanasupawat, Nakagawa, Tanticharoen and Yamada 2005
For the characteristics of the species, refer to Yukphan et al. (2004a).
The type strain is NBRC 3250T (= BCC 14434T = JCM 20271T), isolated from a flower of dahlia by Kondo and Ameyama (1958). The DNA G+C content of the type strain is 60.0 mol%.
1.4.2.5 Gluconobacter thailandicus Tanasupawat, Thawai, Yukphan, Moonmangmee, Itoh, Adachi and Yamada 2005
For the characteristics of the species, refer to Tanasupawat et al. (2004).
The type strain is F-149-1T (= BCC 14116T = JCM 12310T = NBRC 100600T = TISTR 1533T), isolated from a flower of Indian cork tree (Millingtonia hortensis) Bangkok, Thailand. The DNA G+C content of the type strain is 55.8 mol%.
1.4.2.6 Gluconobacter kondonii Malimas, Yukphan, Takahashi, Kaneyasu, Potacharoen, Tanasupawat, Nakagawa, Tanticharoen and Yamada 2007
For the characteristics of the species, refer to Malimas et al. (2007).
The type strain is Kondo 75T (= BCC 14441T = NBRC 3266T), isolated from strawberry. The DNA G+C content of the type strain is 59.8 mol%.
1.4.2.7 Gluconobacter roseus (ex Asai 1935) Malimas, Yukphan, Takahashi, Muramatsu, Kaneyasu, Potacharoen, Tanasupawat, Nakagawa, Tanticharoen and Yamada 2008
For the characteristics of the species, refer to Malimas et al. (2008a).
The type strain is Asai G-2T (= BCC 14456T = JCM 20293T = NBRC 3990T), isolated from a fruit of kaki (persimmon, Diasporas kaki). The DNA G+C content of the type strain is 60.5 mol%.
1.4.2.8 Gluconobacter sphaericus (Ameyama 1975) Malimas, Yukphan, Takahashi, Muramatsu, Kaneyasu, Potacharoen, Tanasupawat, Nakagawa, Tanticharoen and Yamada 2008
Basonym: Gluconobacter oxydans subsp. sphaericus Ameyama 1975.
For the characteristics of the species, refer to Ameyama (1975) and Malimas et al. (2008b).
The type strain is NBRC 12467T (= BCC 14448T = LMG 1414T), isolated from fresh grapes by Ameyama (1975). The DNA G+C content of the type strain is 59.5 mol%.
1.4.2.9 Gluconobacter kanchanaburiensis Malimas, Yukphan, Lundaa, Muramatsu, Takahashi, Kaneyasu, Potacharoen, Tanasupawat, Nakagawa, Suzuki, Tanticharoen and Yamada 2009
For the characteristics of the species, refer to Malimas et al. (2009a).
The type strain is AD92T (= BCC 15889T = NBRC 103587T), isolated from a spoiled fruit of jackfruit (Artocarpus heterophyllus). The DNA G+C content of the type strain is 59.5 mol%.
1.4.2.10 Gluconobacter japonicus Malimas, Yukphan, Takahashi, Muramatsu, Kaneyasu, Potacharoen, Tanasupawat, Nakagawa, Tanticharoen and Yamada 2009
For the characteristics of the species, refer to Malimas et al. (2009b).
The type strain is Kondo 7T (= BCC 14458T = NBRC 3271T), isolated from a fruit of Chinese bayberry. The DNA G+C content of the type strain is 56.4 mol%.
1.4.2.11 Gluconobacter wancherniae Yukphan, Malimas, Lundaa, Muramatsu, Takahashi, Kaneyasu, Tanasupawat, Nakagawa, Suzuki, Tanticharoen and Yamada 2011
For the characteristics of the species, refer to Yukphan et al. (2010).
The type strain is AC42T (= BCC 15775T = NBRC 103581T), isolated from unknown seed. The DNA G+C content of the type strain is 56.6 mol%.
1.4.2.12 Gluconobacter uchimurae Tanasupawat, Kommanee, Yukphan, Moonmangmee, Muramatsu, Nakagawa and Yamada 2011
For the characteristics of the species, refer to Tanasupawat et al. (2011b).
The type strain is ZW160-2T (= BCC 14681T = NBRC 100627T), isolated from rakam fruit (Zalacca wallichiana). The DNA G+C content of the type strain is 60.5 mol%.
1.4.2.13 Gluconobacter nephelii Kommanee, Tanasupawat, Yukphan, Malimas, Muramatsu, Nakagawa and Yamada 2011
For the characteristics of the species, refer to Kommanee et al. (2011).
The type strain is RBY-1T (= BCC 36733T = NBRC 10606T), isolated from rambutan (Nephelium lappaceum). The DNA G+C content of the type strain is 57.2 mol%.
1.4.2.14 Gluconobacter cerevisiae Spitaels, Wieme, Balzarini, Cleenwerck, Van Landschoot, De Vuyst and Vandamme 2014
For the characteristics of the species, refer to Spitaels et al. (2014b).
The type strain is LMG 27748T (= DSM 27644T), isolated from fermenting lambic beer. The DNA G+C content of the type strain is 58.0 mol%.
1.4.3 Acidomonas Urakami, Tamaoka, Suzuki and Komagata 1989 emend. Yamashita, Uchimura and Komagata 2004
A.ci.do.mo’nas. L. adj. acidus, sour or acid; L. fem. n. monas, unit or monad; Acidomonas, acidophilic monad.
The genus Acidomonas was introduced for the facultatively methylotrophic bacterium, Acetobacter methanolicus Uhlig et al. 1986. However, the generic name was not accepted for a long time (Swings 1992; Sievers et al. 1994). The phylogenetic relationship between the genus Acidomonas and other genera of acetic acid bacteria was sufficiently remote to establish the new genus (Bulygina et al. 1992; Yamada et al. 1997; Yamashita et al. 2004).
Cells are gram negative, short rods, measuring 0.5–0.8 by 1.5–2.0 μm. Cells occur singly, in pairs, or rarely in short chains, and are either motile with a single polar flagellum or non motile. Colonies are shiny, smooth, circular, convex, entire, beige to pink, and 1–3 mm in diameter on glucose/peptone/yeast extract/malt extract (PYM) agar (pH 4.5) after 5 days at 30 °C. Pellicles are produced in PYM broth.
Aerobic. Catalase positive and oxidase negative. Acetic acid is produced from ethanol. Acetate is oxidized, but lactate is not or only weakly oxidized. Dihydroxyacetone is not produced from glycerol. d-Gluconate is produced from d-glucose. 2-Keto-d-gluconate, 5-keto-d-gluconate, or 2,5-diketo-d-gluconate is not produced in culture media. Acid is produced from l-arabinose, d-xylose, d-ribose, d-glucose, d-galactose, d-mannose, glycerol, ethanol, or methanol. Methanol, ethanol, d-glucose, d-mannose, glycerol, or succinic acid is utilized as a sole source of carbon. Pantothenic acid is essentially required for growth.
Grows on 30 % d-glucose (w/v) and 0.35 % acetic acid (v/v). Grows at pH 3.0. Grows at 30 °C but not at 45 °C. The major cellular fatty acids are C18:1ω7c, C16:0 and C18:12OH. The major quinone is Q-10. The DNA G+C content is 62–63 mol%. Strains of Acidomonas were abundantly isolated from activated sludges, except for the type strain, but not from vegetables, fruit, decayed wood and leaves, manure, and paddy soil. For more details of characteristics, see Komagata et al. (2014).
1.4.3.1 Acidomonas methanolica (Uhlig et al. 1986) Urakami, Tamaoka, Suzuki, and Komagata 1989 emend. Yamashita, Uchimura and Komagata 2004
Basonym: Acetobacter methanolicus Uhlig, Karbaum and Steudel 1986.
For the characteristics of the species, refer to Uhlig et al. (1986), Urakami et al. (1989), and Yamashita et al. (2004).
The type strain is MB 58T (= DSM 5432T = JCM 6891T = LMG 1668T = NRIC 0498T), isolated from a nonsterile fermentation process for the production of single-cell protein (SCP) from methanol with Candida species. The cells of the type strain are non motile, and the DNA G+C content is 62 mol%.
1.4.4 Gluconacetobacter corrig. Yamada, Hoshino and Ishikawa 1998
Glu.con.a.ce.to.bac’ter. N. L. neut. n. acetum gluconicum, gluconic acid; L. neut. n. acetum, vinegar; N. L. masc. n. bacter , rod; N. L. masc. n. Gluconacetobacter, gluconate-vinegar rod.
The genus Gluconacetobacter was introduced by the elevation of the subgenus Gluconacetobacter corrig. (ex Asai 1935) Yamada and Kondo 1984 for the Q-10-equipped Acetobacter species. Phylogenetically, the genus Gluconacetobacter consisted of two groups: the Gluconacetobacter liquefaciens group and the Gluconacetobacter xylinus group. For the latter group, the genus Komagataeibacter Yamada et al. 2013 was proposed.
Cells are gram negative rods, measuring 0.6–0.9 by 1.2–2.0 μm, with peritrichous flagella when motile, and occur singly or in pairs. Colonies are generally light brown to brown.
Aerobic. Catalase positive. Oxidase negative. Acid is produced from ethanol. Oxidizes acetate and lactate. Grows on glutamate agar and mannitol agar. A few species produce dihydroxyacetone from glycerol. 2-Keto-d-gluconate is produced from d-glucose. Most of species produce 2,5-diketo-d-gluconate, and a few species produce 5-keto-d-gluconate. Most of the species produce a water-soluble brown pigment. Acid is produced from l-arabinose, d-xylose, d-glucose, d-mannose, or ethanol. Grows on d-glucose, d-fructose, sucrose, d-mannitol, or ethanol. Ammoniac nitrogen is used as a sole nitrogen source. Strains of most species have the activity of nitrogen fixation.
Most of the species grow on 30 % d-glucose (w/v). Grows between 15 ° and 30 °C but not at 37 °C. The optimum growth temperature is around 30 °C. Grows at pH 3.0. The optimum growth pH is about 5.5. The major cellular fatty acid is C18:1ω7c. The major quinone is Q-10. The DNA G+C content is 58–65 mol%. For more details of characteristics, see Komagata et al. (2014).
The type species of the genus is Gluconacetobacter liquefaciens (Asai 1935) Yamada et al. 1998. Ten species are reported.
1.4.4.1 Gluconacetobacter liquefaciens (Asai 1935) Yamada, Hoshino and Ishikawa 1998
Basonym: Acetobacter aceti subsp. liquefaciens (Asai 1935) De Ley and Frateur 1974.
Synonyms: Acetobacter liquefaciens (Asai 1935) Gosselé, Swings, Kersters, Pauwels and De Ley 1983; ‘Gluconobacter liquefaciens’ Asai 1935.
For the characteristics of the species, refer to Asai et al. (1964), Gosselé et al. (1983b), Yamada and Kondo (1984), Navarro and Komagata (1999), Sievers and Swings (2005c), and Komagata et al. (2014).
The type strain is Asai G-1T (= ATCC 14835T = DSM 5603T = JCM 17840T = LMG 1381T = LMG 1382T = NBRC 12388T), isolated from dried persimmon. The DNA G+C content of the type strain is 64.9 mol%.
1.4.4.2 Gluconacetobacter diazotrophicus (Gillis et al. 1989) Yamada, Hoshino and Ishikawa 1998
Basonym: Acetobacter diazotrophicus Gillis, Kersters, Hoste, Janssens, Kroppenstedt, Stephan, Teixeira, Döbereiner and De Ley 1989.
For the characteristics of the species, refer to Gillis et al. (1989).
The type strain is Döbereiner PAl 5T (= ATCC 49037T = CCUG 37298T = CIP 103539T = DSM 5601T = LMG 7603T), isolated from roots and stems of sugarcane in Alagoas, Brazil. The DNA G+C content of the type strain is 61 mol%.
1.4.4.3 Gluconacetobacter sacchari Franke, Fegan, Hayward, Leonard, Stackebrandt and Sly 1999
For the characteristics of the species, refer to Franke et al. (1999).
The type strain is SRI 1794T (= CIP 106693T = DSM 12717T), isolated from the leaf sheath of sugarcane and from the pink sugarcane mealybug. The DNA G+C content of the type strain is 65 mol%.
1.4.4.4 Gluconacetobacter johannae Fuentes-Ramírez, Bustillos-Cristales, Tapia-Hernández, Jiménez-Salgado, Wang, Martínez-Romer and Caballero-Mellado 2001
For the characteristics of the species, refer to Fuentes-Ramírez et al. (2001).
The type strain is CFN-Cf55T (= ATCC 700987T = CIP 107160T = DSM 13595T), isolated from the rhizosphere of coffee plants. The DNA G+C content of the type strain is 57.96 mol%.
1.4.4.5 Gluconacetobacter azotocaptans Fuentes-Ramírez, Bustillos-Cristales, Tapia-Hernández, Jiménez-Salgado, Wang, Martínez-Romero and Caballero-Mellado 2001
For the characteristics of the species, refer to Fuentes-Ramírez et al. (2001).
The type strain is CFN-Ca54T (= ATCC 700988T = CIP 107161T = DSM 13594T), isolated from the rhizosphere of coffee plants. The DNA G+C content of the type strain is 64.01 mol%.
1.4.4.6 Gluconacetobacter tumulicola Tazato, Nishijima, Handa, Kigawa, Sano and Sugiyama 2012
For the characteristics of the species, refer to Tazato et al. (2012).
The type strain is K5929-2-1bT (= JCM 17774T = NCIMB 14760T), isolated from a black viscous substance in a plaster hole at the center of the ceiling in the stone chamber of the Kitora Tumulus in Asuka village, Nara Prefecture, Japan. The DNA G+C content of the type strain is 64.7 mol%.
1.4.4.7 Gluconacetobacter asukensis Tazato, Nishijima, Handa, Kigawa, Sano and Sugiyama 2012
For the characteristics of the species, refer to Tazato et al. (2012).
The type strain is K8617-1-1bT (= JCM 17772T = NCIMB 14759T), isolated from a brown viscous gel on the northeast area of the ceiling in the stone chamber of the Kitora Tumuli in Asuka village, Nara Prefecture, Japan. The DNA G+C content of the type strain is 65.4 mol%.
1.4.4.8 Gluconacetobacter tumulisoli Nishijima, Tazato, Handa, Tomita, Kigawa, Sano and Sugiyama 2013
For the characteristics of the species, refer to Nishijima et al. (2013).
The type strain is T611xx-1-4aT (= JCM 19097T = NCIMB 14861T), isolated from clay soil taken from near a spider web and an ant hole at a plugging stone directly under the plugging stone of the upper north side at the space adjacent to Takamatsuzuka Tumulus in Asuka village, Nara Prefecture, Japan. The DNA G+C content of the type strain is 66.5 mol%.
1.4.4.9 Gluconacetobacter takamatsuzukensis Nishijima, Tazato, Handa, Tomita, Kigawa, Sano and Sugiyama 2013
For the characteristics of the species, refer to Nishijima et al. (2013).
The type strain is T61213-20-1aT (= JCM 19094T = NCIMB 14859T), isolated from soil taken from the left side wall of the west side in the stone chamber exterior during the dismantling work of Takamatsuzuka Tumulus in Asuka village, Nara Prefecture, Japan. The DNA G+C content of the type strain is 66.6 mol%.
1.4.4.10 Gluconacetobacter aggeris Nishijima, Tazato, Handa, Tomita, Kigawa, Sano and Sugiyama 2013
For the characteristics of the species, refer to Nishijima et al. (2013).
The type strain is T6203-4-1aT (= JCM 19092T = NCIMB 14860T), isolated from soil taken from 5 cm below the surface in a bamboo grove of the burial mound of Takamatsuzuka Tumulus in Asuka village, Nara Prefecture, Japan. The DNA G+C content of the type strain is 65.4 mol%.
1.4.5 Asaia Yamada, Katsura, Kawasaki, Widyastuti, Saono, Seki, Uchimura and Komagata 2000
A.sa’i.a. N. L. fem. n. Asaia, Asai, named after Professor Toshinobu Asai, a Japanese bacteriologist who contributed to the systematics of acetic acid bacteria.
The strains of the genus Asaia were first found and isolated from flowers collected in Indonesia. In the beginning, the distribution of the Asaia strains was supposed to be restricted only to the tropical zone, that is, in Thailand, the Philippines, and Indonesia (Yamada and Yukphan 2008). However, the Asaia strains were isolated in the temperate zone, in Japan (Suzuki et al. 2010). The strains of the genus Asaia produced no or a very small amount of acetic acid from ethanol and did not grow in the presence of 0.35 % acetic acid (v/w).
Cells are gram negative, rod shaped, measuring 0.4–1.0 by 1.0–2.5 μm, and motile with peritrichous flagella. Colonies are smooth, entire, raised, shiny, and light brown, pink, to dark pinkish on glucose/peptone/yeast extract agar.
Aerobic. Catalase positive and oxidase negative. Produces no or a limited amount of acetic acid from ethanol. Oxidizes acetate and lactate to carbon dioxide and water. Grows on glutamate agar and mannitol agar. Dihydroxyacetone is generally produced. Produces 2-keto-d-gluconate and 5-keto-d-gluconate from d-glucose, but not 2,5-diketo-d-gluconate. Acid is produced from d-glucose, d-galactose, d-fructose, or other sugars and sugar alcohols. Grows on d-glucose, d-fructose, or d-mannitol. Ammoniac nitrogen is assimilated on d-glucose or d-mannitol.
Grows on 30 % d-glucose (w/v), but not in the presence of 0.35 % acetic acid (v/v). Growth generally occurs between 10 ° and 30 °C, but not at 37 °C. Grows at pH 3.0. The major cellular fatty acid is C18:1ω7c. The major quinone is Q-10. The DNA G+C content is 58.6–61.0 mol%. For more details of characteristics, see Komagata et al. (2014).
The type species of the genus is Asaia bogorensis Yamada et al. 2000. Eight species are reported.
1.4.5.1 Asaia bogorensis Yamada, Katsura, Kawasaki, Widyastuti, Saono, Seki, Uchimura and Komagata 2000
For the characteristics of the species, refer to Yamada et al. (2000).
The type strain is 71T (= JCM 19569T = NRIC 0311T), isolated from a flower of orchid tree (Bauhinia purpurea) in Bogor, Indonesia. The DNA G+C content of the type strain is 60.2 mol%.
1.4.5.2 Asaia siamensis Katsura, Kawasaki, Potacharoen, Saono, Seki, Yamada, Uchimura and Komagata 2001
For the characteristics of the species, refer to Katsura et al. (2001).
The type strain is S60-1T (= JCM 10715T = NBRC 16457T = NRIC 0323T), isolated from a flower of crown flower (Calotropis gigantea), in Bangkok, Thailand. The DNA G+C content of the type strain is 59.3 mol%.
1.4.5.3 Asaia krungthepensis Yukphan, Potacharoen, Tanasupapwat, Tanticharoen and Yamada 2004
For the characteristics of the species, refer to Yukphan et al. (2004b).
The type strain is AA08T (= BCC 12978T = NBRC 0535T = TISTER 1524T), isolated from a heliconia flower (Heliconia sp.) in Bangkok, Thailand. The DNA G+C content of the type strain is 60.3 mol%.
1.4.5.4 Asaia lannensis corrig. Malimas, Yukphan, Takahashi, Kaneyasu, Potacharoen, Tanasupawat, Nakagawa, Tanticharoen and Yamada 2008
For the characteristics of the species, refer to Malimas et al. (2008c).
The type strain is AB92T (= BCC 15733T = NBRC 102526T), isolated from a flower of spider lily (Crynum asiaticum) in Chiang Mai, Thailand. The DNA G+C content of the type strain is 60.8 mol%.
1.4.5.5 Asaia spathodeae Kommanee, Tanasupawat, Yukphan, Malimas, Muramatsu, Nakagawa and Yamada 2010
For the characteristics of the species, refer to Kommanee et al. (2010).
The type strain is GB23-2T (= BCC 36458T = NBRC 105894T = PCU 307T), isolated from a flower of the African tulip (Sapathodea campanulata) in Thailand. The DNA G+C content of the type strain is 59.7 mol%.
1.4.5.6 Asaia astilbis corrig. Suzuki, Zhang, Iino, Kosako, Komagata and Uchimura 2010
For the characteristics of the species, refer to Suzuki et al. (2010).
The type strain is T-6133T (= DSM 23030T = JCM 15831T), isolated from astilbe (Astilbe thunbergii var. congesta), Yamanashi, Japan. The DNA G+C content of the type strain is 58.9 mol%.
1.4.5.7 Asaia platycodi Suzuki, Zhang, Iino, Kosako, Komagata and Uchimura 2010
For the characteristics of the species, refer to Suzuki et al. (2010).
The type strain is T-683T (= JCM 25414T = DSM 23029T), isolated from balloon flower (Platycodon grandiflorum) in Akita, Japan. The DNA G+C content of the type strain is 60.0 mol%.
1.4.5.8 Asaia prunellae Suzuki, Zhang, Iino, Kosako, Komagata and Uchimura 2010
For the characteristics of the species, refer to Suzuki et al. (2010).
The type strain is T-153T (= DSM 23028T = JCM 25354T), isolated from self-heal (Prunella vulgaris) in Akita, Japan. The DNA G+C content of the type strain is 58.9 mol%.
1.4.6 Kozakia Lisdiyanti, Kawasaki, Widyastuti, Saono, Seki, Yamada, Uchimura and Komagata 2002
Ko.za’ki.a. N. L. fem. n. Kozakia, Kozaki, named after Professor Michio Kozaki, a Japanese bacteriologist who contributed to the study of microorganisms in tropical regions, especially Southeast Asia.
The genus Kozakia is phylogenetically related to the genus Asaia . However, the genus Kozakia especially differed from the genus Asaia in oxidation of ethanol to acetic acid and in production of a large amount of levan-like mucous substances from sucrose.
Cells are gram negative, rod shaped, and non motile, measuring 0.6–0.8 by 2.0–3.0 μm. Colonies are not pigmented.
Strictly aerobic. Catalase positive and oxidase negative. Acetic acid is produced from ethanol. Acetate and lactate are oxidized to carbon dioxide and water, but the activity is weak. Grows on mannitol agar but not on glutamate agar. Dihydroxyacetone is produced from glycerol. d-Gluconate, 2-keto-d-gluconate, and 5-keto-d-gluconate are produced from d-glucose, but 2,5-diketo-d-gluconate is not. A water-soluble brown pigment is not produced from d-glucose. Acid is produced from l-arabinose, d-xylose, d-glucose, d-galactose, d-mannose, melibiose, raffinose, meso-erythritol, glycerol, or ethanol. Methanol is not utilized. Ammoniac nitrogen is not assimilated on glucose, mannitol, or ethanol medium without vitamins. A levan-like mucous substance is produced from sucrose or d-fructose. γ-Pyrone is produced from d-fructose but not from d-glucose.
Growth is not inhibited by 0.35 % acetic acid (v/v) at pH 3.5. Does not grow on 30 % d-glucose (w/v). Grows at pH 3.0 and 30 °C. The major cellular fatty acid is C18:1ω7c. The major quinone is Q-10. The DNA G+C content is 56.8–57.2 mol%. For more details of characteritics, see Komagata et al. (2014).
1.4.6.1 Kozakia baliensis Lisdiyanti, Kawasaki, Widyastuti, Saono, Seki, Yamada, Uchimura and Komagata 2002
For the characteristics of the species, refer to Lisdiyanti et al. (2002).
The type strain is Yo-3T (= DSM 14400T = JCM 11301T = NBRC 16664T= NRIC 0488T), isolated from palm brown sugar collected in Bali, Indonesia in 1996. The DNA G+C content of the type strain is 57.2 mol%.
1.4.7 Swaminathania Loganathan and Nair 2004
Swa.mi.na.tha’ni.a. N. L. fem. n. Swaminathania, Swaminathan, named after Swaminathan, an Indian biologist, the father of the Green Revolution in India.
The strains of the genus Swaminathania, which were isolated using a nitrogen-free semisolid LGI medium at pH 5.5 from the rhizosphere, roots, and stems of salt-tolerant, mangrove-associated wild rice, were phylogenetically related especially to those of the genus Asaia . However, the genus was distinguished phenotypically from the genus Asaia by growth on 0.35 % acetic acid (v/v) and 3 % NaCl (w/v) or 1 % KNO3 (w/v).
Cells are gram negative, straight rods with round ends, measuring approximately 0.7–0.9 by 1.9–3.1 μm, and motile with peritrichous flagella. Colonies are initially yellowish and become dark orange later, smooth and raised, with entire margin on LGI medium.
Aerobic. Catalase positive and oxidase negative. Acetic acid is produced from ethanol under neutral and acidic conditions. Acetate and lactate are oxidized to carbon dioxide and water, but the activity was weak. Grows on mannitol agar and glutamate agar. Acid is produced from l-arabinose, d-glucose, d-galactose, d-mannose, d-sorbitol, glycerol, or ethanol. Methanol is not utilized. A water-soluble brown pigment is produced on glucose/calcium carbonate-containing agar. Strains are able to fix nitrogen. Solubilization of phosphate is shown. Grows intensely in the presence of 0.35 % acetic acid (v/v) at pH 3.5 and 3 % NaCl using 1 % KNO3 (w/v) as a nitrogen source.
The major cellular fatty acid is C18:1ω7c/ω9t/ω12t. The major quinone is Q-10. The DNA G+C content is 57.6–59.9 mol%. For more details of characteristics, see Komagata et al. (2014).
1.4.7.1 Swaminathania salitolerans Loganathan and Nair 2004
For the characteristics of the species, refer to Loganathan and Nair (2004).
The type strain is PA51T (= LMG 21291T = MTCC 3852T), isolated from mangrove-associated wild rice (Porteresia coarctata) in Pichavaram, Tamil Nadu, India. The DNA G+C content of the type strain is not reported.
1.4.8 Saccharibacter Jojima, Miura, Suzuki, Yokozeki, Yamanaka and Fudo 2004
Sac.cha.ri.bac’ter. L. neut. n. sacchrum or saccharon, sugar; N. L. masc. n. bacter, rod; N. L. masc. n. Saccharibacter, a sugar rod or a rod that grows intensely in a sugar-rich environment.
The strains of the genus Saccharibacter that were isolated from the pollen of a Japanese flower were quite remote phylogenetically from the strains of any other genera of acetic acid bacteria. The strains of the genus were osmophilic, showing no growth on 1 % glutamate agar (w/v) but growing on 7 % glutamate agar (w/v). The phylogenetically related genera are Neokomagataea , Swingsia , and Gluconobacter .
Cells are gram negative, straight rods, measuring 0.8–1.0 by 2.5–4.0 μm, and non motile. Colonies are circular, entire, and pale in color on yeast extract/glucose/peptone agar.
Strictly aerobic. Catalase positive and oxidase negative. Produces negligible or very little acetic acid from ethanol. Acetate is not oxidized to carbon dioxide and water, and lactate is weakly oxidized. Grows on mannitol agar and glutamate agar supplemented with 7 % substrates (w/v). Does not grow on common mannitol agar and glutamate agar with 1 % substrates (w/v). Dihydroxyacetone is not produced from glycerol. d-Gluconate, 2-keto-d-gluconate, and 5-keto-d-gluconate are produced from d-glucose. Acid is produced from l-arabinose, d-xylose, d-glucose, d-galactose, d-mannose, melibiose, sucrose, or d-mannitol. Methanol is not utilized. Ammoniac nitrogen is not assimilated on Hoyer–Frateur medium with d-glucose, d-mannitol, or ethanol. Cellulosic pellicles and water-soluble mucous substances are not produced. Not pigmented.
Grows in the glucose range between 2 % and 40 % (w/v), with an optimum around 10 % (w/v). High glucose concentration, for example, 10 % d-glucose (w/v), is preferable for growth. Osmophilic. No growth occurs in the presence of 0.35 % acetic acid (v/v) at pH 3.5. Temperature for growth ranges from 20 ° to 33 °C; the optimum is around 25–30 °C. The growth pH ranges from pH 4.0 to pH 7.5; the optimum pH is around pH 5.0 to pH 7.0. No growth is observed below pH 4.0. The major cellular fatty acids are C16:02OH (31.1–41.0 %) and C18:1ω7c (22.0–29.8 %). The major quinone is Q-10. The DNA G+C content is 52–53 mol%. For more details of characteristics, see Komagata et al. (2014).
1.4.8.1 Saccharibacter floricola Jojima, Mihara, Suzuki, Yokozeki, Yamanaka and Fudo 2004
For the characteristics of the species, refer to Jojima et al. (2004).
The type strain is S-877T (= AJ 13480T = DSM 15669T = JCM 12116T), isolated from pollen collected in Kanagawa Prefecture, Japan. The DNA G+C content of the type strain is 52.3 mol%.
1.4.9 Neoasaia Yukphan, Malimas, Potacharoen, Tanasupawat, Tanticharoen and Yamada 2006
Ne.o.a.sa’i.a. Gr. adj. neos, new; N. L. fem. n. Asaia , a bacterial name after Professor Asai, Japan; N. L. fem. n. Neoasaia, new Asaia.
The strain of the genus Neoasaia that was isolated from a flower of red ginger was closely related phylogenetically to those of the genera Kozakia, Asaia , and Swaminathania . However, the phenotypic characteristic was that of no oxidation of acetate and lactate, differentiating from the foregoing three genera.
Cells are gram negative, rod shaped, measuring 0.8–1.0 by 1.0–2.0 μm, and non motile. Colonies are smooth, raised, entire, shiny, and pink.
Aerobic. Acetic acid is produced from ethanol. Acetate and lactate are not oxidized. Grows on glutamate agar and mannitol agar. Dihydroxyacetone is weakly produced from glycerol. 2-Keto-d-gluconate and 5-keto-d-gluconate are produced from d-glucose. Acid is produced from d-arabinose weakly, l-arabinose, d-xylose, l-rhamnose weakly, d-fructose with delay, d-galactose, d-glucose, d-mannose, melibiose, sucrose, raffinose, d-mannitol weakly, d-sorbitol with delay, dulcitol weakly, meso-erythritol, glycerol, or ethanol.
Grows on d-arabinose weakly, l-arabinose, d-xylose, d-fructose, l-sorbose, d-galactose, d-glucose, d-mannose weakly, sucrose, raffinose, d-mannitol, d-sorbitol, dulcitol, meso-erythritol, or glycerol. Ammoniac nitrogen is hardly assimilated in the presence of d-glucose or d-mannitol as a carbon source. A water-soluble brown pigment is not produced on a glucose/peptone/yeast extract/calcium carbonate medium, and a levan-like polysaccharide is not produced on a sucrose medium. However, the production of fructan is reported by Neoasaia chiangmaiensis NBRC 101099T (Jacob et al. 2013).
Grows on 30 % d-glucose (w/v) and in the presence of 0.35 % acetic acid (v/v), but not in the presence of 1.0 % KNO3 (w/v). The major cellular fatty acid is C18:1ω7c. The major quinone is Q-10. The DNA G+C content is 63.1 mol%. For more details of characteristics, see Komagata et al. (2014).
1.4.9.1 Neoasaia chiangmaiensis Yukphan, Malimas, Potacharoen, Tanasupawat, Tanticharoen and Yamada 2006
For the characteristics of the species, refer to Yukphan et al. (2005).
The type strain is AC28T (= BCC 15763T = NBRC 101099T), isolated from a flower of red ginger (Alpinia purpurata) in Chiang Mai, Thailand, in September 2002. The DNA G+C content of the type strain is 63.1 mol%.
1.4.10 Granulibacter Greenberg, Porcella, Orcella, Stock, Wong, Conville, Murray, Holland and Zelazny 2006
Gra.nu.li.bac'ter. L. neut. n. granulum, grain; N. L. masc. n. bacter, rod; N. L. masc. n. Granulibacter, a rod that causes granules or granuloma formation.
The strain of the genus Granulibacter isolated first from three patients with chronic granulomatous disease was quite remote phylogenetically from other acetic acid bacteria. The strain grew at optimum temperatures of 35–37 °C and on methanol.
Gram negative, coccobacillus to rod shaped, and non motile. Colonies are convex, entire, smooth, and nondiffusible yellow on a modified glucose/yeast extract/calcium carbonate.
Strictly aerobic. Catalase positive and oxidase negative. Acetic acid is hardly produced from ethanol. Acetate and lactate are oxidized to carbon dioxide and water, but the activity of the former is weak. Grows on glutamate agar but weakly on mannitol agar. Dihydroxyacetone is not produced from glycerol. Acid is produced from d-glucose or ethanol and from glycerol weakly. Methanol can be used as a sole source of carbon. Ammoniac nitrogen is assimilated on glucose. A high concentration of d-glucose, for example, 5 % d-glucose (w/v), is preferable for growth.
Optimum temperature for growth is 35–37 °C. Optimum pH for growth is 5.0–6.5. Grows at pH 3.5. The major cellular fatty acids are C18:1ω7c and C16:0. The major quinone is Q-10 (Yukphan et al. 2009). The DNA G+C content is 59.1 mol%. For more details of characteristics, see Komagata et al. (2014).
1.4.10.1 Granulibacter bethesdensis Greenberg, Porcella, Stock, Wong, Conville, Murray, Holland and Zelazny 2006
For the characteristics of the species, refer to Greenberg et al. (2006).
The type strain is CGDNIH1T (= ATCC BAA-1260T = DSM 17861T), which was isolated from lymph node culture from a granulomatous disease patient in Bethesda, MD, USA, in 2003. The DNA G+C content of the type strain is 59.1 mol%.
1.4.11 Tanticharoenia Yukphan, Malimas, Muramatsu, Takahashi, Kaneyasu, Tanasupawat, Nakagawa, Suzuki, Potacharoen and Yamada 2008
Tan.ti.cha.ro.e'nia. N. L. fem. n. Tanticharoenia, named after Dr. Morakot Tanticharoen, Thailand, who contributed to studies of acetic acid bacteria.
The strains of the genus Tanticharoenia that were isolated from soil collected in Sakaerat, Nakhon Rachashima, Thailand, constituted an independent cluster phylogenetically. The strains did not oxidize either acetate or lactate, but grew on 30 % d-glucose (w/v).
Cells are gram negative, rod shaped, measuring 0.6–0.8 by 1.0–1.6 μm, and non motile. Colonies are creamy and smooth with entire margin when grown on glucose/ethanol/peptone/yeast extract/calcium carbonate agar.
Acetic acid is produced from ethanol. Acetate and lactate are not oxidized. Grows on glutamate agar weakly and on mannitol agar. Dihydroxyacetone is produced from glycerol. 2-Keto-d-gluconate, 5-keto-d-gluconate, and 2,5-diketo-d-gluconate are produced from d-glucose. A water-soluble brown pigment is intensely produced on glucose/peptone/yeast extract/calcium carbonate agar. Acid is produced from l-arabinose, d-xylose, d-fructose weakly, d-galactose, d-glucose, d-mannose, melibiose, sucrose weakly, raffinose weakly, meso-erythritol, glycerol, or ethanol. Grows on l-arabinose, d-xylose, d-fructose, d-glucose, d-galactose, meso-erythritol, d-mannitol, d-sorbitol, glycerol, or sucrose. Ammoniac nitrogen is not assimilated in the presence of d-glucose, d-mannitol, or ethanol as a carbon source.
Grows in the presence of 0.35 % acetic acid (v/v), but not of 1 % KNO3. Grows on 30 % d-glucose (w/v). The major cellular fatty acid is C18:1ω7c. The major quinone is Q-10. The DNA G+C content ranges from 64.5 to 65.6 mol%. For more details of characteristics, see Komagata et al. (2014).
1.4.11.1 Tanticharoenia sakaeratensis Yukphan, Malimas, Muramatsu, Takahashi, Kaneyasu, Tanasupawat, Nakagawa, Suzuki, Potacharoen and Yamada 2008
For the characteristics of the species, refer to Yukphan et al. (2008).
The type strain is AC37T (= BCC 15772T = NBRC 103193T), isolated from soil collected at Sakaerat, Nakhon Ratchasima, Thailand. The DNA G+C content of the type strain is 65.6 mol%.
1.4.12 Ameyamaea Yukphan, Malimas, Muramatsu, Takahashi, Kaneyasu, Potacharoen, Tanasupawat, Nakagawa, Hamana, Tahara, Suzuki, Tanticharoen and Yamada 2010
A.me.ya.ma’e.a. N. L. fem. n. Ameyamaea, Ameyama, named after Professor Minoru Ameyama, Japan, who contributed to studies of acetic acid bacteria, especially their biochemical and systematic studies.
The strains of the genus Ameyamaea that were isolated from flowers of red ginger collected in Chiang Mai, Thailand, were closely related phylogenetically to strains of the genus Tanticharoenia . However, the strains showed oxidation of acetate and weak oxidation of lactate and no growth on 30 % d-glucose (w/v), differing from those of the genus Tanticharoenia.
Cells are gram negative rods, measuring 0.6–0.8 by 1.0–1.8 μm, and motile with polar flagella. Colonies are creamy and smooth with entire margin on glucose/ethanol/peptone/yeast extract/calcium carbonate agar.
Acetic acid is produced from ethanol. Acetate is oxidized to carbon dioxide and water, but lactate is weakly oxidized. Grows on glutamate agar weakly and on mannitol agar. Dihydroxyacetone is weakly produced from glycerol. 2-Keto-d-gluconate and 5-keto-d-gluconate are produced from d-glucose. A water-soluble brown pigment is not produced on glucose/peptone/yeast extract/calcium carbonate agar.
Acid is produced from d-arabinose weakly, l-arabinose, d-xylose, l-rhamnose, d-glucose, d-mannose, d-galactose, meso-erythritol, glycerol weakly, melibiose, or ethanol. Grows on d-glucose, d-mannose very weakly, d-galactose, d-xylose, l-arabinose, l-rhamnose, d-fructose, l-sorbose, d-mannitol, d-sorbitol, dulcitol, meso-erythritol, glycerol, or melibiose very weakly. Growth is weak on methanol. Ammoniac nitrogen is very weakly assimilated in the presence of d-glucose, d-mannitol, or ethanol as a carbon source.
Grows in the presence of 0.35 % acetic acid (v/v), but not on 30 % d-glucose (w/v). The major cellular fatty acid is C18:1ω7c. The major quinone is Q-10. The DNA G+C content is 66.0–66.1 mol%. For more details of characteristics, see Komagata et al. (2014).
1.4.12.1 Ameyamaea chiangmaiensis Yukphan, Malimas, Muramatsu, Takahashi, Kaneyasu, Potacharoen, Tanasupawat, Nakagawa, Hamana, Tahara, Suzuki, Tanticharoen and Yamada 2010
For the characteristics of the species, refer to Yukphan et al. (2009).
The type strain is AC04T (= BCC 15744T = NBRC 103196T), isolated from a flower of red ginger (Alpinia purpurea) in Chiang Mai, Thailand. The DNA G+C content of the type strain is 66.0 mol%.
1.4.13 Neokomagataea Yukphan, Malimas, Muramatsu, Potacharoen, Tanasupawat, Nakagawa, Tanasupawat and Yamada 2011
Ne.o.ko.ma.ga.ta’ea. N. L. fem. n. Neokomagataea , new Komagata, named after Professor Kazuo Komagata, a Japanese microbiologist who contributed to bacterial systematics and phylogeny, especially of acetic acid bacteria.
The strains of the genus Neokomagataea that were isolated in Thailand from flowers of lantana and candle bush were related phylogenetically to those of the genus Gluconobacter . The strains of the genus grew on 30 % d-glucose (w/v) but not in the presence of 0.35 % acetic acid (v/v), the latter of which differed from those of the genus Gluconobacter.
Cells are gram negative rods, measuring 0.6–0.8 by 1.0–1.6 μm, and non motile. Colonies are smooth, entire, and creamy on glucose/ethanol/peptone/yeast extract/calcium carbonate agar.
Acetic acid is weakly produced from ethanol. Acetate and lactate are not oxidized. Grows on glutamate agar and mannitol agar. Dihydroxyacetone is not produced from glycerol. 2-Keto-d-gluconate, 5-keto-d-gluconate, and 2,5-diketo-d-gluconate are produced from d-glucose. A water-soluble brown pigment is not produced. Acid is produced from l-arabinose weakly, d-xylose, d-glucose, d-galactose weakly, d-fructose, or sucrose. Grows on d-glucose, l-rhamnose weakly, or sucrose. Ammoniac nitrogen is not generally assimilated on d-glucose or ethanol as a source of carbon.
Grows between 1.0 and 30 % d-glucose (w/v). Osmotolerant. Growth does not occur in the presence of 0.35 % acetic acid (v/v) or in the presence of 1.0 % or 2.0 % NaCl (w/v), or 1.0 % KNO3 (w/v). The major cellular fatty acids are C18:1ω7c, C16:0, and C18:12OH. The major quinone is Q-10. The DNA G+C content is 51.2–56.8 mol%. For more details of characteristics, see Komagata et al. (2014).
The type species of the genus is Neokomagataea thailandica Yukphan et al. 2011. Two species are reported.
1.4.13.1 Neokomagataea thailandica Yukphan, Malimas, Muramatsu, Potacharoen, Tanasupawat, Nakagawa, Tanticharoen and Yamada 2011
For the characteristics of the species, refer to Yukphan et al. (2011).
The type strain is AH11T (= BCC 25710T = NBRC 106555T), isolated from a flower of lantana (Lantana camera) at Tan Island, Hat Khanom-Mu Ko Thale Thai National Park, Nakhon-Si-Thammarat, Thailand, in 2007. The DNA G+C content of the type strain is 56.8 mol%.
1.4.13.2 Neokomagataea tanensis Yukphan, Malimas, Muramatsu, Potacharoen, Tanasupawat, Nakagawa, Tanticharoen and Yamada 2011
For the characteristics of the species, refer to Yukpohan et al. (2011).
The type strain is AH13T (= BCC 25711T = NBRC 106556T), isolated from a flower of candle bush (Senna alata) at Tan Island, Hat Khanom-Mu Ko Thale Thai National Park, Nakhon-Si-Thammarat, Thailand, in 2007. The DNA G+C content of the type strain is 51.2 mol%.
1.4.14 Komagataeibacter Yamada, Yukphan, Vu, Muramatsu, Ochaikul, Tanasupawat and Nakagawa 2013
Ko.ma.ga. ta.e.i.bac’ter. N. L. fem. n. Komagataea, Komagata, the name of a Japanese microbiologist; N. L. masc. n. bacter, rod; N. L. masc. n. Komagataeibacter, Komagata rod, which is named after Professor Kazuo Komagata, Japan, who contributed to the bacterial systematics, especially of acetic acid bacteria.
The genus Komagataeibacter was introduced for the Gluconacetobacter xylinus group of the genus Gluconacetobacter based on 16S rRNA gene sequence analysis and morphological, physiological, and ecological characterizations. The 11 species of the genus Gluconacetobacter were transferred to the genus Komagataeibacter as new combinations. Recently, three new combinations were additionally reported. The phenotypic characteristics of the genus Komagataeibacter were generally no motility, no production of 2,5-diketo-d-gluconate from d-glucose, and no water-soluble brown pigment production on glucose/peptone/yeast extract/calcium carbonate medium.
Cells are gram negative rods, measuring 0.5–0.8 by 1.0–3.0 μm, occurring singly, in pairs, or in chains. Non motile. Colonies are circular, smooth, or rough, raised to convex or umbonate, entire, glistening, and white-creamy to beige.
Aerobic. Catalase positive and oxidase negative. Acetic acid is produced from ethanol. Acetate and lactate are oxidized to carbon dioxide and water. Grows on glutamate agar and mannitol agar. Dihydroxyacetone is generally produced from glycerol. d-Gluconate, 2-keto-d-gluconate, and/or 5-keto-d-gluconate are produced from d-glucose, but 2,5-diketo-d-gluconate is not produced.
Acid is produced from l-arabinose, d-xylose, d-glucose, d-galactose, or ethanol. Grows on d-glucose, d-fructose, maltose, sucrose, or d-mannitol. Ammoniac nitrogen is generally assimilated on d-mannitol. Cellulosic materials are produced by some strains, that is, of Komagataeibacter xylinus and Komagataeibacter nataicola. A water-soluble brown pigment is not produced on glucose/yeast extract/calcium carbonate medium. γ-Pyrone compounds are not produced.
Grows generally in the presence of 0.35 % acetic acid (v/v). Some species require acetic acid for growth. Grows at pH 3.0. The major cellular fatty acid is C18:1ω7c. The major quinone is Q-10. The DNA G+C content ranges from 58 to 64 mol%. For more details of characteristics, see Komagata et al. (2014).
The type species of the genus is Komagataeibacter xylinus (Brown 1886) Yamada et al. 2013. Fourteen species are reported.
1.4.14.1 Komagataeibacter xylinus (Brown 1886) Yamada, Yukphan, Vu, Muramatsu, Ochaikul, Tanasupawat and Nakagawa 2013
Basonym: Acetobacter aceti subsp. xylinus corrig. (Brown 1886) De Ley and Frateur 1974.
Synonyms: Acetobacter xylinus (Brown 1886) Yamada 1984; Gluconacetobacter xylinus (Brown 1886) Yamada, Hoshino and Ishikawa 1998; ‘Bacterium xylinum’ Brown 1886.
For the characteristics of the species, refer to Gosselé et al. (1983b), Lisdiyanti et al. (2006), Navarro and Komagata (1999), Yamada (1983), Sievers and Swings (2005c), and Komagata et al. (2014).
The type strain is NCIMB 11664T (= DSM 6513T = JCM 7644T = LMG 1515T = NBRC 15237T = BCC 49175T), isolated from mountain ash berry by Professor G. Bertrand. The DNA G+C content of the type strain is 62.5 mol%.
1.4.14.2 Komagataeibacter hansenii (Gosselé et al. 1983) Yamada, Yukphan, Vu, Muramatsu, Ochaikul, Tanasupawat and Nakagawa 2013
Basonym: Acetobacter hansenii Gosselé, Swings, Kersters, Pauwels and De Ley 1983.
Synonyms: Gluconacetobacter hansenii (Gosselé et al. 1983) Yamada, Hoshino and Ishikawa 1998; Gluconacetobacter kombuchae Dutta and Gachhui 2007.
For the characteristics of the species, refer to Gosselé et al. (1983b), Lisdiyanti et al. (2006), Dutta and Chchhui (2007), Cleenwerck et al. (2009), Komagata et al. (2014), and Sievers and Swings (2005c).
The type strain is NCIMB 8746T (= DSM 5602T = JCM 7643T = LMG 1527T = NBRC 14820T = BCC 6318T), isolated from a local vinegar in Jerusalem, Israel. The DNA G+C content of the type strain is 59.0 mol%.
1.4.14.3 Komagataeibacter europaeus (Sievers et al. 1992) Yamada, Yukphan, Vu, Muramatsu, Ochaikul, Tanasupawat and Nakagawa 2013
Basonym: Acetobacter europaeus Sievers, Sellmer and Teuber 1992.
Synonym: Gluconacetobacter europaeus (Sievers et al. 1992) Yamada, Hoshino and Ishikawa 1998.
For the characteristics of the species, refer to Sievers et al. (1992).
The type strain is DES11T (= DSM 6160T = JCM 16935T = BCC 36446T), isolated from a submerged culture vinegar generator at a factory in Esslingen in the southern part of Germany. The DNA G+C content of the type strain is not described. The range of DNA G+C content is 56.2–57.3 mol%.
1.4.14.4 Komagataeibacter oboediens (Sokollek et al. 1998) Yamada, Yukphan, Vu, Muramatsu, Ochaikul, Tanasupawat and Nakagawa 2013
Basonym: Acetobacter oboediens Sokollek, Hertel and Hammes 1998.
Synonym: Gluconacetobacter oboediens (Sokollek et al. 1998) Yamada 2000.
For the characteristics of the species, refer to Sokollek et al. (1998) and Yamada (2000).
The type strain is LTH 2460T (= DSM 11826T = JCM 16937T = LMG 18849T = BCC 36445T), isolated from a submerged red wine vinegar fermentation at a factory in the southern part of Germany. The DNA G+C content of the type strain is 59.9 mol%.
1.4.14.5 Komagataeibacter intermedius (Boesch et al. 1998) Yamada, Yukphan, Vu, Muramatsu, Ochaikul, Tanasupawat and Nakagawa 2013
Basonym: Acetobacter intermedius Boesch, Trček, Sievers and Teuber 1998.
Synonym: Gluconacetobacter intermedius (Boesch et al. 1998) Yamada 2000.
For the characteristics of the species, refer to Boesch et al. (1998) and Yamada (2000).
The type strain is TF2T (= DSM 11804T = JCM 16936T = BCC 36447T = LMG 18909T), isolated from a commercially available tea fungus beverage (Kombucha) in Switzerland. The DNA G+C content of the type strain is 61.55 mol%.
1.4.14.6 Gluconacetobacter entanii Schüller, Hertel and Hammes 2000
For the characteristics of the species, refer to Schüller et al. (2000).
The type strain is LTH 4560T (= BCRC 17196T = DSM 13536T = LMG 20950T = LMG 21788T), isolated from submerged high-acid industrial vinegar fermentations. The DNA G+C content of the type strain is 58 mol%. The type strain is not available in any culture collections (Yamada et al. 2012b). This species is not listed as a new combination, according to Rule 27 of the Bacteriological Code (Tindall et al. 2006).
1.4.14.7 Komagataeibacter swingsii (Dellaglio et al. 2005) Yamada, Yukphan, Vu, Muramatsu, Ochaikul, Tanasupawat and Nakagawa 2013
Basonym: Gluconacetobacter swingsii Dellaglio, Cleenwerck, Felis, Engelbeen, Janssens and Marzotto 2005.
For the characteristics of the species, refer to Dellaglio et al. (2005).
The type strain is DST GL01T (= DSM 16373T = JCM 17123T = LMG 22125T = BCC 36451T), isolated from apple juice in South Tyrol region, Italy. The DNA G+C content of the type strain is 61.7 mol%.
1.4.14.8 Komagataeibacter rhaeticus (Dellaglio et al. 2005) Yamada, Yukphan, Vu, Muramatsu, Ochaikul, Tanasupawat and Nakagawa 2013
Basonym: Gluconacetobacter rhaeticus Dellaglio, Cleenwerck, Felis, Engelbeen, Janssens and Marzotto 2005.
For the characteristics of the species, refer to Dellaglio et al. (2005).
The type strain is DST GL02T (= DSM 16663T = JCM 17122T = LMG 22126T = BCC 36452T), isolated from apple juice in South Tyrol region, Italy. The DNA G+C content of the type strain is 63.4 mol%.
1.4.14.9 Komagataeibacter saccharivorans (Lisdiyanti et al. 2006) Yamada, Yukphan, Vu, Muramatsu, Ochaikul, Tanasupawat and Nakagawa 2013
Basonym: Gluconacetobacter saccharivorans Lisdiyanti, Navarro, Uchimura and Komagata 2006.
For the characteristics of the species, refer to Lisdiyanti et al. (2006).
The type strain is LMG 1582T (= BCC 36444T = JCM 25121T = NRIC 0614T = BCC 36444T), isolated from beet juice in Germany in 1927. The DNA G+C content of the type strain is 61 mol%.
1.4.14.10 Komagataeibacter nataicola (Lisdiyanti et al. 2006) Yamada, Yukphan, Vu, Muramatsu, Ochaikul, Tanasupawat and Nakagawa 2013
Basonym: Gluconacetobacter nataicola Lisdiyanti, Navarro, Uchimura and Komagata 2006.
For the characteristics of the species, refer to Lisdiyanti et al. (2006).
The type strain is LMG 1536T (= JCM 25120T = NRIC0616T = BCC 36443T), isolated from nata de coco in the Philippines. The DNA G+C content of the type strain is 62 mol%.
1.4.14.11 Komagataeibacter sucrofermentans (Toyosaki et al. 1996) Yamada, Yukphan, Vu, Muramatsu, Ochaikul, Tanasupawat and Nakagawa 2013
Basonym: Acetobacter xylinus subsp. sucrofermentans Toyosaki, Kojima, Tsuchida, Hoshino, Yamada and Yoshinaga 1996.
Synonym: Gluconacetobacter sucrofermentans (Toyosaki et al. 1996) Cleenwerck, De Vos and De Vuyst 2010.
For the characteristics of the species, refer to Toyosaki et al. (1995) and Cleenwerck et al. (2010).
The type strain is LMG 18788T (= DSM 15973T = JCM 9730T = BCC 7227T), isolated from a cherry. The DNA G+C content of the type strain is 62.7 mol%.
1.4.14.12 Komagataeibacter kakiaceti (Iino et al. 2012) Yamada 2014
Basonym: Gluconacetobacter kakiaceti Iino, Suzuki, Tanaka, Kosako, Ohkuma, Komagata and Uchimura 2012.
For the characteristics of the species, refer to Iino et al. (2012b) and Yamada (2014).
The type strain is G5-1T (= JCM 25156T = NRIC 0798T = LMG 26206T), isolated from kaki vinegar collected in Kumamoto Prefecture, Japan in 2005. The DNA G+C content of the type strain is 63.6 mol%.
1.4.14.13 Komagataeibacter medellinensis (Castro et al. 2013) Yamada 2014
Basonym: Gluconacetobacter medellinensis Castro, Cleenwerck, Trček, Zuluaga, De Vos, Caro, Aguirre, Putaux and Gañán 2013.
For the characteristics of the species, refer to Castro et al. (2013), Yamada et al. (1969b), and Yamada (2014).
The type strain is LMG 1693T (= NBRC 3288T = Kondo 51T), isolated from vinegar by K. Kondo, Japan. The DNA G+C content of the type strain is 60.7 mol%.
1.4.14.14 Komagataeibacter maltaceti (Slapšak et al. 2013) Yamada 2014
Basonym: Gluconacetobacter maltaceti Slapšak, Cleenwerck, De Vos and Trček 2013.
For the characteristics of the species, refer to Slapšak et al. (2013) and Yamada (2014).
The type strain is LMG 1529T (= NBRC 14815T = NCIMB 8752T), isolated from malt vinegar brewery acetifier by T.K. Walker in 1956. The DNA G+C content of the type strain is 62.5 mol%.
1.4.15 Endobacter Ramírez-Bahena, Teijedor, Martín, Velázquez and Peix 2013
En.do.bac’ter. Gr. pref. endo, within; N. L. masc. n. bacter, rod; N. L. masc. n. Endobacter, a rod isolated from the inside of a root nodule of alfalfa.
The strain of the genus Endobacter was isolated from a surface-sterilized nodule of alfalfa in Spain; it is quite remote phylogenetically from other acetic acid bacteria and constituted an independent cluster in a phylogenetic tree based on 16S rRNA gene sequences.
Cells are gram negative, coccoid to rod shaped. Motile with subpolar flagella. Colonies are white and mucoid on modified yeast extract mannitol agar.
Aerobic. Catalase positive and oxidase negative. Acetate and lactate are not oxidized. Acetic acid is produced from ethanol. Grows on glutamate agar and mannitol agar. Dihydroxyacetone is produced from glycerol. Acid is produced from d-xylose, d-glucose, glycerol, or ethanol. Grows between 20 ° and 37 °C with an optimum temperature for growth of 28 °C. Ammoniac nitrogen is assimilated on d-glucose. Optimal pH for growth ranges from 5.0 to 7.0, but growth occurs at pH 3.5.
The major cellular fatty acids are C18:1ω7c (39.94 %), C19:0cycloω8c (12.15 %), and C16:0 (13.40 %). The major quinone is Q-10. The DNA G+C content is 60.3 mol%. For more details of characteristics, see Komagata et al. (2014).
1.4.15.1 Endobacter medicaginis Ramírez-Bahena, Teijedor, Martín, Velázquez and Peix 2013
For the characteristics of the species, refer to Ramírez-Bahena (2013).
The type strain is M1MS02T (= CECT 8088T = LMG 26838T), isolated from a surface-sterilized nodule of alfalfa (Medicago sativa), Spain. The DNA G+C content of the type strain is 60.3 mol%.
1.4.16 Nguyenibacter Vu, Yukphan, Chaipitakchonlatarn, Malimas, Muramatsu, Bui, Tanasupawat, Duong, Nakagawa, Pham and Yamada 2013
Ngu.ye.ni.bac’ter. N. L. masc. n. Nguyenius, Nguyen, the name of a famous Vietnamese microbiologist; N. L. masc. n. bacter, rod; N. L. masc. n. Nguyenibacter, a rod, which is named after Professor Dung Lan Nguyen, Vietnam, who contributed to the study of microorganisms, especially of strains isolated in Vietnam.
The two strains of the genus Nguyenibacter were isolated by the use of nitrogen-free LGI medium. The strains were related phylogenetically to those of the genera Gluconacetobacter and Acidomonas .
Cells are gram negative rods, measuring 0.6–0.8 by 1.0–1.6 μm. Motile with peritrichous flagella. Colonies are smooth, entire, transparent, and creamy to brownish.
Aerobic. Catalase positive and oxidase negative. Acetic acid is not produced from ethanol. Acetate is oxidized to carbon dioxide and water, but lactate is not oxidized. Grows on glutamate agar and mannitol agar. Dihydroxyacetone is not produced from glycerol. 2-Keto-d-gluconate and 2,5-diketo-d-gluconate are produced from d-glucose. A water-soluble brown pigment is produced. Acid is produced from l-arabinose, d-xylose, d-glucose, d-galactose, d-fructose weakly, maltose, melibiose, sucrose, or raffinose weakly. Grows on d-glucose, d-galactose, l-arabinose weakly, d-xylose weakly, d-fructose weakly, l-sorbose weakly, maltose, melibiose weakly, sucrose, raffinose, d-mannitol weakly, d-sorbitol weakly, or glycerol. Ammoniac nitrogen is utilized on d-mannitol, but not on d-glucose or ethanol. Growth occurs on N2-free medium. γ-Pyrone compound is weakly produced. Levan-like polysaccharides are produced from sucrose.
Grows weakly on 30 % d-glucose (w/v), and weakly in the presence of 0.35 % acetic acid (v/v). Growth does not occur on 1.0 % KNO3 (w/v). The major cellular fatty acid is C18:1ω7c. The major quinone is Q-10. The DNA G+C content range is 68.1–69.4 mol%. For more details of characteristics, see Komagata et al. (2014).
1.4.16.1 Nguyenibacter vanlangensis Vu, Yukphan, Chaipitakchonlatarn, Malimas, Muramatsu, Bui, Tanasupawat, Duong, Nakagawa, Pham and Yamada 2013
For the characteristics of the species, refer to Vu et al. (2013).
The type strain is TN01LGIT (= BCC 54774T = NBRC 109046T = VTCC-B-1198T), isolated from the rhizosphere of Asian rice collected in Vietnam. The DNA G+C content of the type strain is 69.4 mol%.
1.4.17 Swingsia Malimas, Chaipitakchonlatarn, Vu, Yukphan, Muramatsu, Tanasupawat, Potacharoen, Nakagawa, Tanticharoen and Yamada 2014
Swing’si.a. N. L. fem. n. Swingsia, Swings, named after Professor Jean Swings, Belgium, who contributed to the systematics of bacteria, especially of acetic acid bacteria.
The two strains of the genus Swingsia were isolated from flowers in Thailand and located at an intermediary position phylogenetically between the genera Gluconobacter and Neokomagataea . The strains grew on 30 % d-glucose (w/v) and at 37 °C, but not in the presence of 0.35 % acetic acid (v/v), and acetic acid was sometimes produced weakly from ethanol.
Cells are gram negative rods, measuring 0.6–0.8 by 1.0–1.8 μm. Non motile. Colonies are brownish and smooth with entire margin.
Aerobic. Catalase positive and oxidase negative. Acetic acid is produced weakly from ethanol. Acetate and lactate are not oxidized. Grows on glutamate agar and mannitol agar. Dihydroxyacetone is produced from glycerol. 2-Keto-d-gluconate, 5-keto-d-gluconate, and 2,5-diketo-d-gluconate are produced from d-glucose. A water-soluble pigment is produced. Acid is produced from l-arabinose weakly, d-arabinose weakly, d-xylose weakly, l-rhamnose weakly, d-glucose, d-mannose weakly, d-galactose, d-fructose weakly, d-arabitol weakly, d-mannitol, maltose weakly, lactose weakly, melibiose, sucrose, or raffinose weakly. Grows on d-glucose, d-fructose, sucrose, l-arabitol weakly, d-arabitol weakly, or d-mannitol. Ammoniac nitrogen is utilized on d-mannitol, but not on d-glucose or ethanol. Ammonic nitrogen is assimilated on d-mannitol but not on d-glucose or ethanol. Levan-like polysaccharides are not produced.
Grows on 30 % d-glucose (w/v), but not in the presence of 0.35 % acetic acid (v/v). Growth occurs in the presence of 1.0 % KNO3 (w/v). The major cellular fatty acid is C18:1ω7c. The major quinone is Q-10. The DNA G+C content range is 46.9–47.3 mol%.
1.4.17.1 Swingsia samuiensis Malimas, Chaipitakchonlatarn, Vu, Yukphan, Muramatsu, Tanasupawat , Potacharoen, Nakagawa, Tanticharoen and Yamada 2014
For the characteristics of the species, refer to Malimas et al. (2013).
The type strain is AH83T (= BCC 25779T = NBRC 107927T), isolated from a flower of golden trumpet. The DNA G+C content of the type strain is 46.9 mol%.
1.5 Genus and Species in Pseudacetic Acid Bacteria
Several strains were once isolated and named ‘Acetobacter aurantium’ by Kondo and Ameyama (1958). According to the description of the species, the strains were not able to oxidize acetate.
Asai et al. (1964) reinvestigated the strains for phenotypic characteristics and found that they had polar flagellation and oxidized acetate and lactate to carbon dioxide and water, and the strains were named the polarly flagellated intermediate strains. Additional three strains were then newly isolated and confirmed to have polar flagellation and the capability of oxidizing acetate and lactate (Ameyama and Kondo 1967).
In the isoprenoid quinone analysis of the polarly flagellated intermediate strains, Q-8 was detected as the major quinone, indicating that the quinone system obtained was quite different chemotaxonomically from either Q-9 of Acetobacter strains or Q-10 of Gluconobacter strains (Yamada et al. 1969a, 1976). In the cellular fatty acid composition of the polarly flagellated intermediate strains, iso-C15:0 acid was found as the major, indicating that the strains were quite different similarly from C18:1ω7c acid of Acetobacter and Gluconobacter strains (Yamada et al. 1981a).
For such unique bacterial strains equipped with Q-8 and iso-C15:0 acid, the name of pseudacetic acid bacteria was given (Yamada 1979; Yamada et al. 1981a, b; Lisdiyanti et al. 2003a). The genus Frateuria was later introduced for these strains by Swings et al. (1980). The genus is accommodated in the class Gammaproteobacteria Stackebrandt et al. 1988.
1.5.1 Frateuria Swings, Gillis, Kersters, De Vos, Gosselé and De Ley 1980 emend. Zhang, Liu and Liu 2011
Fra.teu’ri.a. N. L. fem. n. Frateuria , Frateur, named after Professor Joseph Frateur, Belgium, especially in recognition of the study of acetic acid bacteria.
The genus Frateuria was long thought to be monotypic. However, a second species was recently reported.
Cells are gram negative, rod shaped, measuring 0.4–0.8 by 0.8–2.0 μm, singly or in pairs, motile with a single polar or subpolar flagellum when motile. Shows luxuriant growth on glucose/yeast extract/calcium carbonate agar. Colonies are flat or circular, and medium turning to brown.
Aerobic. Catalase positive or negative. Oxidase negative or positive. Oxidizes lactate but not acetate (Swings et al. 1980; Lisdiyanti et al. 2003a). Grows on glutamate agar and mannitol agar. Dihydroxyacetone is generally produced from glycerol. Produces d-gluconate, 2-keto-d-gluconate, and 2,5-diketo-d-gluconate from d-glucose but not 5-keto-d-gluconate. Produces a water-soluble brown pigment. Acid is produced from d-arabinose, l-arabinose, d-ribose, d-xylose, l-rhamnose, d-galactose, d-glucose, d-mannose, d-fructose, glycerol, or ethanol. Does not grow on methanol. Assimilates ammoniac nitrogen on d-mannitol.
No growth is observed in the presence of 0.35 % acetic acid (v/v). Grows on 20 % d-glucose (w/v) at 34 °C and at pH 3.5. The major cellular fatty acid is iso-C15:0. The major quinone is Q-8. The DNA G+C content is 62–68 mol%. Frateuria strains are isolated from flowers of lily, rose, ladybell, and coconut and fruits of raspberry, mango, rambai, and jackfruit. For more details of characteristics, see Komagata et al. (2014) and Swings and Sievers (2005).
The type species is Frateuria aurantia (ex Kondo and Ameyama 1958) Swings et al. 1980. Two species are reported.
1.5.1.1 Frateuria aurantia (ex Kondo and Ameyama 1958) Swings, Gillis, Kersters, De Vos, Gosselé and De Ley 1980
Synonym: ‘Acetobacter aurantius’ corrig. Kondo and Ameyama 1958.
For the characteristics of the species, refer to Swings et al. (1980), Yamada et al. (1969a, 1976, 1981a, b), and Lisdiyanti et al. (2003a, b).
The type strain is G-6T (= Kondo 67T = NBRC 3245T = ATCC 33424T = DSM 6220T = LMG 1558T), isolated from a flower of lily. The DNA G+C content of the type strain is 65.0 mol%.
1.5.1.2 Frateuria terrea Zhang, Liu and Liu 2011
For the characteristics of the species, refer to Zhang et al. (2011).
The type strain is VA24T (= CGMCC 1.7053T = NBRC 104236T), isolated from forest soil of the Changbai Mountains, Heilongjiang Province, China. The DNA G+C content of the type stran is 67.4 mol%.
1.6 Closing Remarks
More than 100 years have already passed since the genus Acetobacter Beijerinck 1898 was introduced with the only known species, Acetobacter aceti (Pasteur 1864) Beijerinck 1898, for vinegar-producing acetic acid bacteria. Up to 1960, acetic acid bacteria were believed to constitute a quite small taxonomic group, that is, only the one genus. However, their circumstances have entirely changed.
Acetic acid bacteria, including the vinegar-producing bacteria and their relatives, have been found in large numbers by expanding their possible living environments, viz., activated sludge, rhizosphere soils, soils, pollen, human patients, mosquitoes, stone chambers of tumuluses, and nodules of plants, in addition to sugary and alcoholic materials.
Up to the present, the acetic acid bacteria have numbered 17 genera and 84 species. These numbers will be greatly increased in future, pushing back the frontiers of their living environments and their isolation sources, and many new taxa, that is, new genera and new species, will be reported.
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The author expresses his thanks to Huong Thi Lan Vu, Vietnam, for constructing a phylogenetic tree.
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Yamada, Y. (2016). Systematics of Acetic Acid Bacteria. In: Matsushita, K., Toyama, H., Tonouchi, N., Okamoto-Kainuma, A. (eds) Acetic Acid Bacteria. Springer, Tokyo. https://doi.org/10.1007/978-4-431-55933-7_1
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