Abstract
Hodgkin’s disease differs from non-Hodgkin’s lymphomas by the presence of reactive lymphocytes, histiocytes, plasma cells, fibroblasts, and eosinophils in addition to the abnormal, so-called Reed-Sternberg cells and their variants. Usually, Reed-Sternberg cells constitute only a minor population, whereas there is a majority of small reactive lymphocytes. Non-Hodgkin’s lymphomas have been demonstrated by immunological or gene analysis approaches to be monoclonal populations of B- or T-lymphocyte-derived cells. In Hodgkin’s disease neither the cell of origin nor the monoclonal origin of Reed-Sternberg cells has been established. One approach to analyze the origin and nature of Reed-Sternberg cells is the establishment of cell lines derived from tissues or fluids involved in Hodgkin’s disease. Ideally, one should be able to demonstrate identical membrane and cytoplasmic markers, chromosomal abnormalities, and gene rearrangements in Reed-Sternberg cells in tissue sections and in the in vitro counterparts. Here we will describe the establishment and characterization of cell line ZO, derived from a pericardial effusion in a patient with the nodular sclerosis type of Hodgkin’s disease. In addition we will describe the preparation of three new antibodies against this cell line and report their staining patterns and those of other anti-Reed-Sternberg cell reagents on Hodgkin’s and non-Hodgkin’s cell lines and Hodgkin’s and non-Hodgkin’s lymphomas.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
Similar content being viewed by others
References
Brinker MGL, Poppema S, Buys CHCM, Timens W, Osinga J, Visser L (1987) Clonal immunoglobulin gene rearrangements in tissues involved by Hodgkin’s disease. Blood 70: 186–191
Diehl V, Kirchner HH, Schaadt M, Fonatsch C, Stein H, Gerdes J, Boie C (1981) Hodgkin’s disease: establishment and characterization of four in vitro cell lines. J Cancer Res Clin Oncol 101: 111–124
Jones DB, Scott CS, Wright DH, Stein H, Beverly PCL, Payne SV, Crawfords DH (1985) Phenotypic analysis of an established cell line derived from a patient with Hodgkin’s disease. Hematol Oncol 3: 133–145
Kamesaki H, Fukuhara S, Tatsumi E, Uchino H, Yamabe H, Miwa H, Shikakawa S, Hatanaka M, Honju T (1986) Cytochemical, immunologic, chromosomal and molecular genetic analysis of a novel cell line derived from Hodgkin’s disease. Blood 68: 285–292
Poppema S, de Jong B, Atmosoerodjo J, Idenburg V, Visser L, de Leij L (1985) Morphological, immunological, enzymehistochemical and chromosomal analysis of a cell line derived from Hodgkin’s disease. Cancer 55: 683–690
Schaadt M, Diehl V, Stein H, Fonatsch C, Kirchner HH (1980) Two neoplastic cell lines with unique features derived from Hodgkin’s disease. Int J Cancer 26: 723–731
Timens W, Brinker M, Osinga J, Buys CHCM, Visser L, Halie MR, Poppema S (1985) Ig gene rearrangement in Hodgkin’s disease cell line. Exp Hematol 13: 421
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1989 Springer-Verlag Berlin · Heidelberg
About this paper
Cite this paper
Poppema, S., Visser, L., de Jong, B., Brinker, M., Atmosoerodjo, J., Timens, W. (1989). The Typical Reed-Sternberg Phenotype and I g Gene Rearrangement of Hodgkin’s Disease Derived Cell Line ZO Indicating a B-Cell Origin. In: Diehl, V., Pfreundschuh, M., Loeffler, M. (eds) New Aspects in the Diagnosis and Treatment of Hodgkin’s Disease. Recent Results in Cancer Research, vol 117. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-83781-4_7
Download citation
DOI: https://doi.org/10.1007/978-3-642-83781-4_7
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-83783-8
Online ISBN: 978-3-642-83781-4
eBook Packages: Springer Book Archive