Abstract
In angiosperm plants the interaction between male and female gametophytes, the pollen tube and the embryo sac respectively, results in double fertilization. The pollen tube delivers two male gametes, the sperm cells, into the embryo sac. One sperm cell fuses with the egg cell to form the embryo, and the second sperm cell fuses with the central cell to form the endosperm. Most of our knowledge of the fertilization process is based on microscopical observations of fixed and sectioned materials (Jensen 1972; Van Went and Willemse 1984; Willemse and Van Went 1984). The embryo sac and the fertilization events taking place within it, cannot be observed directly, because it is positioned deeply inside the ovule, surrounded by a large quantity of untransparant sporophytic tissues.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
Similar content being viewed by others
References
Dupuis I, Roeckel P, Matthys-Rochon E, Dumas C (1987) Procedure to isolate viable sperm cells from corn (Zea mays L.) pollen grains Plant Physiol 85: 876–878
Heslop-Harrison J, Heslop-Harrison Y (1989) Myosin associated with the surfaces of organelles, vegetative nuclei and generative cells in angiosperm pollen grains and tubes. J Cell Sci 94: 319–325
Hu S-Y, Li L-G, Zhou C (1985) Isolation of viable embryo sacs and their protoplasts of Nicotiana tabacum. Acta Bot Sin 27: 337–344
Huang B-Q, Russell SD (1989) Isolation of fixed and viable eggs, central cells and embryo sacs from ovules of Plumbago zeylanica. Plant Physiol 90: 9–12
Jensen WA (1964) Observations on the fusion of nuclei in plants. J Cell Biol 23: 669–672
Jensen WA (1972) The embryo sac and fertilization in angiosperms. Harold L. Lyon Arbor Lect 3: 1–32
Keijzer CJ, Wilms HJ, Mogensen HL (1988) Sperm cell research: the current status and applications for plant breeding. In: Wilms HJ, Keijzer CJ (eds) Plant sperm cells as tools for biotechnology. Pudoc, Wageningen, pp 3–8
Kirk JTO, Tilney-Bassett RAE (1978) The plastids: their chemistry, structure, growth and inheritance. Elsevier/North Holland Biomed Press, Amsterdam
Kranz E, Bautor J, Lörz H (1991) In vitro fertilization of single, isolated gametes of maize mediated by electrofusion. Sex Plant Reprod 4: 12–16
Meyer B, Stubbe W (1974) Das Zahlenverhaltnis von mutterlichen und vaterlichen Plastiden in den Zygoten von Oenothera erythrosepala Borbas (syn. Oe. Lamarckiana). Ber Dtsch Bot Ges 87: 29–38
Mogensen HL (1982) Double fertilization in barley and the cytological explanation for haploid embryo formation, embryoless caryopses, and ovule abortion. Carlsberg Res Commun 47: 313–354
Mogensen HL (1988) Exclusion of male mitochondria and plastids during syngamy in barley as a basis for maternal inheritance. Proc Natl Acad Sci, USA 85: 2594–2597
Mol R (1986) Isolation of protoplasts from female gametophytes of Torenia fournieri. Plant Cell Rep 3: 202–206
Roman H (1948) Directed fertilization in maize. Proc Natl Acad Sci, USA 34: 46–52
Russell SD (1982) Fertilization in Plumbago zeylanica: entry and discharge of the pollen tube in the embryo sac. Can J Bot 60: 2219–2230
Russell SD (1983) Fertilization in Plumbago zeylanica: gametic fusion and fate of the male cytoplasm. Amer J Bot 70: 416–434
Russell SD (1985) Preferential fertilization in Plumbago: ultrastructural evidence for gamete-level recognition in an angiosperm. Proc Natl Acad Sci, USA 82: 6129–6132
Russell SD (1986) A method for the isolation of sperm cells in Plumbago zeylanica. Plant Physiol 81: 317–319
Southworth D, Knox RB (1989) Flowering plant sperm cell: isolation from pollen of Gerbera jamesonii ( Asteraceae ). Plant Sci 60: 273–277
Theunis CH (1990) Ultrastructural analysis of Spinacia oleracea sperm cells isolated from mature pollen grains. Protoplasma 158: 176–181
Theunis CH, Van Went JL (1989) Isolation of sperm cells from mature pollen grains of Spinacia oleracea L. Sex Plant Reprod 2: 97–102
Theunis CH, Pierson ES, Cresti M (1991) Isolation of male and female gametes in higher plants. Sex Plant Reprod 4: 145–154
Van Aelst AC, Theunis CH, Van Went JL (1990) Freeze-fracture studies on isolated sperm cells of Spinacia oleracea L. Protoplasma 153: 204–207
Van Went JL, Kwee H-S (1990) Enzymatic isolation of living embryo sacs of Petunia. Sex Plant Reprod 3: 257–262
Van Went JL, Willemse MTM (1984) Fertilization. In: Johri BM (ed) Embryology of angiosperms. Springer, Berlin Heidelberg New York Tokyo, pp 273–317
Wagner VT, Kardolus JP, Van Went JL (1989) Isolation of the lily embryo sac. Sex Plant Reprod 2: 219–224
Willemse MTM, Van Went JL (1984) The female gametophyte. In: Johri BM (ed) Embryology of angiosperms. Springer, Berlin Heidelberg New York Tokyo, pp 159–196
Zhou C (1987) A study of fertilization events in living embryo sacs isolated from sunflower ovules. Plant Sci 52: 147–151
Zhou C, Yang HY (1986) Isolation of embryo sacs by enzymatic maceration and its potential in haploid study. In: Hu SY, Yang HY (eds) Haploids of higher plants in vitro. Springer, Berlin Heidelberg New York, pp 192–203
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1992 Springer-Verlag Berlin Heidelberg
About this paper
Cite this paper
van Went, J.L. (1992). Fertilization in Angiosperm Plants. In: Dattée, Y., Dumas, C., Gallais, A. (eds) Reproductive Biology and Plant Breeding. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-76998-6_18
Download citation
DOI: https://doi.org/10.1007/978-3-642-76998-6_18
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-77000-5
Online ISBN: 978-3-642-76998-6
eBook Packages: Springer Book Archive