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Fertilization in Angiosperm Plants

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Reproductive Biology and Plant Breeding

Abstract

In angiosperm plants the interaction between male and female gametophytes, the pollen tube and the embryo sac respectively, results in double fertilization. The pollen tube delivers two male gametes, the sperm cells, into the embryo sac. One sperm cell fuses with the egg cell to form the embryo, and the second sperm cell fuses with the central cell to form the endosperm. Most of our knowledge of the fertilization process is based on microscopical observations of fixed and sectioned materials (Jensen 1972; Van Went and Willemse 1984; Willemse and Van Went 1984). The embryo sac and the fertilization events taking place within it, cannot be observed directly, because it is positioned deeply inside the ovule, surrounded by a large quantity of untransparant sporophytic tissues.

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References

  • Dupuis I, Roeckel P, Matthys-Rochon E, Dumas C (1987) Procedure to isolate viable sperm cells from corn (Zea mays L.) pollen grains Plant Physiol 85: 876–878

    CAS  Google Scholar 

  • Heslop-Harrison J, Heslop-Harrison Y (1989) Myosin associated with the surfaces of organelles, vegetative nuclei and generative cells in angiosperm pollen grains and tubes. J Cell Sci 94: 319–325

    Google Scholar 

  • Hu S-Y, Li L-G, Zhou C (1985) Isolation of viable embryo sacs and their protoplasts of Nicotiana tabacum. Acta Bot Sin 27: 337–344

    Google Scholar 

  • Huang B-Q, Russell SD (1989) Isolation of fixed and viable eggs, central cells and embryo sacs from ovules of Plumbago zeylanica. Plant Physiol 90: 9–12

    Article  PubMed  CAS  Google Scholar 

  • Jensen WA (1964) Observations on the fusion of nuclei in plants. J Cell Biol 23: 669–672

    Article  PubMed  CAS  Google Scholar 

  • Jensen WA (1972) The embryo sac and fertilization in angiosperms. Harold L. Lyon Arbor Lect 3: 1–32

    CAS  Google Scholar 

  • Keijzer CJ, Wilms HJ, Mogensen HL (1988) Sperm cell research: the current status and applications for plant breeding. In: Wilms HJ, Keijzer CJ (eds) Plant sperm cells as tools for biotechnology. Pudoc, Wageningen, pp 3–8

    Google Scholar 

  • Kirk JTO, Tilney-Bassett RAE (1978) The plastids: their chemistry, structure, growth and inheritance. Elsevier/North Holland Biomed Press, Amsterdam

    Google Scholar 

  • Kranz E, Bautor J, Lörz H (1991) In vitro fertilization of single, isolated gametes of maize mediated by electrofusion. Sex Plant Reprod 4: 12–16

    Google Scholar 

  • Meyer B, Stubbe W (1974) Das Zahlenverhaltnis von mutterlichen und vaterlichen Plastiden in den Zygoten von Oenothera erythrosepala Borbas (syn. Oe. Lamarckiana). Ber Dtsch Bot Ges 87: 29–38

    Google Scholar 

  • Mogensen HL (1982) Double fertilization in barley and the cytological explanation for haploid embryo formation, embryoless caryopses, and ovule abortion. Carlsberg Res Commun 47: 313–354

    Article  Google Scholar 

  • Mogensen HL (1988) Exclusion of male mitochondria and plastids during syngamy in barley as a basis for maternal inheritance. Proc Natl Acad Sci, USA 85: 2594–2597

    Article  CAS  Google Scholar 

  • Mol R (1986) Isolation of protoplasts from female gametophytes of Torenia fournieri. Plant Cell Rep 3: 202–206

    Article  Google Scholar 

  • Roman H (1948) Directed fertilization in maize. Proc Natl Acad Sci, USA 34: 46–52

    Article  Google Scholar 

  • Russell SD (1982) Fertilization in Plumbago zeylanica: entry and discharge of the pollen tube in the embryo sac. Can J Bot 60: 2219–2230

    Article  Google Scholar 

  • Russell SD (1983) Fertilization in Plumbago zeylanica: gametic fusion and fate of the male cytoplasm. Amer J Bot 70: 416–434

    Article  Google Scholar 

  • Russell SD (1985) Preferential fertilization in Plumbago: ultrastructural evidence for gamete-level recognition in an angiosperm. Proc Natl Acad Sci, USA 82: 6129–6132

    Article  CAS  Google Scholar 

  • Russell SD (1986) A method for the isolation of sperm cells in Plumbago zeylanica. Plant Physiol 81: 317–319

    Article  PubMed  CAS  Google Scholar 

  • Southworth D, Knox RB (1989) Flowering plant sperm cell: isolation from pollen of Gerbera jamesonii ( Asteraceae ). Plant Sci 60: 273–277

    Google Scholar 

  • Theunis CH (1990) Ultrastructural analysis of Spinacia oleracea sperm cells isolated from mature pollen grains. Protoplasma 158: 176–181

    Article  Google Scholar 

  • Theunis CH, Van Went JL (1989) Isolation of sperm cells from mature pollen grains of Spinacia oleracea L. Sex Plant Reprod 2: 97–102

    Article  Google Scholar 

  • Theunis CH, Pierson ES, Cresti M (1991) Isolation of male and female gametes in higher plants. Sex Plant Reprod 4: 145–154

    Article  Google Scholar 

  • Van Aelst AC, Theunis CH, Van Went JL (1990) Freeze-fracture studies on isolated sperm cells of Spinacia oleracea L. Protoplasma 153: 204–207

    Article  Google Scholar 

  • Van Went JL, Kwee H-S (1990) Enzymatic isolation of living embryo sacs of Petunia. Sex Plant Reprod 3: 257–262

    Article  Google Scholar 

  • Van Went JL, Willemse MTM (1984) Fertilization. In: Johri BM (ed) Embryology of angiosperms. Springer, Berlin Heidelberg New York Tokyo, pp 273–317

    Google Scholar 

  • Wagner VT, Kardolus JP, Van Went JL (1989) Isolation of the lily embryo sac. Sex Plant Reprod 2: 219–224

    Article  Google Scholar 

  • Willemse MTM, Van Went JL (1984) The female gametophyte. In: Johri BM (ed) Embryology of angiosperms. Springer, Berlin Heidelberg New York Tokyo, pp 159–196

    Google Scholar 

  • Zhou C (1987) A study of fertilization events in living embryo sacs isolated from sunflower ovules. Plant Sci 52: 147–151

    Article  Google Scholar 

  • Zhou C, Yang HY (1986) Isolation of embryo sacs by enzymatic maceration and its potential in haploid study. In: Hu SY, Yang HY (eds) Haploids of higher plants in vitro. Springer, Berlin Heidelberg New York, pp 192–203

    Google Scholar 

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© 1992 Springer-Verlag Berlin Heidelberg

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van Went, J.L. (1992). Fertilization in Angiosperm Plants. In: Dattée, Y., Dumas, C., Gallais, A. (eds) Reproductive Biology and Plant Breeding. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-76998-6_18

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  • DOI: https://doi.org/10.1007/978-3-642-76998-6_18

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-77000-5

  • Online ISBN: 978-3-642-76998-6

  • eBook Packages: Springer Book Archive

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