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Metabolic Flux Analysis

  • Chapter
Bioreaction Engineering

Abstract

Metabolic pathways are sequences of enzyme-catalyzed reaction steps, converting substrates to a variety of products to meet the needs of the cell. Metabolic pathways can interact to create complex networks. Manipulation of metabolic pathways to improve the cellular properties is an old concept in biological sciences. This approach initially relied on random mutagenesis and creative selection techniques to identify superior strains with respect to a certain objective. Despite impressive successes in many biological areas, the mutations that played important role in achieving the desired cellular properties were poorly characterized and the strain development process remained random, combining science with elements of art [1]. Recombinant DNA technology introduced a new dimension to pathway manipulation, because it allowed precise modifications of enzymatic reactions in metabolic pathways. Metabolic engineering emerged then as the scientific field aiming at the directed modification of the enzymatic, regulatory, or transport activities of the cell to improve cellular properties, with the use of recombinant DNA technology [2-4].

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Authors
  1. Maria I. Klapa
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  2. Gregory Stephanopoulos
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Editors and Affiliations

  1. Institute of Technical Chemistry, University of Hannover, Callinstrasse 3, D-30167, Hannover, Germany

    Karl Schügerl  & Karl-Heinz Bellgardt  & 

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Klapa, M.I., Stephanopoulos, G. (2000). Metabolic Flux Analysis. In: Schügerl, K., Bellgardt, KH. (eds) Bioreaction Engineering. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-59735-0_4

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  • DOI: https://doi.org/10.1007/978-3-642-59735-0_4

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-64103-9

  • Online ISBN: 978-3-642-59735-0

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