Abstract
The cell division cycle is a coordinate and highly regulated series of events during which cells replicate their DNA content and then divide. Formally, the cell cycle has been divided in four phases: G1 (gap1 or presynthetic phase), S (synthetic phase), G2 (gap2 or postsynthetic phase) and M (mitosis). At the end of the mitotic events cells will choose between arrest (entering into the GO phase) or go through a new cell cycle. Each of these stages is characterized by a typical content of DNA: in G0 and in G1 phases cells have a diploid (2N) content of DNA, in G2 and M, they have a 4N DNA content, and in S phase they display an intermediate content of DNA.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
Similar content being viewed by others
References
Bartkova J, Lukas J, Strauss M, Bartek J (1994) Cell cycle-related variation and tissue-restricted expression of cyclin D1 protein. J Pathol, 172:237–245
Brons PP, Pennings AH, Haanen C, Wessels HM, Boezman JB (1990) Simultaneous measurement of DNA content and cell surface immunofluorescence of human bone-marrow cell using a single laser cytometer. Cytometry 11:837–844
Dolbeare F, Beisker W, Pallavicini MG, Vanderlaan M, Gray JW (1985) Cytochemistry for bromodeoxyuridine/DNA analysis: stoichiometry and sensitivity. Cytometry 6:521–530
Dolbeare F, Gratzner H, Pallavicini MG, Gray JW (1983) Flow cytometry measurement of total DNA content and incorporated bromodeoxyuridine. Proc Natl Acad USA 88: 5573–5577
Dressler LG, Bartow MA, Bartow SA (1989) DNA flow cytometry in solid tumors: practical aspects and clinical applications. Semin Diagnostic Pathol 6:55–82
Fox MHT, Arndt-Jovin MP, Baumann H, Robert-Nicoud M (1991) Spatial and temporal distribution of DNA replication sites localized by immunofluorescence and confocal microscopy in mouse fibroblasts. J Cell Sci 99: 247–253
Giaretti W, Nusse M, Brno S, Di Vinci A, Geido E (1989) A new method to discriminate G1, S, G2, M and G1 postmitotic cells. Exp Cell Res 182:290–295
Gong J, Traganos F, Darzynkiewicz Z (1994) Staurosporine blocks cell progression through G1 between the Cyclin D1 and Cyclin E restriction point. Cancer Res 54: 3136–3139
Jayat C, Ratinaud MH (1993) Cell Cycle analysis by flow cytometry: principles and applications. Biol Cell 78:15–25
Jianping G, Traganos F, Darzynkiewicz Z (1993) Expression of Cyclins B and E in individual MOLT-4 cells and in stimulated human lymphocytes during their progression through the cell cycle. Int J Oncol 3:1037–1042
Lu X, Lane PD (1993) Differential induction of transcriptionally active p53 following UV or ionizing radiation: defects in chromosome instability syndromes? Cell 75:765–778
Pagano M, Theodoras AM, Tam SW, Draetta G (1994) Cyclin D1-mediated inhibition of repair and replicative DNA synthesis in human fibroblasts. Genes Dev 8:1627–1639
Pucillo C, Salzano S, Pepe S, Vitale M, Formisano S, Rossi G (1990) Regulation of expression of low-affinity IgE receptor (FceRII) in the human monocyte-like cell line (U937) by phorbol esters and TPA. Int Arch Allergy Appl Immunol 93:330–337
Ronot X (1986) G2 arrest, binucleation, and single parameter DNA flow cytometer analysis. Cytometry 7:286–290
Schlüter C, Duchrow M, Wohlenberg C, Becker M, Key G, Flad H, Gerdes J (1993) The cell proliferation-associated antigen of antibody Ki-67: a very large, ubiquitous nuclear protein with numerous repeated elements, representing a new kind of cell cycle-maintaining proteins. J Cell Biol 123:513–522
Strong DM, Ahmed AA, Thurman GB, Sell KW (1973) In vitro stimulation of murine spleen cells using a microculture system and a multiple automated sample harvester. J Immunol Methods 2:279–287
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1996 Springer-Verlag Berlin Heidelberg
About this chapter
Cite this chapter
Giunta, M., Pucillo, C. (1996). Techniques for Monitoring Cell Cycle Phases. In: Pagano, M. (eds) Cell Cycle — Materials and Methods. Springer Lab Manual. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-57783-3_4
Download citation
DOI: https://doi.org/10.1007/978-3-642-57783-3_4
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-540-58066-9
Online ISBN: 978-3-642-57783-3
eBook Packages: Springer Book Archive