Abstract
Analysis of cellular DNA content and measurement of pulse-labeled newly replicated DNA by flow cytometry are useful techniques for cell cycle studies. In this chapter, we describe the protocols for cell cycle synchronization of mammalian cells, including time course designs and consideration of cell types to achieve successful experiments, along with the methods for detection of DNA. Some selected applications dealing with siRNA-mediated knockdown are also presented.
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Acknowledgments
This work was supported in part by The Naito Foundation Continuation Subsidy for Outstanding Projects (H.M.) and JSPS KAKENHI grant 23580243 (N.Y.-S.).
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Yoshizawa-Sugata, N., Masai, H. (2014). Cell Cycle Synchronization and Flow Cytometry Analysis of Mammalian Cells. In: Noguchi, E., Gadaleta, M. (eds) Cell Cycle Control. Methods in Molecular Biology, vol 1170. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-0888-2_13
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DOI: https://doi.org/10.1007/978-1-4939-0888-2_13
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