Skip to main content

Use of Immunohistochemistry in Cell Cycle Studies

  • Chapter
Cell Cycle — Materials and Methods

Part of the book series: Springer Lab Manual ((SLM))

  • 999 Accesses

Abstract

The recent enormous advances in the cell cycle research have been largely made through application of cell-free and cellular in vitro model systems. On the other hand, correlation with the situation in situ, i.e., in tissues and organs, is essential to help better understand the biology of the cell cycle and to use the knowledge gained so far for an initial phase of clinical applications.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Subscribe and save

Springer+ Basic
$34.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or eBook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 169.00
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 219.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Similar content being viewed by others

References

  • Bartkova J, Lukas J, Müller H, Lützhøft, D, Strauss M, Bartek J (1994a) Cyclin D1 protein expression and function in human breast cancer. Int J Cancer 57:353–361

    Article  PubMed  CAS  Google Scholar 

  • Bartkova J, Lukas J, Strauss M, Bartek J (1994b) Cell cycle-related variation and tissue-restricted expression of cyclin D1 protein. J Pathol 172:237–245

    Article  PubMed  CAS  Google Scholar 

  • Cattoretti G,. Pileri S, Parravicini C, et al. (1993) Antigen unmasking on formalin-fixed, paraffin-embedded tissue sections. J Pathol 171:83–98

    Article  PubMed  CAS  Google Scholar 

  • Doussis-Anagnostopoulou I, Tennant R, Gannon J, Gatter K (1994) Distribution of the cdc2 gene product in normal tissues: an immunological study using four new monoclonal antibodies. Histopathology (in press)

    Google Scholar 

  • Gillett C, Fantl V, Smith R, et al. (1994) Amplification and over-expression of cyclin D1 in breast cancer detected by immunohistochemical staining. Cancer Res 54:1812–1817

    PubMed  CAS  Google Scholar 

  • Hall PA, Levison DA, Wright NA (eds) (1992) Assessment of cell proliferation in clinical practice. Springer, Berlin Heidelberg New York

    Google Scholar 

  • Hall PA, Levison DA, Woods AL et al. (1990) Proliferating cell nuclear antigen (PCNA) immunolocalization in paraffin sections: an index of cell proliferation with evidence of deregulated expression in some neoplasms. J Pathol 162:285–294

    Article  PubMed  CAS  Google Scholar 

  • Harlow E, Lane DP (1988) Antibodies. A laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor

    Google Scholar 

  • Jiang W, Zhang, Y-J, Kahn SM et al. (1993) Altered expression of the cyclin D1 and reti-noblastoma genes in human esophageal cancer. Proc Natl Acad Sci USA 90:9026–9030

    Article  PubMed  CAS  Google Scholar 

  • Sawhney N, Hall P (1992) Ki-67 — structure, function, and new antibodies. J Pathol 168:161–162

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 1996 Springer-Verlag Berlin Heidelberg

About this chapter

Cite this chapter

Bartkova, J., Lukas, J., Bartek, J. (1996). Use of Immunohistochemistry in Cell Cycle Studies. In: Pagano, M. (eds) Cell Cycle — Materials and Methods. Springer Lab Manual. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-57783-3_1

Download citation

  • DOI: https://doi.org/10.1007/978-3-642-57783-3_1

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-540-58066-9

  • Online ISBN: 978-3-642-57783-3

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics