Abstract
High-throughput technologies of modern biology provide “molecular portraits” of tissues and have entered the field of oncology. In the present chapter, we describe tools of high-throughput expression analysis in transcriptomics and proteomics, with an emphasis on microarrays, two-dimensional electrophoresis, and mass spectrometry. Options and limitations in data production, extraction, and interpretation are outlined. Problems of sensitivity, specificity, multiple testing, and noise are discussed. As a concrete example, we review the application of these tools to the field of breast cancer, where expression analyses already contribute to individual treatment decisions.
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Kaindl, A., Oexle, K. (2009). Molecular Tools, Expression Profiling. In: Molls, M., Vaupel, P., Nieder, C., Anscher, M. (eds) The Impact of Tumor Biology on Cancer Treatment and Multidisciplinary Strategies. Medical Radiology. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-540-74386-6_17
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DOI: https://doi.org/10.1007/978-3-540-74386-6_17
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