Abstract
“Simple-Sequence-Repeats” (SSR) or “Microsatellites” are repeats of short sequence motifs with a length of 1 to 6 bp, which can be replicated up to 100 times (Tautz 1993) and occur in the non-coding regions of eucaryotic and chloroplast genomes in a very high diversity (Gupta et al. 1994). These sequence repeats are the main cause for the length polymorphisms of microsatellites in populations (Schlötterer and Tautz 1992) and are very useful for molecular taxonomy and population genetics (Zhivotovsky and Feldman 1995). Due to the large number of microsatellites in eukaryotes, and a high diversity within species or populations, they are the most important markers for genomic mapping and relation studies (Kashi et al. 1997; Queller et al. 1993). Trimer primers are widespread, give the most suitable results (Hughes and Queller 1993), and were used in many different studies before (Becher et al. 2000; Beyermann et al. 1992; Echt and May-Marquardt 1997; Echt et al. 1996; Jarret et al. 1997). Successful amplification of polymorphic banding pattern was possible with trinucleotide primers such as AAC7, AAG8, and GTG5 (Hale et al. 2001; Poulsen et al. 1993; Squirrell and Wolff 2001). Using AAC7 we were able to identify ecotypes of Brazil pine [Araucaria angustifolia (Bert.) O. Ktze.; Hampp et al. 2000].
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Vaasen, A., Scarano, F.R., Hampp, R. (2007). Population Biology of Different Clusia Species in the State of Rio de Janeiro. In: Lüttge, U. (eds) Clusia. Ecological Studies, vol 194. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-540-37243-1_8
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DOI: https://doi.org/10.1007/978-3-540-37243-1_8
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