Abstract
Beta-Lactamases OXA and CTX-M are highly disseminated and confer resistance to antibiotics. This project gathers, classifies and analyzes information from indexed articles and databases as UNIPROT, EMBL and PDB, in order to correlate molecular data with activity profile of beta-lactamases against beta-Lactamic antibiotics. Sequences of CTX-M and OXA enzymes are analyzed by ClustalW multiple alignment and distance trees are built by Neighbor – joining with default parameters and a Bootstrap of 1000, through MEGA 5.0.
Results from analysis were systematized with BLA.id system which documented 125 CTX-M, organized into four groups. Results show that CTX-M have punctual variations which change their hydrolytic activity profile. 310 OXAS grouped in 11 sets were analyzed and they show a high degree of conservation. Punctual changes in amino acids are correlated to changes in hydrolytic activity. BLA.id system fills a void in information about beta-Lactamases activity, which is disperse.
Access provided by Autonomous University of Puebla. Download to read the full chapter text
Chapter PDF
Similar content being viewed by others
References
Bush, K., Jacoby, G., Medeiros, A.: A Functional Classification Scheme for b-Lactamases and its Correlation with Molecular Structure. Antimicrobial Agents and Chemotherapy 39(6), 1211–1233 (1995)
Adriana, C.Y.: Descripción del ambiente genético del gen blaCTX-M_12 detectado en aislamientos de Klebsiella pneumoniae en hospitales de tercer nivel de (Tesis Maestría en Mi-crobiología), Universidad Nacional de Colombia. Facultad de Ciencias. Bogotá, 73 p. (2008)
Ângela, N., Iñaki, C., Fernando, B., Rafael, C., Teresa, M.C., Andrés, M., Fernando, G.-C., Juan, C.G.: Evolutionary Trajectories of Beta-Lactamase CTX-M-1 Cluster Enzymes: Predicting Antibiotic Resistance. PLoS Pathogens 6(1), 1–16 (2006)
del Carmen Rocha Gracia, R., Lozano P., Laguna, M.Y.: Mecanismos de Patogenicidad e Inter-acción: Parásito-Hospedero II, 245p. Benémerita Universidad Autónoma de Puebla, México (2006)
Sánchez, L., Ríos, R., Máttar, S.: Detección de beta-lactamasas de espectro exten-dido en Escherichia coli y Klebsiella pneumoniae aislados en una clínica de Villavicencio, Colombia. Asociacion Colombiana De Infectologia 12(3), 193–200 (2008)
Ambler, R.P., Coulson, A.F.W., Frére, J.-M., Ghuysen, J.M., Joris, B., Forsman, M., Lévesque, R.C., Tiraby, G., Waley, S.G.: A standard numbering scheme for the class A -lactamases. Biochem. J. 276, 269–272 (1991)
Emiliano, B.H., Reza, M.T.R.: Diseño de un modelo bioinformático para la detección, identificación y clasificación de genes codificantes de β-lactamasas,como herramienta para el cruce de datos moleculares con datos clínicos. Rev. Colomb. Cienc. Quím. Farm 36(2), 109–126 (2007)
Helena, H.Á.: Escherichia coli. productores de Blee aislados de urocultivo: implicaciones en el diagnóstico y tratamiento de la infección urinaria. Tesis. Universidad Complutense de Madrid (2010)
Villegas, M.V., Kattan, J.N., Correa, A., Lolans, K., Guzman, A.M., Woodford, N., Livermore, D., Quinn, J.P., The Colombian Nosocomial Bacterial Resistance Study Group.: Dissemination of Acinetobacter baumannii Clones with OXA-23 Carbapenemase in Colombian Hospitals. Antimicrobial Agents and Chemotherapy 51(6), 2001–2004 (2007)
Villegas, M., Correa, A., Pérez, F., Miranda, M., Zuluaga, T., Quinn, J.: Colombian Nosocomial Resistance Study Group. Prevalence and characterization of extended-spectrum beta-lactamases in Klebsiella pneumoniae and Escherichia coli isolates from Colombian hospital. Diagn. Microbiol. Infect. Dis. 49, 217–222 (2004)
ONU: Resistencia a los antimicrobianos: el drama del abuso. Boletín de la Organización Mun-dial de la Salud. 88, 797–876 (2010)
Resistencia a los antimicrobianos (RAM). Nota descriptiva N°194 (2012), http://www.who.int/mediacentre/factsheets/fs194/es/index.html
Sun, T., Nukaga, M., Mayama, K., Braswell, E.H., Knox, J.R.: Comparison of-lactamases of classes A and D: 1.5-Å crystallographic structure of the class D OXA-1 oxacillinase. Protein Science 12, 82–91 (2003)
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2014 Springer International Publishing Switzerland
About this paper
Cite this paper
Blanco, A.R.R., Reza, M.T.R., Barreto, E. (2014). Hydrolytic Activity of OXA and CTX-M beta-Lactamases against beta-Lactamic Antibiotics. In: Castillo, L., Cristancho, M., Isaza, G., Pinzón, A., Rodríguez, J. (eds) Advances in Computational Biology. Advances in Intelligent Systems and Computing, vol 232. Springer, Cham. https://doi.org/10.1007/978-3-319-01568-2_46
Download citation
DOI: https://doi.org/10.1007/978-3-319-01568-2_46
Publisher Name: Springer, Cham
Print ISBN: 978-3-319-01567-5
Online ISBN: 978-3-319-01568-2
eBook Packages: EngineeringEngineering (R0)