Abstract
Research and development in nanotechnology has become an increasingly popular trend in the last 5 years as the demand and production of nanometer-sized materials continue to grow. Nanotechnology is an area of research encompassing multidisciplinary studies (including chemistry, physics, engineering, and biotechnology), and has diverse applications in agriculture, automobile, clothing, defense and more recently, biology and biomedicine [1], [2]. Among many different nanotechnological products, quantum dots (QD) have gained a lot of popularity as imaging probes in biology due to their very special physico-chemical and optical properties [3], [4]. They are stable, highly fluorescent, tunable and can be functionalized via surface modifications. Despite the numerous ongoing studies on QD synthesis to improve their physical properties, the biological effects of QDs are poorly investigated. Thus far, it is known that QD biocompatibility is largely dependent on their size, surface charge, core and surface materials [2]. Currently, extensive studies on the interactions (or interference) of QDs with cellular processes are under investigation in many scientific centers.
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Keywords
- Nerve Growth Factor
- Glial Fibrillary Acidic Protein
- Extracellular Reactive Oxygen Species
- Glial Fibrillary Acidic Protein Promoter
- Cell Motility Assay
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
References
Leary SP, Liu CY, Apuzzo ML (2006) Toward the emergence of nanoneurosurgery: part II — nanomedicine: diagnostics and imaging at the nanoscale level. Neurosurgery 58: 805–823; discussion 805–823
Maysinger D (2008) Nanoparticles and cells: good companions and doomed partnerships. Org Biomol Chem 5: 2335–2342
Alivisatos AP, Gu W, Larabell C (2005) Quantum dots as cellular probes. Annu Rev Biomed Eng 7: 55–76
Zhang L, Gu FX, Chan JM, Wang AZ, Langer RS, Farokhzad OC (2007) Nanoparticles in medicine: therapeutic applications and developments. Clin Pharmacol Ther 83: 761–769
Zhang J, Campbell RE, Ting AY, Tsien RY (2002) Creating new fluorescent probes for cell biology. Nature Rev 3: 906–918
Lang P, Yeow K, Nichols A, Scheer A (2006) Cellular imaging in drug discovery. Nat Rev Drug Discov 5: 343–356
Whitesides GM (2003) The ‘right’ size in nanobiotechnology. Nat Biotechnol 21: 1161–1165
Bruchez M Jr, Moronne M, Gin P, Weiss S, Alivisatos AP (1998) Semiconductor nanocrystals as fluorescent biological labels. Science 281: 2013–2016
Chan WC, Nie S (1998) Quantum dot bioconjugates for ultrasensitive nonisotopic detection. Science 281: 2016–2018
Alivisatos P (2004) The use of nanocrystals in biological detection. Nat Biotechnol 22: 47–52
Chan WC, Maxwell DJ, Gao X, Bailey RE, Han M, Nie S (2002) Luminescent quantum dots for multiplexed biological detection and imaging. Curr Opin Biotechnol 13: 40–46
Jaiswal JK, Mattoussi H, Mauro JM, Simon SM (2003) Long-term multiple color imaging of live cells using quantum dot bioconjugates. Nat Biotechnol 21: 47–51
Larson DR, Zipfel WR, Williams RM et al (2003) Water-soluble quantum dots for multiphoton fluorescence imaging in vivo. Science 300: 1434–1436
Dubertret B, Skourides P, Norris DJ, Noireaux V, Brivanlou AH, Libchaber A (2002) In vivo imaging of quantum dots encapsulated in phospholipid micelles. Science 298: 1759–1762
Choi AO, Cho SJ, Desbarats J, Lovric J, Maysinger D (2007) Quantum dot-induced cell death involves Fas upregulation and lipid peroxidation in human neuroblastoma cells. Nanobiotechnol 5: 1–13
Zhang T, Stilwell JL, Gerion D et al (2006) Cellular effect of high doses of silica-coated quantum dot profiled with high throughput gene expression analysis and high content cellomics measurements. Nano Lett 6: 800–808
Lovric J, Bazzi HS, Cuie Y, Fortin GR, Winnik FM, Maysinger D (2005) Differences in subcellular distribution and toxicity of green and red emitting CdTe quantum dots. J Mol Med 83: 377–385
Nabiev I, Mitchell S, Davies A et al (2007) Nonfunctionalized nanocrystals can exploit a cell’s active transport machinery delivering them to specific nuclear and cytoplasmic compartments. Nano Lett 7: 3452–3461
Alberts B (2002) Molecular biology of the cell. Garland Science, New York, pp. xxxiv, 1463
Pathak S, Cao E, Davidson MC, Jin S, Silva GA (2006) Quantum dot applications to neuroscience: new tools for probing neurons and glia. J Neurosci 26: 1893–1895
Dahan M, Levi S, Luccardini C, Rostaing P, Riveau B, Triller A (2003) Diffusion dynamics of glycine receptors revealed by single-quantum dot tracking. Science 302: 442–445
Cui B, Wu C, Chen L et al (2007) One at a time, live tracking of NGF axonal transport using quantum dots. Proc Natl Acad Sci USA 104: 13666–13671
Bradbury EJ, McMahon SB, Ramer MS (2000) Keeping in touch: sensory neurone regeneration in the CNS. Trends Pharmacol Sci 21: 389–394
Guertin AD, Zhang DP, Mak KS, Alberta JA, Kim HA (2005) Microanatomy of axon/glial signaling during Wallerian degeneration. J Neurosci 25: 3478–3487
Heron PM, Sutton BM, Curinga GM, Smith GM, Snow DM (2007) Localized gene expression of axon guidance molecules in neuronal co-cultures. J Neurosci Meth 159: 203–214
Maysinger D, Behrendt M, Lalancette-Hebert M, Kriz J (2007) Real-time imaging of astrocyte response to quantum dots: in vivo screening model system for biocompatibility of nanoparticles. Nano Lett 7: 2513–2520
Williams K, Alvarez X, Lackner AA (2001) Central nervous system perivascular cells are immunoregulatory cells that connect the CNS with the peripheral immune system. Glia 36: 156–164
Sandros MG, Behrendt M, Maysinger D, Tabrizian M (2007) InGaP@ZnS-enriched chitosan nanoparticles: a versatile fluorescent probe for deep-tissue imaging. Adv Funct Mater 17: 3724–3730
Henry NL, Hayes DF (2006) Uses and abuses of tumor markers in the diagnosis, monitoring, and treatment of primary and metastatic breast cancer. Oncologist 11: 541–552
Heath JR, Davis ME (2008) Nanotechnology and cancer. Annu Rev Med 59: 251–265
Voura EB, Jaiswal JK, Mattoussi H, Simon SM (2004) Tracking metastatic tumor cell extravasation with quantum dot nanocrystals and fluorescence emission-scanning microscopy. Nat Med 10: 993–998
Stroh M, Zimmer JP, Duda DG et al (2005) Quantum dots spectrally distinguish multiple species within the tumor milieu in vivo. Nat Med 11: 678–682
Matsumura Y, Maeda H (1986) A new concept for macromolecular therapeutics in cancer chemotherapy: mechanism of tumoritropic accumulation of proteins and the antitumor agent smancs. Cancer Res 46: 6387–6392
Gao X, Cui Y, Levenson RM, Chung LW, Nie S (2004) In vivo cancer targeting and imaging with semiconductor quantum dots. Nat Biotechnol 22: 969–976
Anikeeva N, Lebedeva T, Clapp AR et al (2006) Quantum dot/peptide-MHC biosensors reveal strong CD8-dependent cooperation between self and viral antigens that augment the T cell response. Proc Natl Acad Sci USA 103: 16846–16851
Medintz IL, Clapp AR, Brunel FM et al (2006) Proteolytic activity monitored by fluorescence resonance energy transfer through quantum-dot-peptide conjugates. Nat Mater 5: 581–589
Pellegrino T, Parak WJ, Boudreau R et al (2003) Quantum dot-based cell motility assay. Differentiation 71: 542–548
GuW, Pellegrino T, Parak WJ et al (2005) Quantum-dot-based cell motility assay. Sci STKE 2005: l5
Gu W, Pellegrino T, Parak WJ et al (2007) Measuring cell motility using quantum dot probes. Methods Mol Biol 374: 125–131
Nie S, Xing Y, Kim GJ, Simons JW (2007) Nanotechnology applications in cancer. Annu Rev Biomed Eng 9: 257–288
Vicent MJ, Duncan R (2006) Polymer conjugates: nanosized medicines for treating cancer. Trends Biotechnol 24: 39–47
Duncan R (2006) Polymer conjugates as anticancer nanomedicines. Nat Rev Cancer 6: 688–701
Portney NG, Ozkan M (2006) Nano-oncology: drug delivery, imaging, and sensing. Anal Bioanal Chem 384: 620–630
Bagalkot V, Zhang L, Levy-Nissenbaum E et al (2007) Quantum dot-aptamer conjugates for synchronous cancer imaging, therapy, and sensing of drug delivery based on bi-fluorescence resonance energy transfer. Nano Lett 7: 3065–3070
Derfus AM, Chen AA, Min DH, Ruoslahti E, Bhatia SN (2007) Targeted quantum dot conjugates for siRNA delivery. Bioconjugate Chem 18: 1391–1396
Bakalova R, Ohba H, Zhelev Z, Ishikawa M, Baba Y (2004) Quantum dots as photosensitizers? Nat Biotechnol 22: 1360–1361
Samia AC, Dayal S, Burda C (2006) Quantum dot-based energy transfer: perspectives and potential for applications in photodynamic therapy. Photochem Photobiol 82: 617–625
Clarke SJ, Hollmann CA, Zhang Z et al (2006) Photophysics of dopamine-modified quantum dots and effects on biological systems. Nat Mater 5: 409–417
Hsieh JM, Ho ML, Wu PW, Chou PT, Tsai TT, Chi Y (2006) Iridium-complex modified CdSe/ZnS quantum dots; a conceptual design for bi-functionality toward imaging and photosensitization. Chem Commun 6: 615–617
Dayal S, Lou Y, Samia AC, Berlin JC, Kenney ME, Burda C (2006) Observation of non-Forstertype energy-transfer behavior in quantum dot-phthalocyanine conjugates. J Am Chem Soc 128: 13974–13975
Lovric J, Cho SJ, Winnik FM, Maysinger D (2005) Unmodified cadmium telluride quantum dots induce reactive oxygen species formation leading to multiple organelle damage and cell death. Chem Biol 12: 1227–1234
Ipe BI, Lehnig M, Niemeyer CM (2005) On the generation of free radical species from quantum dots. Small 1: 706–709
Samia AC, Chen X, Burda C (2003) Semiconductor quantum dots for photodynamic therapy. JAm Chem Soc 125: 15736–15737
Funnell WR, Maysinger D (2006) Three-dimensional reconstruction of cell nuclei, internalized quantum dots and sites of lipid peroxidation. J Nanobiotechnol 4: 1–19
Cho SJ, Maysinger D, Jain M, Roder B, Hackbarth S, Winnik FM (2007) Long-term exposure to CdTe quantum dots causes functional impairments in live cells. Langmuir 23: 1974–1980
Maysinger D, Lovric J, Eisenberg A, Savic R (2007) Fate of micelles and quantum dots in cells. Eur J Pharm Biopharm 65: 270–281
Ballou B, Lagerholm BC, Ernst LA, Bruchez MP, Waggoner AS (2004) Noninvasive imaging of quantum dots in mice. Bioconjugate Chem 15: 79–86
Fischer HC, Liu L, Pang KS, Chan WC (2006) Pharmacokinetics of nanoscale quantum dots: in vivo distribution, sequestration, and clearance in the rat. Adv Funct Mater 16: 1299–1305
Soo Choi H, Liu W, Misra P et al (2007) Renal clearance of quantum dots. Nat Biotechnol 25: 1165–1170
Lewinski N, Colvin V, Drezek R (2007) Cytotoxicity of nanoparticles. Small 4: 26–49
Nel A, Xia T, Madler L, Li N (2006) Toxic potential of materials at the nanolevel. Science 311: 622–627
Hardman R (2006) A toxicologic review of quantum dots: toxicity depends on physicochemical and environmental factors. Environ Health Perspect 114: 165–172
Choi AO, Brown SE, Szyf M, Maysinger D (2008) Quantum dot-induced epigenetic and genotoxic changes in human breast cancer cells. J Mol Med 86: 291–302
Evans JL, Goldfine ID, Maddux BA, Grodsky GM (2002) Oxidative stress and stress-activated signaling pathways: a unifying hypothesis of type 2 diabetes. Endocrine Rev 23: 599–622
Nagata S, Golstein P (1995) The Fas death factor. Science 267: 1449–1456
Green M, Howman E (2005) Semiconductor quantum dots and free radical induced DNA nicking. Chem Commun 1: 121–123
Callinan PA, Feinberg AP (2006) The emerging science of epigenomics. Human molecular genetics 15 Spec No 1: R95–R101
Sen T, Sen N, Tripathi G, Chatterjee U, Chakrabarti S (2006) Lipid peroxidation associated cardiolipin loss and membrane depolarization in rat brain mitochondria. Neurochem Int 49: 20–27
Ryman-Rasmussen JP, Riviere JE, Monteiro-Riviere NA (2007) Surface coatings determine cytotoxicity and irritation potential of quantum dot nanoparticles in epidermal keratinocytes. J Invest Dermatol 127: 143–153
Susumu K, Uyeda HT, Medintz IL, Pons T, Delehanty JB, Mattoussi H (2007) Enhancing the stability and biological functionalities of quantum dots via compact multifunctional ligands. J Am Chem Soc 129: 13987–13996
Zafarullah M, Li WQ, Sylvester J, Ahmad M (2003) Molecular mechanisms of N-acetylcysteine actions. Cell Mol Life Sci 60: 6–20
Ballou B, Ernst LA, Andreko S et al (2007) Sentinel lymph node imaging using quantum dots in mouse tumor models. Bioconjugate Chem 18: 389–396
van Engeland M, Nieland LJ, Ramaekers FC, Schutte B, Reutelingsperger CP (1998) Annexin V-affinity assay: a review on an apoptosis detection system based on phosphatidylserine exposure. Cytometry 31: 1–9
Boyles S, Lewis GP, Westcott B (1970) Intracellular enzymes in local lymph after chemical injury. Br J Pharmacol 38: 441P–442P
Darzynkiewicz Z, Bruno S, Del Bino G et al (1992) Features of apoptotic cells measured by flow cytometry. Cytometry 13: 795–808
O’Brien R, Gottlieb-Rosenkrantz P (1970) An automatic method for viability assay of cultured cells. J Histochem Cytochem 18: 581–589
O’Brien J, Wilson I, Orton T, Pognan F (2000) Investigation of the Alamar Blue (resazurin) fluorescent dye for the assessment of mammalian cell cytotoxicity. Eur J Biochem 267: 5421–5426
Mosmann T (1983) Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods 65: 55–63
Smith PJ, Blunt N, Wiltshire M et al (2000) Characteristics of a novel deep red/infrared fluorescent cell-permeant DNA probe, DRAQ5, in intact human cells analyzed by flow cytometry, confocal and multiphoton microscopy. Cytometry 40: 280–291
Budd SL, Castilho RF, Nicholls DG (1997) Mitochondrial membrane potential and hydroethidine-monitored superoxide generation in cultured cerebellar granule cells. FEBS Lett 415: 21–24
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Choi, A.O., Maysinger, D. (2008). Applications of quantum dots in biomedicine. In: Rogach, A.L. (eds) Semiconductor Nanocrystal Quantum Dots. Springer, Vienna. https://doi.org/10.1007/978-3-211-75237-1_12
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DOI: https://doi.org/10.1007/978-3-211-75237-1_12
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