Abstract
Taurine is actively transported into the renal tubular cell by a Na+ — and Cl−-dependent system specific for this β-amino acid and structural analogs (14). The proximal epithelial transport system regulates the urinary excretion of taurine. Taurine transport is regulated by the dietary intake of taurine in the rat (1), by extracellular taurine concentration in kidney cells in culture (7), and by medium osmolality in MDCK cells (11). We have previously demonstrated that taurine transport in both MDCK and LLC-PK1 cells is increased following incubation of cell monolayers in taurine-free medium and in decreased following incubation of cells in high extracellular taurine (8). The LLC-PK1 is a porcine cell line of proximal tubular origin and the MDCK (Madin Darby Canine Kidney) cell line is from the collecting duct of the dog.
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Chesney, R.W., Gusowski N., and Dabbaugh, S., 1985, Renal cortex taurine regulates the adaptive response to altered dietary intake of sulfur amino acids. J. Clin. Invest. 76:2213–2221.
Danzier, W.H., and Silbernagl, S., 1976, Renal tubular reabsorption of taurine, g-amino butyric acid (GABA) and β-alanine studied by continuous microperfusion. Pfluegers Arch. 367:123–128.
Garcia-Perez, A., Burg, M.B., 1991, Renal medullary organic osmolytes. Physiol. Rev. 71:1081–1115.
Heilig, C.W., Brenner, R.M., Yu, A.S.L., Kine, B.C., and Gullans, S.R., 1990, Modulation of osmolytes in MDCK cells by solutes, inhibitors and vasopressin. Am. J. Physiol. 259:F653–F659.
Huxtable RJ, 1992, Physiological actions of Taurine. Physiol. Rev. 72:101–163.
Jones, D.P., Miller, L.A., Dowling, C., Chesney, R.W., 1991, Regulation of Taurine transporter activity in LLC-PK1 cells: Role of protein synthesis and protein kinase C activation. J. Am. Soc. Nephrol. 2:1021–1029.
Jones, D.P., Miller, L.A., Chesney, R.W., 1993, Polarity of taurine transport in cultured renal epithelial cell lines: LLC-PK1 and MDCK. Am. J. Physiol. 265:F137–F145.
Moriyama, T., Garcia-Perez, A., Burg, M.B., 1990, Factors affecting the ratio of different organic osmolytes in renal medullary cells. Am. J. Physiol. 259:F847–F858.
Nakanishi, T., Balaban, R.S., Burg, M.B., 1988, Survey of osmolytes in renal cell lines. Am J Physiol 255: C181–C191.
Nakanishi, T., Turner, R.J., Burg, M.B., 1990, Osmoregulation of betaine transport in mammalian renal medullary cells. Am. J. Physiol. 258: F1061–1067.
Uchida, S., Kwon, H.M., Preston, A.S., and Handler, J.S., 1991, Taurine behaves as an osmolyte in MDCK cells: Protection by polarized, regulated transport of taurine. J. Clin. Invest. 88:656–662.
Uchida, S., Nakanishi, T., Kwon, H., Preston, A., Handler, J.S., 1991, Expression of MDCK cell Na and Cl dependent taurine transporter in Xenopus laevis oocytes. J. Biol. Chem. 266:9605–9609.
Yamamauchi A, Kwon, H.M., Uchida, S., Preston, A.S., Handler, J.S., 1991, Myo-inositol and betaine transporters regulated by tonicity are basolateral in MDCK cells. Am. J. Physiol. 261:F197–F202.
Zelikovic, I., Stejskal-Lorenz, E., Lohstroh, P. Budreau, A., and Chesney, R.W., 1989, Anion dependence of taurine transport by rat brush border membrane vesicles. Am J. Physiol. 256:F646–F6–55.
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© 1994 Springer Science+Business Media New York
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Jones, D.P., Jiang, B., Chesney, R.W. (1994). Regulation of Taurine Transport by External Taurine Concentration and Medium Osmolality in Renal Tubular Cells in Culture. In: Huxtable, R.J., Michalk, D. (eds) Taurine in Health and Disease. Advances in Experimental Medicine and Biology, vol 359. Springer, Boston, MA. https://doi.org/10.1007/978-1-4899-1471-2_14
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DOI: https://doi.org/10.1007/978-1-4899-1471-2_14
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