Abstract
The erythrocyte anion exchanger, or band 3 protein, catalyzes the exchange of chloride and bicarbonate in many cells. Anion exchanger activity is predominantly found in the basolateral membrane of the cortical collecting duct. Because proximal tubular renal taurine uptake across the brush border surface is dependent upon sodium and chloride, the effect of inhibitors of anion exchanger activity on renal taurine accumulation by rat renal brush border membrane vesicles was examined. The anion exchanger probes, DIDS (4,4-’diisothiosulfonic acid), and NBD-taurine (N-[7-nitrobenz-2-oxa-1,3-diazol-4-yl]-aminoethane sulfonic acid), and NBD-taurine (N-[7-nitrobenz-2-oxa-1,3-diazol-4-yl]-aminoethane sulfonic acid), were used as inhibitors of anion exchanger activity. DIDS, NAP-, and NBD-taurine all markedly inhibit the initial rate of NaCl-dependent accumulation of taurine by brush border membrane vesicles. NAP- and NBD-taurine accumulation is chloride dependent because inhibition was not found when uptake was performed in the presence of NaNO3 in place of chloride. In the presence of maximal inhibition of taurine uptake by NAP- or NBD-taurine, no additional inhibition was evident after incubation with DIDS. On the other hand, when a competitive inhibitor of taurine uptake, β-alanine, was used, additional inhibition of taurine accumulation was found in the presence of NAP- or NBD-taurine (p < .01 respectively). These results suggest some interaction of the anion exchanger and the taurine accumulation process at the apical surface of the proximal tubule. Because NBD-taurine is a fluorescent probe, it may be possible to isolate membrane peptides that bind NBD-taurine and demonstrate fluorescence. Preliminary isoelectric focusing experiments of brush border protein incubated with NBD-taurine show fluorescence localized to several particular fractions. Hence this observation that inhibitors of the anion exchanger system block NaCl-dependent taurine uptake can potentially serve as a means of isolating the taurine transporter protein.
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References
Booth, A.G, and Kenny, A.J., 1974, A rapid method for the preparation of microvilli from rabbit kidney, Biochem. J. 142:575–581.
Bradford, M., 1976, A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding, Anal. Biochem. 72:248–253.
Cabantchik, Z.I., Knauf, P.A., Ostwald, T., Markus, H., Davidson, L., Breuer, W., and Rothstein, A., 1976, The interaction of a photoreactive probe with the anion transport system of the human red blood cell, Biochim. Biophys. Acta 455:526–537.
Chesney, R.W., Gusowski, N., and Friedman, A.L., 1983, Renal adaptation to altered dietary sulfur amino acid intake occurs at the luminal brush border membrane, Kidney Int. 24:588–594.
Chesney, R.W., Sacktor, B., and Rowen, R.R., 1973, The binding of D-glucose to the isolated rabbit renal brush border, J. Biol. Chem. 218:2182–2191.
Daniel, W.D., 1983, Biostatistics: A foundation for analysis in the health sciences. Wiley, New York, pp 177–180.
Darmon, A., Eidelman, O., and Cabantchik, Z.I., 1982, A method for measuring anion transfer across membranes of hemoglobin-free cells and vesicles by continuous monitoring of fluoresence, Anal. Biochem. 119:313–321.
Dixon, T.F., and Purdom, M., 1954, Serum 5-nucleotidase, J. Clin. Path. 7:341–343.
Eidelman, O., Zangvill, M., Razin, M., Ginsburg, H., and Cabantchik, Z.I., 1981, The anion transfer system of erythrocyte membranes, Biochem. J. 195:503–513.
Goldinger, J.M., Khalsa, B.D.S., and Hong, S.K., 1984, Photoaffinity labeling of organic anion transport system in proximal tubule, Am. J. Physiol. 247:C217–227.
Goldinger, J.M., Khalsa, B.D.S., and Hong, S.K., 1984, Photoaffinity labeling of organic anion transport system in proximal tubule, Cell Physiol. 16:C217–227.
Knauf, P.A., Breuer, W., McCulloch, L., and Rothstein, A., 1978, N-(4-azido-2-nitrophenyl)-2-aminoethylsulfonate (NAP-taurine) as a photoaffinity probe for identifying membrane components containing the modifier site of the human red blood cell anion exchange system, J. Gen. Physiol. 72:631–649.
Knauf, P.A., Law, F-Y., Tarshis, T., and Furuya, W., 1984, Effects of the transport site conformation on the binding of external NAP-taurine to the human erythrocyte exchange system, J. Gen. Physiol. 83:683–701.
Knauf, P.A., Mann, N.A., Kalwas, J.E., Spinelli, L.J., and Ramjeesingh, M., 1987, Interactions of NIP-taurine, NAP-taurine, and Cl− with the human erythrocyte anion exhange system, Am. J. Physiol. 253:C652–C661.
Knauf, P.A., Mann, N.A., Kalwas, J.E., Spinelli, L.J., and Ramjeesingh, M., 1987, Interactions of NIP-taurine, NAP-taurine, and Cl− with the human erythrocyte anion exhange system, Cell Physiol. 22:C652–C661.
Leaback, D.H., and Walker, P.G., 1961, Studies on glucosaminidase IV. The fluorimetric assay of N-acetyl-β-D-glucosaminidase, Biochem. J. 78:151–156.
Lowry, O.H., and Passonneau, J.V., 1972, A flexible system of enzymatic analysis, Academic Press, New York.
Pillion, D.J., Jeske, A.H., and Leibach, F.H., 1976, Gamma-glutamyl transpeptidase in the urine from an isolated rabbit kidney perfused with and without DMSO, Biochem. Pharmacol. 25:913–918.
Post, R.L., and Sen, A.K., 1967, Sodium and potassium-stimulated ATPase, energy-linked reactions, Methods Enzymol. X:762–768.
Stokols, M.F., Koschier, F.J., Goldinger, J.M., and Hong, S.K., 1981, Renal transport of NAP-taurine, Am. J. Physiol. 241 (Renal Fluid Electrolyte Physiol. 10):F9–F13.
Tisdale, H.D., 1967, Preparation and properties of succinic cytochrome c reductase (Complex II–III), Methods Enzymol. X:213–217.
Zelikovic, I., Stejskal-Lorenz, E., Lohstroh, P., Budreau, A., and Chesney, R.W., 1989, Anion dependence of taurine transport by rat renal brush border membrane vesicles, Am. J. Physiol. 256:F646–F655.
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Chesney, R.W., Budreau, A.M. (1994). Inhibitors of Anion Exchanger Activity Reduce Sodium Chloride-Dependent Taurine Transport by Brush Border Vesicles. In: Huxtable, R.J., Michalk, D. (eds) Taurine in Health and Disease. Advances in Experimental Medicine and Biology, vol 359. Springer, Boston, MA. https://doi.org/10.1007/978-1-4899-1471-2_12
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DOI: https://doi.org/10.1007/978-1-4899-1471-2_12
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