Abstract
A major requirement for the success of BNCT is the selective delivery of boron to individual tumor cells 1,2. Monoclonal antibodies (MoAbs) have been used as selective delivery agents for drugs, toxins and radionuclides to tumor cells for cancer therapy3. However, one major limitation of boron containing immunoconjugates is that their tumor localizing properties may be significantly reduced when compared to native, unmodified antibodies4,5. Primarily for this reason we have been interested in the possibility of using bispecific antibodies (BsAbs) for tumor targeting. BsAbs have two distinct antigen combining sites, one of which can bind to a tumor associated antigen on the cell surface, and the other to a tumoricidal agent6. Since BsAbs have not been chemically modified, they presumably have both immunoreactivity and distribution profiles that are similar to native MoAbs. BsAbs can be produced by fusing two different hybridoma cell lines to produce a hybrid-hybridoma (quadroma). In the present report, we describe the production of quadro-mas secreting BsAbs reactive with polyhedral borane anions and a tumor associated cell surface proteoglycan expressed on glioblastomas and melanomas7.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
Similar content being viewed by others
References
R.F.Barth, A.H.Soloway, and R.G.Fairchild, Boron neutron capture therapy of cancer. Cancer:Res. 50: 1061–1070, 1990.
M.F.Hawthorne, The role of chemistry in the development of boron neutron capture therapy of cancer. Angew.Chem.Int.Ed.Engl, 32: 950–984, 1993.
T.A.Waldmann, Monoclonal antibodies in diagnosis and therapy. Science., 252: 1657–1662, 1991.
R.F.Barth, N.Mafune, F.Alam, D.M.Adams, A.H.Soloway, G.E.Makroglou, et al, Conjugation, purification and characterization of boronated monoclonal antibodies for use in neutron capture therapy. Strahlenther und Onkol., 165: 142–145, 1989.
R.F.Barth, D.M.Adams, A.H.Soloway, F.Alam, and M.V.Darby, Boronated starburst dendrimer-monoclonal antibody immunoconjugates: evaluation as a potential delivery system for neutron capture therapy. Bioconjugate Chem., 5: 58–66, 1994.
O.Nolan and R.O’Kennedy, Bifunctional antibodies: concept, production and applications. Biochemica et Biophysica Acta, 1040: 1–11, 1990.
A.C.Morgan, D.R.Galloway, and R.A.Reisfeld, Production and characterization of monoclonal antibody to a melanoma specific glycoprotein. Hybridoma, 1:27–36, 1981.
R.F.Barth, D.M.Adams, A.H.Soloway, E.B.Mechetner, F.Alam, and A.K.M.Anisuzzaman, Determination of boron in tissues and cells using direct current plasma atomic emisson spectroscopy. Anal.Chem., 63: 890–892, 1991.
O.A.Oredipe, R.F.Barth, J.H.Rotaru, and Z.Steplewski, Modulation of monoclonal antibody affinity and antigenic receptor site expression on human colorectal cancer cells. Antibody, lmmunoconiugates, and Radiopharmaceuticals, 5: 295–306, 1992.
M.Schrappe, G.F.Klier, R.C.Spiro, T.A.Waltz, R.A.Reisfeld, and C.L.Gladson, Correlation ofchondroitin sulfate proteoglycan expression on proliferating brain capillary endothelial cells with the malignant phentype of astroglical cells. Cancer Res. 51: 4986–93, 1991.
L. Liu, R.F. Barth, D.M. Adams, A.H. Soloway, and R. Reisfeld. Bispecific antibodies as targetins agents for boron neutron capture therapy of brain tumors. J. Hematother. 4: 447–483, 1995.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1996 Springer Science+Business Media New York
About this chapter
Cite this chapter
Liu, L., Barth, R.F., Adams, D.M., Soloway, A.H., Reisfeld, R.A. (1996). Potential Use of Bispecific Antibodies (BSABS) for Targeting Gliomas and Melanomas. In: Mishima, Y. (eds) Cancer Neutron Capture Therapy. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-9567-7_7
Download citation
DOI: https://doi.org/10.1007/978-1-4757-9567-7_7
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4757-9569-1
Online ISBN: 978-1-4757-9567-7
eBook Packages: Springer Book Archive