Abstract
Each biological membrane operates differentially on the two compartments it separates and is thus anisotropic in its function. It is reasonable to suppose that the molecular constituents of the two surfaces differ and that this asymmetry constitutes a principal feature of membrane organization. Since membranes are only a few macromolecules thick, it is clear that enumeration of the components at each surface would do much to define the structure as a whole.
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Abbreviations
- RO:
-
right-side-out
- IO:
-
inside-out
- PBS:
-
150 mM NaCl-5 mM Na phosphate (pH 8.0)
- 5P8:
-
5 mM Na phosphate (pH 8.0)
- 0.5P8:
-
0.5 mM Na phosphate (pH 8.0)
- AChase:
-
acetylcholinesterase (acetylcholine hydrolase, E.C. 3.1.1.7)
- G3PD:
-
glyceraldehyde-3-phosphate dehydrogenase (d-glyceraldehyde-3-phosphate
- NAD+ :
-
oxidoreductase [phosphorylating], E.G. 1.2.1.12)
- NADH diaphorase:
-
reduced-NAD: (acceptor) oxidoreductase (E.C. 1.6.99.3)
- DTNB:
-
5,5′-dithiobis-(2-nitrobenzoic acid).
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Steck, T.L. (1974). Preparation of Impermeable Inside-Out and Right-Side-Out Vesicles from Erythrocyte Membranes. In: Korn, E.D. (eds) Methods in Membrane Biology. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-8960-0_4
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DOI: https://doi.org/10.1007/978-1-4615-8960-0_4
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