Abstract
The previous chapters describe methods for recording ionic currents from very small, localized patches of membrane containing only one or at most a few channels. Many such single-channel records have to be averaged to obtain ensemble kinetics or current densities through the membrane. An alternative would be to record from many channels simultaneously, using patch pipettes for a whole-cell recording (Hamill et al., 1981; Chapter 7) or the classic voltage-clamp techniques, with loss of the positional information. Also, the “gigaseals” needed for single-channel recording require extensive proteolytic enzymatic treatment on many cells in order to clean the membrane surface.
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© 1983 Plenum Press, New York
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Stühmer, W., Roberts, W.M., Almers, W. (1983). The Loose Patch Clamp. In: Sakmann, B., Neher, E. (eds) Single-Channel Recording. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-7858-1_8
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DOI: https://doi.org/10.1007/978-1-4615-7858-1_8
Publisher Name: Springer, Boston, MA
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