Abstract
The patch clamp technique revolutionized the study of ion channels and is considered the gold standard of measuring ion channel activity, from the academic laboratory to industrial-scale drug screening. This technique enables the study of ion channels, from single molecules up to the whole-cell ion channel population, and in their native environment. Whilst the study of single protein molecular behavior is the ultimate goal of biophysicists from all fields, this is a routine ability for ion channel specialists. This chapter is aimed at helping the beginner to design patch clamp experiments and to obtain the fundamental micropipette configurations with mammalian cells: cell-attached patch, whole cell, inside-out patch, and outside-out patch.
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References
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Lippiat, J.D., Wrighton, D.C. (2013). Conventional Micropipette-Based Patch Clamp Techniques. In: Gamper, N. (eds) Ion Channels. Methods in Molecular Biology, vol 998. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-351-0_7
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DOI: https://doi.org/10.1007/978-1-62703-351-0_7
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Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-350-3
Online ISBN: 978-1-62703-351-0
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