Abstract
An assembly of a cell wall-like structure on the outmost surface of liposomes has been constructed, which makes liposomes tough against chemical and physicochemical lyses of liposomal membranes caused by external stimuli. In accordance with this idea, we have prepared partly modified polysaccharides, (0-palmitoylpullulan (OPP) and (0-palmitoylamylopectin (OPA), and coated the outmost surface of egg phosphatidylcholine liposomes by them. The efficiency in coating liposomes with the artificial cell wall has been ascertained by four different methods: (1) isolation of polysaccharide-coated liposomes by gel-filtration, (2) reduced permeability for a water soluble material, carboxyfluorescein, encapsulated in the interior of liposomes, (3) increased resistance against the enzymatic lysis with phospholipase D for the coated liposomes, and (4) decreased probability in the enzymatic digestion with pullulanase of the polysaccharide strongly bound to the surface of liposomes. These results suggest a wider usage of the polysaccharide-coated liposomes as an improved drug carrier.
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© 1983 Plenum Press, New York
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Sunamoto, J., Iwamoto, K., Takada, M., Yuzuriha, T., Katayama, K. (1983). Improved Drug Delivery to Target Specific Organs Using Liposomes as Coated with Polysaccharides. In: Chiellini, E., Giusti, P. (eds) Polymers in Medicine. Polymer Science and Technology. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-7643-3_10
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DOI: https://doi.org/10.1007/978-1-4615-7643-3_10
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