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Microbiology of Bivalve Molluscan Shellfish

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Microbiology of Marine Food Products

Abstract

The majority of all seafood-related illnesses in the United States are associated with the consumption of bivalve molluscan shellfish (Anon. 1988). This disproportionate incidence of disease is related to the biology of the animals, the quality of the waters in which they grow, the handling techniques after harvest, and the fact that they are frequently consumed raw. This chapter will consider all these factors from a microbiological standpoint.

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References

  • Abeyta, C, Kaysner, C, Wekell, M., Sullivan, J., and Stelma, G. 1986. Recovery of Aeromonas hydrophila from oysters implicated in an outbreak of foodborne illness. J. Food Protect. 49:643–646.

    Google Scholar 

  • American Public Health Association. 1970. Recommended Procedures for the Examination of Sea Water and Shellfish, fourth edition. American Public Health Association, New York.

    Google Scholar 

  • Andrews, W., Diggs, C, Presnell, M., Miescier, J., Wilson, C, Goodwin, C, Adams, W., Furfari, S., and Musselman, J. 1975. Comparative validity of members of the total coliform and fecal coliform groups for indicating the presence of Salmonella in the Eastern oyster, Crassostrea virginica. J. Milk Food Technol. 38:453–456.

    Google Scholar 

  • Anonyomous. 1986. Code of Federal Regulations 21:161. 130.

    Google Scholar 

  • Anonyomous. 1988. Seafood Safety: Seriousness of Problems and Efforts to Protect Consumers. GAO/RCED-88-135, United States General Accounting Office, Washington, D.C.

    Google Scholar 

  • Bernard, F. R. 1970. Occurrence of the spirochaete genus Cristispira in western Canadian marine bivalves. Veliger 13:33–36.

    Google Scholar 

  • Buck, J. D., Bubucis, P. M., and Combs, T. J. 1977. Occurrence of human-associated yeasts in bivalve shellfish from Long Island Sound. Appl. Environ. Microbiol. 33:370–378.

    PubMed  CAS  Google Scholar 

  • Burrell, V. G. 1974. Thermal bleaching of red algal pigment in shucked oysters. Marine Fish. Rev. 36:19–22.

    Google Scholar 

  • Chai, T., Pace, J., and Cossaboom, T. 1984. Extension of shelf-life of oysters by pasteurization in flexible pouches. J. Food Sci. 49:331–333.

    Article  Google Scholar 

  • Colwell, R. R. and Liston, J. 1960. Microbiology of shellfish: bacteriological study of the natural flora of Pacific oysters (Crassostrea gigas). Appl. Microbiol. 8: 104–109.

    PubMed  CAS  Google Scholar 

  • Connors, T. J., and Steinberg, M. A. 1964. Preservation of fresh, unfrozen fishery products by low-level radiation. II. Organoleptic studies on radiation pasteurized soft shell clam meats. Food Technol. 18:113–116.

    Google Scholar 

  • Cook, D. W. 1984. Fate of enteric bacteria in estuarine sediments and oyster feces. J. Miss. Acad. Sci. 29:71–76.

    Google Scholar 

  • Cook, D. W., and Eilender, R. D. 1986. Relaying to decrease the concentration of oyster-associated pathogens. J. Food Protect. 49:196–202.

    Google Scholar 

  • Cook, D. W., and Ruple, A. D. 1989. Indicator bacteria and Vibrionaceae multiplication in post-harvest shellstock oysters. J. Food Protect. 52:343–349.

    Google Scholar 

  • Covert, D., and Woodburn, M. 1972. Relationships of temperature and sodium chloride concentration to the survival of Vibrio parahaemolyticus in broth and fish homogenate. Appl. Microbiol. 23:321–325.

    PubMed  CAS  Google Scholar 

  • Cox, J. R. 1965. Bacteriological studies on the shelf life of soft clams (Mya arenaria). J. Milk Food Technol. 28:32–35.

    Google Scholar 

  • DiGirolamo, R., Liston, J., and Matches, J. 1970a. The effects of freezing on the survival of Salmonella and E. coli in Pacific oysters. J. Food Sci. 35:13–16.

    Article  Google Scholar 

  • DiGirolamo, R, Liston, J., and Matches, J. R. 1970b. Survival of virus in chilled, frozen, and processed oysters. Appl. Microbiol. 20:58–63.

    PubMed  CAS  Google Scholar 

  • Dimitroff, V. T. 1926. Spirochaetes in Baltimore market oysters. J. Bacteriol. 12:135–177.

    PubMed  CAS  Google Scholar 

  • Eilender, R., Cook, D., Sheladia, V., and Johnson, R. 1980a. Enterovirus and bacterial evaluation of Mississippi oysters. Gulf Res. Rep. 6:371–376.

    Google Scholar 

  • Eilender, R., Mapp, J., Middlebrooks, B., Cook, D., and Cake, E. 1980b. Natural enterovirus and fecal coliform contamination of Gulf Coast oysters. J. Food Protect. 43:105–110.

    Google Scholar 

  • Fieger, E., and Novak, A. 1961. Microbiology of Shellfish Deterioration. In: Georg Borgstrom (ed.). Fish as Food, Vol. 1. Academic Press, New York.

    Google Scholar 

  • Fleet, G. H. 1978. Oyster depuration-a review. Food Technol. Aust. 30:444–454.

    CAS  Google Scholar 

  • Food and Drug Administration. 1983. Bacteriological quality of approved area summer harvested Louisiana oysters during harvest and interstate shipment. Food and Drug Administration, Shellfish Sanitation Branch, Northeast Technical Services Unit, North Kingston, R. I.

    Google Scholar 

  • Food and Drug Administration. 1989a. National Shellfish Sanitation Program Manual of Operations, Part I, Sanitation of Shellfish Growing Areas. Public Health Service, Shellfish Sanitation Branch, Washington, D.C.

    Google Scholar 

  • Food and Drug Administration. 1989b. National Shellfish Sanitation Program Manual of Operations, Part II, Sanitation of the Harvesting, Processing and Distribution of Shellfish. Public Health Service, Shellfish Sanitation Branch, Washington, D.C.

    Google Scholar 

  • Fraiser, M. B., and Koberger, J. A. 1984. Incidence of salmonellae in clams, oysters, crabs and mullet. J. Food Protect. 47:343–345.

    Google Scholar 

  • Fugate, K. J., Cliver, D. O., and Hatch, M. T. 1975. Enteroviruses and potential bacterial indicators in Gulf Coast oysters. J. Milk Food Technol. 38:100–104.

    Google Scholar 

  • Galtsoff, P. S. 1964. The American Oyster. Fishery Bulletin No. 64. U.S. Department of the Interior, U.S. Government Printing Office, Washington, D.C.

    Google Scholar 

  • Gardner, E. A., and Watts, B. M. 1956. Correlation of pH and quality of shucked southern oysters. Commer. Fish. Rev. 18:8–15.

    CAS  Google Scholar 

  • Garland, C. D., Nash, G. V., and McMeekin, T. A. 1982. Absence of surface-associated microorganisms in adult oysters (Crassostrea gigas). Appl. Environ. Microbiol. 44:1205–1211.

    PubMed  CAS  Google Scholar 

  • Goatcher, L. J., and Westhoff, D. C. 1975. Repression of Vibrio parahaemolyticus by Pseudomonas species isolated from processed oysters. J. Food Sci. 40:533–536.

    Article  Google Scholar 

  • Goatcher, L. J., Engler, S. E., Wagner, D. C, and Westhoff, D. C. 1974. Effect of storage at 5°C on survival of Vibrio parahaemolyticus in processed Maryland oysters (Crassostrea virginica). J. Milk Food Technol. 37:74–77.

    Google Scholar 

  • Goyal, S. M., Gerba, C. P., and Melnick, J. L. 1979. Human enteroviruses in oysters and their overlying waters. Appl. Environ. Microbiol. 37:572–581.

    PubMed  CAS  Google Scholar 

  • Greenberg, A. E., and Hunt, D. A. (eds.). 1985. Laboratory Procedures for the Examination of Seawater and Shellfish, fifth edition. American Public Health Association, Washington, DC.

    Google Scholar 

  • Hackney, C, Rippen, T., and Sanders, L. 1988. Quality of previously frozen oysters repacked for the fresh market. In: Proceedings Twelfth Annual Conference of the Tropical and Subtropical Fisheries Technological Society of the Americas, Florida Sea Grant College Program, SGR-92, Gainesville, Florida, pp. 421–428.

    Google Scholar 

  • Heen, E., and Karsti, O. 1965. Fish and Shellfish Freezing. In: G. Borgstrom (ed.). Fish as Food, vol. IV, Processing: Part 2. Academic Press, New York.

    Google Scholar 

  • Hoff, J. C, Beck, W. J., Ericksen, T.H., Vasconcelos, G. J., and Presnell, M. W. 1967a. Time-temperature effects on the bacteriological quality of stored shellfish. I. Bacteriological changes in live shellfish: Pacific oysters (Crassostrea gigas), Olympia oysters (Ostrea lurida), Native Littleneck clams (Protothaca staminea), and Manila clams (Venerupis japonica). J. Food Sci. 32:121–124.

    Article  Google Scholar 

  • Hoff, J. C, Beck, W. J., Ericksen, T. H., Vasconcelos, G. J., and Presnell, M. W. 1967b. Time-temperature effects on the bacteriological quality of stored shellfish. II. Bacteriological changes in shucked Pacific oysters (Crassostrea gigas) and Olympia oysters (Ostrea lurida). J. Food Sci. 32:125–129.

    Article  Google Scholar 

  • Hood, M. A. 1983. Effects of harvesting waters and storage conditions on yeast populations in shellfish. J. Food Protect. 46:105–108.

    Google Scholar 

  • Hood, M. A., Ness, G. E., Rodrick, G. E., and Blake, N. J. 1983. Effects of storage on microbial loads of two commercially important shellfish species, Crassostrea virginica and Mercenaria campechiensis. Appl. Environ. Microbiol. 45: 1221–1228.

    PubMed  CAS  Google Scholar 

  • Hood, M. A., Baker, R. M., and Singelton, F. L. 1984. Effect of processing and storing oyster meats on concentrations of indicator bacteria, vibrios and Aeromonas hydrophi1a. J. Food Protect. 47:598–601.

    Google Scholar 

  • Hunter, A. C. 1920. A pink yeast causing spoilage in oysters. U.S. Department of Agriculture Bulletin No. 819, p. 24.

    Google Scholar 

  • Hunter, A. C, and Harrison, C. W. 1928. Bacteriology and chemistry of oysters, with special reference to regulatory control of production, handling and shipment. US Dept. Agric. Tech. Bull. 64:1–75.

    CAS  Google Scholar 

  • Hunter, A. C, Linden, B. A. 1923. An investigation of oyster spoilage. Am. Food J. 18:538–540.

    CAS  Google Scholar 

  • Jarvis, N. D. 1943. Principles and methods in the canning of fishery products. US Fish Wildlf. Serv. Res. Rep. No. 7, 366 pp.

    Google Scholar 

  • Jay, J.M. 1986.Modern Food Microbiology, third edition. Van Nostrand Reinhold, New York.

    Google Scholar 

  • Johnson, H. C, and Liston, J. 1973. Sensitivity of Vibrio parahaemolyticus to cold in oysters, fish fillets and crabmeat. J. Food Sci. 38:437–441.

    Article  Google Scholar 

  • Johnson, W. G., Salinger, A. C, and King, W. C. 1973. Survival of Vibrio parahaemolyticus in oyster shellstock at two different storage temperatures. Appl. Microbiol. 26:122.

    PubMed  Google Scholar 

  • Kelly, C. B. 1964. Time-temperature effect on bacteriological quality of stored oysters. In: Proceedings, Fifth National Shellfish Sanitation Workshop, pp. 193–202.

    Google Scholar 

  • Koburger, J. A., and Miller, M. L. 1984. Plesiomonas shigelloides: a new problem for the oyster industry? In: Proceedings of the Ninth Annual Tropical and Subtropical Fisheries Conference of the Americas. TAMU-SG-85-106, pp. 337–342.

    Google Scholar 

  • Kueh, G., and Chan, K. 1985. Bacteria in bivalve shellfish with special reference to the oyster. J. Appl. Bacteriol. 59:41–47.

    Article  PubMed  CAS  Google Scholar 

  • Liuzzo, J. A., and Novak, A. F. 1975. Correlation of organoleptic evaluation with chemical and microbiological tests in oysters. Food Prod. Dev. 9:78.

    Google Scholar 

  • Liuzzo, J. A., Barone, W. B., and Novak, A. F. 1966. Stability of B-vitamins in Gulf oysters preserved by gamma radiation. Fed. Proc. 25:722.

    Google Scholar 

  • Liuzzo, J. A., Lagarde, S. C., Grodner, R. M., and Novak, A. F. 1975. A total reducing substance test for ascertaining oyster quality. J. Food Sci. 40:125–128.

    Article  CAS  Google Scholar 

  • Lovelace, T. E., Tubiash, H., and Colwell, R. R. 1968. Quantitative and qualitative commensal bacterial flora of Crassostrea virginica in Chesapeake Bay. In: Proceedings of the National Shellfisheries Association. 58:82–87.

    Google Scholar 

  • McCormack, G. 1950. Pink yeast isolated from oysters grown at temperatures below freezing. Commer. Fish. Rev. 12:28.

    Google Scholar 

  • McCormack, G. 1956. Growth characteristics of the pink yeast that causes discoloration of oysters. Commer. Fish. Rev. 18:21–22.

    Google Scholar 

  • McKee, L. G. 1963. The oyster, clam, scallop and abalone fisheries. In: Stansby, M. E. (ed.). Industrial Fishery Technology. Van Nostrand Reinhold, New York, pp. 183–192.

    Google Scholar 

  • Motes, M. L. 1982. Effect of chlorinated wash water on Vibrio cholerae in oyster meats. J. Food Sci. 47:1028–1029.

    Article  Google Scholar 

  • Murchelano, R. A., and Brown, C. 1968. Bacteriological study of the natural flora of the Eastern oyster, Crassostrea virginica. J. Invert. Pathol. 11:519–520.

    Article  Google Scholar 

  • Novak, A. F., Liuzzo, J. A., Grodner, R. M., and Lovell, R. T. 1966. Radiation pasteurization of Gulf Coast oysters. Food Technol. 20:103–104.

    Google Scholar 

  • Oliver, J. D. 1981. Lethal cold stress of Vibrio vulnificus in oysters. Appl. Environ. Microbiol. 41:710–717.

    PubMed  CAS  Google Scholar 

  • Pace, J., Wu, G. Y., and Chai, T. 1988. Bacterial flora in pasteurized oysters after refrigerated storage. J. Food Sci. 53:325–327.

    Article  Google Scholar 

  • Paille, D., Hackney, C, Reily, L., Cole, M., and Kilgen, M. 1987. Seasonal variation in the fecal coliform population of Louisiana oysters and its relationship to microbiological quality. J. Food Protect. 50:545–549.

    Google Scholar 

  • Peixotto, S. C. S., Finne, G., Hanna, M. O. and Vanderzant, C. 1979. Presence, growth and survival of Yersinia enterocolitica in oysters, shrimp and crab. J. Food Protect. 42:974–981.

    Google Scholar 

  • Presnell, M. W., and Kelly, C. B. 1961. Bacteriological studies of commercial shellfish operations on the Gulf Coast. U.S. Public Health Service Technical Report F-61-9.

    Google Scholar 

  • Reily, L. A., and Hackney, C. R. 1985. Survival of Vibrio cholerae during cold storage in artificially contaminated seafoods. J. Food Sci. 50:838–839.

    Article  Google Scholar 

  • Richards, G. P. 1988. Microbial purification of shellfish: a review of depuration and relaying. J. Food Protect. 51:218–251.

    Google Scholar 

  • Rippey, S. R., and Verber, T. L. 1986. Shellfish Borne Disease Outbreaks. Food and Drug Administration, Shellfish Sanitation Branch, Northeast Technical Services Unit, Davisville, Rhode Island.

    Google Scholar 

  • Ronk, R. J. 1988. Emerging trends: Vibrio vulnificus. J. Assoc. Food Drug Offic. 52:7–11.

    Google Scholar 

  • Rosen, B. 1966. Accumulation of organic acids during cold storage of shucked soft clams, (Mya arenaria) (L.), in relation to quality. Fish. Indust. Res. 3:5–11.

    CAS  Google Scholar 

  • Rowse, A. J., and Fleet, G. H. 1982. Viability and release of Salmonella charity and Escherichia coli from oyster feces. Appl. Environ. Microbiol. 44:544–548.

    PubMed  CAS  Google Scholar 

  • Shiflett, M. A., Lee, J. S. and Sinnhuber, R. O. 1966. Microbial flora of irradiated dungeness crabmeat and Pacific oysters. Appl. Microbiol. 14:411–415.

    PubMed  CAS  Google Scholar 

  • Sidwell, V. D., Loomis, A. L., and Grodner, R. M. 1979. Geographic and monthly variation in composition of oysters, Crassostrea virginica. Marine Fish. Rev. 41:13–17.

    Google Scholar 

  • Sieling, F. W. 1971. Harmless coloration of oysters explained. Commer. Fish. News 4:1–2.

    Google Scholar 

  • Singleton, P., and Sainsbury, D. 1978. Dictionary of Microbiology. John Wiley & Sons, New York.

    Google Scholar 

  • Sobsey, M., Hackney, C, Carrick, R., Ray, B., and Speck, M. 1980. Occurrence of enteric bacteria and viruses in oysters. J. Food Protect. 43:111–113.

    Google Scholar 

  • Son, N. T., and Fleet, G. H. 1980. Behavior of pathogenic bacteria in the oyster, Crassostrea commercialism during depuration, re-laying, and storage. Appl. Environ. Microbiol. 40:994–1002.

    PubMed  CAS  Google Scholar 

  • Tall, B. D., and Nauman, R. K. 1981. Scanning electron microscopy of Cristispira species in Chesapeake Bay oysters. Appl. Environ. Microbiol. 42:336–343.

    PubMed  CAS  Google Scholar 

  • Tanikawa, E., and Doha, S. 1965. Heat processing of shellfish. In: G. Borgstrom (ed.). Fish as Food, Vol. IV, Processing: Part 2. Academic Press, New York.

    Google Scholar 

  • Thompson, C. A., Vanderzant, C, and Ray, S. M. 1976. Effect of processing, distribution and storage on Vibrio parahaemolyticus and bacterial counts of oysters (Crassostrea virginica). J. Food Sci. 41:123–127.

    Article  Google Scholar 

  • Tierney, J. T., Sullivan, R., Peeler, J. T., and Larkin, E. P. 1982. Persistence of polioviroses in shellstock and shucked oysters stored at refrigeration temperatures. J. Food Protect. 45:1135–1137.

    Google Scholar 

  • Vanderzant, C, Thompson, C. A., Jr., and Ray, S. M. 1973. Microbial flora and level of Vibrio parahaemolyticus of oysters (Crassostrea virginica), water and sediment from Galveston Bay. J. Milk Food Technol. 36:447–452.

    Google Scholar 

  • Vasconcelos, G. J., and Lee, J. S. 1972. Microbial flora of Pacific oysters (Crassostrea gigas) subjected to ultraviolet-irradiated seawater. Appl. Microbiol. 23:11–16.

    PubMed  CAS  Google Scholar 

  • Vasconcelos, G. J., Jakubowski, W., and Ericksen, T. H. 1969. Bacteriological changes in shellfish maintained in an estuarine environment. In: Proceedings of the National Shellfisheries Association 59:67–83.

    Google Scholar 

  • Vaughn, J. M., Landry, E. F., Vicale, T. J., and Dahl, M. D. 1980. Isolation of naturally occurring enteroviruses from a variety of shellfish species residing in Long Island and New Jersey marine embayments. J. Food Protect. 43:95–98.

    Google Scholar 

  • Wait, D. A., Hackney, C. R., Carrick, R. J., Lovelack, G., and Sobsey, M. D. 1983. Enteric bacteria and viral pathogens and indicator bacteria in hard shell clams. J. Food Protect. 46:493–496.

    Google Scholar 

  • Wilson, T. E., and McCleskey, C. S. 1951. The effect of storage on indicator organisms in shucked oysters and a comparison of methods for the determination of enterococci. Food Res. 16:377–382.

    Article  Google Scholar 

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Cook, D.W. (1991). Microbiology of Bivalve Molluscan Shellfish. In: Ward, D.R., Hackney, C. (eds) Microbiology of Marine Food Products. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-3926-1_2

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