Abstract
Microencapsulation is the envelopment of small solid particles, liquids, or gas bubbles with coating. Many methods and materials for preparing microcapsules have been developed and employed in cosmetics, pharmaceutics, and agricultural products. A core material is encapsulated through phase separation of polymer solutions, in situ interfacial polymerization, spray drying, or other techniques. Since most of the techniques used include processes in which the core material is exposed to organic solvents, high temperature, or chemical reactions, they are not applicable to the microencapsulation of mammalian cells, which are so fragile that they are easily destroyed when exposed to such radical processes. Few methods that can successfully microencapsulate mammalian cells without damaging them have been reported. Polysaccharides, such as alginic acid (Lim and Sun 1980), agarose (Iwata et al 1989) and chitosan (Matthew et al 1993), have been used for microencapsulation of mammalian cells, because insoluble membranes or firm hydrogels can be formed from aqueous solutions of these polysaccharides by bringing the solutions into contact with polyvalent cationic metal ions or polyelectrolytes, and by lowering the temperature of the solutions.
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Iwata, H., Ikada, Y. (1999). Agarose. In: Kühtreiber, W.M., Lanza, R.P., Chick, W.L. (eds) Cell Encapsulation Technology and Therapeutics. Birkhäuser, Boston, MA. https://doi.org/10.1007/978-1-4612-1586-8_9
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DOI: https://doi.org/10.1007/978-1-4612-1586-8_9
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