Abstract
Physiological and morphological changes upon infection by viruses have been observed in many cell lines used for the production of proteins and viruses. Here we demonstrate the utility of single-cell analysis to monitor various cellular parameters in insect cells-baculovirus and HEK293-adenovirus systems, including DNA content, cell size and cell granularity and their correlation with the propagation of virus and production of recombinant protein. Analysis of infected cells at the single-cell level by flow cytometry was shown to be an effective method to monitor the changes in virus titre and intracellular protein. The results show that flow cytometry is an effective tool for process monitoring of virus-cell systems that allows the design of optimum harvest strategies.
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© 2007 Springer
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Sandhu, K., Al-Rubeai, M. (2007). Flow Cytometry as a Tool for Process Monitoring of Virus-cell Systems. In: Smith, R. (eds) Cell Technology for Cell Products., vol 3. Springer, Dordrecht. https://doi.org/10.1007/978-1-4020-5476-1_99
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DOI: https://doi.org/10.1007/978-1-4020-5476-1_99
Publisher Name: Springer, Dordrecht
Print ISBN: 978-1-4020-5475-4
Online ISBN: 978-1-4020-5476-1
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