Abstract
A serum-free process for influenza virus vaccine production (equine and human) in roller bottles and microcarrier systems in 5L-stirred tank and 2Lwave bioreactor (Cytodex 1) is described. MDCK cells were adapted from growth in serum containing GMEM medium to serum-free Ex-Cell MDCK medium. Virus titers of 2.0–2.9 log HA units/ 100 μL were obtained. Omission of the medium exchange before infection has clearly simplified the process.
Access provided by Autonomous University of Puebla. Download to read the full chapter text
Chapter PDF
Similar content being viewed by others
Keywords
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
References
Merten, O.W.; 2002, Dev Biol Stand, 111: 233–257.
Genzel, Y., Behrendt, I., König, S., Sann, H., Reichl, U.; 2004, Vaccine, 22 (17–18): 2202–2208.
Genzel, Y., Ritter, J.B., König, S., Alt, R., Reichl, U.; 2005, Biotechnol. Progr., 21 (1): 58–69.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2007 Springer
About this paper
Cite this paper
Genzel, Y. et al. (2007). Serum-free Influenza Vaccine Production with MDCK Cells in Wave-bioreactor and 5L-stirred Tank Bioreactor. In: Smith, R. (eds) Cell Technology for Cell Products., vol 3. Springer, Dordrecht. https://doi.org/10.1007/978-1-4020-5476-1_97
Download citation
DOI: https://doi.org/10.1007/978-1-4020-5476-1_97
Publisher Name: Springer, Dordrecht
Print ISBN: 978-1-4020-5475-4
Online ISBN: 978-1-4020-5476-1
eBook Packages: Chemistry and Materials ScienceChemistry and Material Science (R0)