Abstract
The use of a perfusion system prevented unspecific inhibitions effects on cell growth for batch cultivations of adherent MDCK cells used for an equine influenza A vaccine production process. It also increased viable cell numbers. An enzyme sensor for monitoring and closed loop control of metabolites operated stable for at least 55 hours.
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Genzel, Y., Behrendt, I., König, S., Sann, H., Reichl, U., 2002, Metabolism of MDCK cells during growth and Influenza virus production in large-scale microcarrrier culture; Vaccine 22(17–18): 2202–2208.
Glacken, M. W., Fleischaker, R. J., Sinskey, A. J., 1985, Reduction of waste product excretion via nutrient control: possible strategies for maximizing product and cell yields on serum in cultures of mammalian cells, Biotech. Bioeng. 28: 1376–1389.
Hauser, H., Wagner, R., 1997, Mammalian cell biotechnology in protein production, W. de Gruyther.
Möhler, L., Flockerzi, D., Sann H., Reichl, U., 2005, A mathematical model of Influenza A virus production in large-scale microcarrier culture; Biotech. Bioeng. 90 (1): 46–58.
Sann, H., Bock, A., Reichl, U., 2002, Device and method for extracting liquid samples, WO 2004/ 033077 A2.
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Bock, A., Reichl, U. (2007). Closed Loop Control of Perfusion Systems in High-density Cell Culture. In: Smith, R. (eds) Cell Technology for Cell Products., vol 3. Springer, Dordrecht. https://doi.org/10.1007/978-1-4020-5476-1_94
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DOI: https://doi.org/10.1007/978-1-4020-5476-1_94
Publisher Name: Springer, Dordrecht
Print ISBN: 978-1-4020-5475-4
Online ISBN: 978-1-4020-5476-1
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