Abstract
Due to regulatory requirements today’s state-of-the-art cell culture production processes are performed in well defined serum- or protein-free media. Nevertheless, development of recombinant production cell lines is often performed under serum-containing conditions. Accordingly, after clonal selection the cells have to be adapted to serum-free medium, which bears the risk of losing productivity or product quality and significantly extends development time. Through extensive medium screening and testing of multiple transfection methods a CHO dhfr- host cell based procedure was established, allowing the serum-free performance of all steps being essential for the development of a recombinant cell line
Access provided by Autonomous University of Puebla. Download to read the full chapter text
Chapter PDF
Similar content being viewed by others
Keywords
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2007 Springer
About this paper
Cite this paper
Kuchenbecker, M., Rehberger, B., Schriek, M., Gackowski, J., Fehrenbach, R. (2007). Establishment of Recombinant CHO Cell Lines Under Serum-free Conditions. In: Smith, R. (eds) Cell Technology for Cell Products., vol 3. Springer, Dordrecht. https://doi.org/10.1007/978-1-4020-5476-1_8
Download citation
DOI: https://doi.org/10.1007/978-1-4020-5476-1_8
Publisher Name: Springer, Dordrecht
Print ISBN: 978-1-4020-5475-4
Online ISBN: 978-1-4020-5476-1
eBook Packages: Chemistry and Materials ScienceChemistry and Material Science (R0)