Abstract
Recombinant CHO, rCHO, cells are usually cultured at 37 °C. Unlike the specific growth rate, which decreases at low temperature, effects of low temperature culture on specific productivity rate are not so clear. We studied low temperature culture effects on sCR1 productivity in rCHO cells and reported here. The specific growth rate of rCHO increased according to increase of culture temperature. The specific rate of sCR1 at 37 °C was similar to that at 35 °C, however that at 33 °C increased by 1.3 compared with that at 37 °C. These results suggest that the productivity of sCR1 could become higher by using the shift of culture temperature.
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Matsuoka, H., Shimizu, C., Takeda, T. (2007). Low Temperature Culture Effects on sCR1 Productivity by rCHO Cells. In: Smith, R. (eds) Cell Technology for Cell Products., vol 3. Springer, Dordrecht. https://doi.org/10.1007/978-1-4020-5476-1_69
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DOI: https://doi.org/10.1007/978-1-4020-5476-1_69
Publisher Name: Springer, Dordrecht
Print ISBN: 978-1-4020-5475-4
Online ISBN: 978-1-4020-5476-1
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