Abstract
EB14 is a genetically stable diploid cell line derived from chicken embryonic stem cells. EB14 cells demonstrate great potential in virus and recombinant protein production for prophylactic and therapeutic purposes. The present study aims at identifying metabolic needs of the EB14 cells so that specific nutrients can be provided adequately and in a timely manner. EB14 cells exhibit unique metabolic patterns in various serum-containing and serum-free media, and are highly sensitive to nutritional supply and waste accumulation in culture. Typically, EB14 cells require frequent replenishment of culture media to stay viable. The ratio of aerobic to anaerobic metabolism was calculated based on carbon and nitrogen energy source consumption rates in EB14 cells. As a result, optimal media formulations and culture processes were developed to support high viable cell growth supporting recombinant and attenuated viral vaccine production. Chemical and cellular analysis of EB14 cell cultures with specific viable cell density (VCD), culture longevity and viral productivity will be discussed.
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Keywords
- Recombinant Protein Production
- Viable Cell Density
- Chicken Embryonic Fibroblast
- Waste Accumulation
- Cell Culture Platform
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© 2007 Springer
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Hartshorn, J. et al. (2007). Understanding Metabolic Needs of EB14 Cells in Culture. In: Smith, R. (eds) Cell Technology for Cell Products., vol 3. Springer, Dordrecht. https://doi.org/10.1007/978-1-4020-5476-1_101
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DOI: https://doi.org/10.1007/978-1-4020-5476-1_101
Publisher Name: Springer, Dordrecht
Print ISBN: 978-1-4020-5475-4
Online ISBN: 978-1-4020-5476-1
eBook Packages: Chemistry and Materials ScienceChemistry and Material Science (R0)