Cryopreservation of pollen is required for carrying out investigations in both fundamental and applied aspects of pollen biology. Besides the already existing role of pollen cryobanks in breeding, there are many promising applications which have come to focus with the recent advances in allied bio-scientific areas. Crossing desirable genotypes involves multiple and staggered plantings in order to synchronize flowering. This can be avoided when cryopreserved viable pollen is available, facilitating hybrids between genera, species and genotypes. This could effectively conserve field and greenhouse space. The international transfer of germplasm in the form of dry pollen is not generally restricted (Hoekstra 1995). Moreover, this will eliminate the need to grow plant populations to produce pollen. Pollen is usually subjected to less stringent quarantine restrictions. The importance of pollen cryopreservation in horticultural species is well documented (Ganeshan and Rajasekharan 2000a; Kobayashi et al. 1978).
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References
Alexander MP (1987) A method for staining pollen tubes in pistil. Stain Technol 62: 107-112
Alexander MP (1980) A versatile stain for pollen, fungi, yeast and bacteria. Stain Technol 53: 13-18
Alexander MP, Ganeshan S (1989) An improved cellophane method for in vitro germination of recalcitrant pollen. Stain Technol 64: 225-227
Alexander MP, Ganeshan S (1993) Pollen Storage. In: Chadha KL, Pareek OP (ed.) Advances in Horticulture Vol. I. Fruit Crops Part I. Malhotra Publishing House, New Delhi-110 064, India pp 481-486
Anonymous (2006) Establishment of seed and pollen cryobank for ex situ conservation and sustainable utilization of orchids of Western Ghats-(2005) Project Report findings submitted to the Department of Biotechnology, Government of India by William Decruze, GM Nair, S Ganeshan
Barnabas B, Kovacs G (1997) Storage of pollen. In: Shivanna KR, Sawney VK (ed.) Pollen Biotechnology for Crop Production and Improvement. pp 293-314. Cambridge University Press UK
Berthoud J (1997) Strategies for conservation of genetic resources in relation with their utilization. Euphytica 96: 1-12
Brewbaker JL, Kwak BH (1963) The essential role of calcium ion in pollen germination and pollen tube growth. Am J Bot 50: 859-865
Ganeshan S (1985) Cryogenic preservation of grape pollen. Vitis 24: 169-173
Ganeshan S (1986a) Cryogenic preservation of papaya pollen. Sci Hort 28: 65-70
Ganeshan S (1986b) Viability and fertilising capacity of onion pollen (Allium cepa L.) stored in liquid nitrogen. Trop Agric (Trinidad) 63: 46-48
Ganeshan S, Alexander MP (1991) Cryogenic preservation of lemon (Citrus limon Burm.) pollen. Gartenbauwissenschaft 56: 228-230
Ganeshan S, Rajasekharan PE (1995) Genetic conservation through pollen storage in ornamental crops. In: Chadha KL, Bhattacharjee SK (eds.) Advances in Horticulture 1995, Vol.12-Part-1 Ornamental Crops Malhotra Publishing House, New Delhi-110 064, India pp 87-108
Ganeshan S, Rajasekharan PE (2000a) Conservation of nuclear genetic diversity (NGD) in citrus genepool: Implications in citrus breeding and genetic conservation. In: High Tech Citrus Management. Proceedings of International Symposium on Citrus Culture (Nov. 23-27, 1999) held at NRC for Citrus, Nagpur pp 85-90
Ganeshan S, Rajasekharan PE (2000b) Current status of pollen cryopreservation research: Relevance to tropical horticulture. In: Engelmann F, Takagi H (eds.) Cryopreservation of Tropical Plant Germplasm. Current Research Progress and Applications. IPGRI, Rome/JIRCAS, Japan pp 360-365
Ganeshan S, Sulladmath VV (1983) Pollen storage studies on Citrus limon Burm. Varietal differences and influence of flower types. Gartenbauwissenschaft 48: 51-54
Grout BWW, Roberts AV (1995) Storage of free pollen, pollen embryos and the zygotic embryos of seed by cryopreservation and freeze drying. In: Grout B (ed.) Genetic Preservation of Plant Cells In Vitro. Springer Verlag, Berlin pp 63-74
Hoekstra FA (1995) Collecting pollen for genetic resources conservation. In: Guarino L, Rao VR, Reid R (eds.) Collecting Plant Genetic Diversity. Technical Guidelines. CAB International, Wallingford, UK pp 527-550
Hanna WW, Towill LE (1995) Long term pollen storage. In: Janick J (ed.) Plant Breeding Reviews. Wiley New York pp 179-207
Kobayashi S, Ikeda SI, Nakatani M (1978) Long term storage of citrus pollen. In: Akihama T, Nakajima K (eds.) Long Term Preservation of Favourable Germplasm in Arboreal Crops. Ministry of Agriculture and Forestry, Tsukuba-gun Ibaraki ken pp 8-12
Olmo HP (1942) Storage of grape pollen. Proc Am Soc Hort Sci 41: 219-224
Rajasekharan PE, Ganeshan S (1992) Fertilizing ability of cryopreserved mango pollen, 4th International Mango Symposium, FL
Rajasekharan PE, Ganeshan S (1994) Freeze preservation of rose pollen in liquid nitrogen: Feasibility, viability and fertility status after long-term storage. J Hort Sci 69: 565-569
Rajasekharan PE, Ganeshan S (2001-2002) Cryogenic preservation of mango pollen. J Palynol 37-38: 67-71
Rajasekharan PE, Ganeshan S (2003) Feasibility of pollen cryopreservation in Capsicum sp. Capsicum Eggplant Newsl 22: 87-90
Rajasekharan PE, Alexander MP, Ganeshan S (1998) Long term pollen preserva-tion of wild tomato, brinjal species and cultivars in liquid nitrogen. Indian J Pl Gen Resour 11: 117-120
Rajasekharan PE, Ganeshan S, Srinivasan VR (2003) Polbase: Digitalisation of information management system for pollen cryobanks. National Seminar on Bioinformatics and Biodiversity Data Management. TBGRI 15-17 May,2003, Thiruvananthapuram, India. Book of Abstracts pp 10
Rajasekharan PE, Ganeshan S, Thamizharasu V (1995) Expression of trifoliate leaf character in Citrus limonia × Poncirus trifoliata hybrids through cryostored pollen. J Hort Sci 70: 485-490
Rajasekharan PET, Rao M, Janakiram T, Ganeshan S (1994) Freeze preservation of Gladiolus pollen. Euphytica 80: 105-109
Sakai A, Kobayashi S, Oiyama I (1990) Cryopreservation of nucellar cells of navel orange (Citrus sinensis Osb. var. brasiliensis Tanaka) by vitrification. Plant Cell Rep 9: 30-33
Shashikumar S (2006) Pollen Biology of Few Horticulturally Important Plants. PhD Thesis. Bangalore University, Bangalore
Shivanna KR, Rangaswamy NS (1993) Pollen Biology: A Laboratory Manual. Narosa Publishing House, Calcutta, India
Stanley RG, Linskens HF (1974) Pollen: Biology, biochemistry, and management. Springer-Verlag, New York
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Ganeshan, S., Rajasekharan, P.E., Shashikumar, S., Decruze, W. (2008). Cryopreservation of Pollen. In: Reed, B.M. (eds) Plant Cryopreservation: A Practical Guide. Springer, New York, NY. https://doi.org/10.1007/978-0-387-72276-4_17
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