Abstract
Principles of oxygen consumption, oxygen transport, suspension, and mixing are discussed in the context of propagating aggregates of plant tissue in liquid suspension bioreactors. Although micropropagated plants have a relatively low biological oxygen demand (BOD), the relatively large tissue size and localization of BOD in meristematic regions will typically result in oxygen mass transfer limitations in liquid culture. In contrast to the typical focus of bioreactor design on gas-liquid mass transfer, it is shown that media-solid mass transfer limitations limit oxygen available for aerobic plant tissue respiration. Approaches to improve oxygen availability through gas supplementation and bioreactor pressurization are discussed. The influence of media components on oxygen availability are also quantified for plant culture media. Experimental studies of polystyrene beads in suspension in a 30-litre air-lift and stirred bioreactors are used to illustrate design principles for circulation and mixing. Potential limitations to the use of liquid suspension culture due to plant physiological requirements are acknowledged.
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References
Altman PL & Dittmer DS (1968) Metabolism, Biological Handbooks, Federation of American Societies for Experimental Biology, Bethesda Maryland
Asplund PA & Curtis WR (2001) Intrinsic oxygen use kinetics of transformed root culture, Biotechnology Progress, 17:481–489
Blanch HW & Clark DS (1997) Biochemical Engineering, Marcel Dekker, New York
Curtis WR (1999) Achieving economic feasibility for moderate-value food and flavor additives: A perspective on productivity and proposal for production technology cost reduction, In: Fu T J; Singh G; Curtis W R (eds) Plant Cell and Tissue Culture for the Production of Food Ingredients (pp. 225–236) Kluwer Academic / Plenum Publishing, New York, NY
Curtis WR (2001) Method and apparatus for aseptic growth or processing of biomass, U.S. Patent # 6,245,555, June 12
Curtis WR & Emery AH (1993) Plant cell suspension culture rheology, Biotechnology and Bioengineering, 42:520–526
Gamborg OL, Miller RA & Ojima K (1968). Nutrient requirements of suspension cultures of soybean root cells, Exp. Cell Res., 50:151–158
Gupta PK & Durzan DJ (1985) Shoot multiplication from mature trees of Douglas-fir (Pseudotsuga menziesii) and sugar pine (Pinus lambertiana), Plant Cell Reports: 177–179
Hsiao TY, Bacani FT, Carvalho EB & Curtis WR (1999) Development of a low capital investment reactor system: Application for plant cell suspension culture, Biotechnology Progress, 15(1):114–122
Murashige T & Skoog F (1962) A revised medium for rapid growth and bio assays with tobacco tissue cultures, Physiol. Plant. 15: 473–497
Ramakrishnan D & Curtis WR (1995) Elevated meristematic respiration in plant root cultures: implications to reactor design, Journal of Chemical Engineering of Japan, 28(4):491–493
Schumpe A, Quicker G & Deckwer WD (1981) Gas solubilities in microbial culture media, In: Feichter A (ed) Advances in Biochemical Engineering, Vol. 24 (pp. 1–38). Springer-Verlag, New York, NY
Singh G & Curtis WR (1994) Reactor design for plant cell suspension culture, In: Shargool P D & Ngo T T (eds) Biotechnological Applications of Plant Culture (pp. 153–184). CRC Press, Boca Raton, FL
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© 2005 Springer
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Curtis, W.R. (2005). Application of bioreactor design principles to plant micropropagation. In: Hvoslef-Eide, A.K., Preil, W. (eds) Liquid Culture Systems for in vitro Plant Propagation. Springer, Dordrecht. https://doi.org/10.1007/1-4020-3200-5_2
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DOI: https://doi.org/10.1007/1-4020-3200-5_2
Publisher Name: Springer, Dordrecht
Print ISBN: 978-1-4020-3199-1
Online ISBN: 978-1-4020-3200-4
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