5.4 Conclusions
We described in this chapter the main characteristics of a newmulti-electrode array system that make it of interest for the development of drug-testing approaches. As we showed here, this system is relatively simple and easy to handle; it is characterized by an excellent stability of recordings, capacity to easily run pharmacological and toxicological experiments by exchanging the perfusion medium and monitoring effects on synaptic transmission or tissue viability for several days or weeks, and the possibility to apply this approach to even more complex physiological models such as lesion-induced regeneration or tissue re-myelination.
In comparison to other or previous multi-electrode arrays, the main advantage of the Neurosensor Interface System is probably the design based on a low-cost, disposable cartridge and membrane array that renders its use simple, reliable, and compatible for simultaneous large-scale applications. In contrast, one disadvantage might be the relatively limited number of electrodes available for simultaneous recordings of activity and the difficulty to exploit the system in such a way as to perform single-cell spike recordings or unit recordings. The objective however, when designing this array was to privilege a development compatible with large-scale screening of compounds rather than analysis of signal integration or single-cell properties within a complex network.
The system has therefore been developed to ensure versatility and applicability to numerous different biological models susceptible to be of relevance for drug development.
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Keywords
- Slice Culture
- Hippocampal Slice Culture
- Organotypic Slice Culture
- Electrophysiological Monitoring
- Hippocampal Organotypic Slice Culture
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Hakkoum, D., Muller, D., Stoppini, L. (2006). Electrophysiological Monitoring of Hippocampal Slice Cultures Using MEA on Porous Membrane. In: Taketani, M., Baudry, M. (eds) Advances in Network Electrophysiology. Springer, Boston, MA . https://doi.org/10.1007/0-387-25858-2_5
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