Overview
- Editors:
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Konrad Sachse
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Federal Institute for Health Protection of Consumers and Veterinary Medicine (BgVV), Jena, Germany
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Joachim Frey
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University of Bern, Bern, Switzerland
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About this book
The polymerase chain reaction (PCR) has become an increasingly important tool in microbial diagnosis in recent years, mainly because of its rapidity, high sensitivity, and specificity. In a number of instances, parti- larly with noncultivatable and slow-growing microorganisms, reliable iden- fication and differentiation became possible only after the introduction of PCR-based methods. Because the number of published procedures is large and steadily rising, there is a need for critical evaluation, comparison of performance, and even- ally also standardization of methods to enable laboratory diagnosticians to select the optimal methodology. PCR Detection of Microbial Pathogens is designed to provide an ov- view of the possibilities and problems connected with the use of PCR-based identification and detection of important bacterial and other microbial pat- gens, among them several zoonotic agents. It is intended to provide micro- ologists and biochemists the opportunity to extend their knowledge of state-of-the-art detection procedures, as well as pre-PCR sample processing and various general aspects of PCR. The chapters of this volume have been written by investigators well conversant with molecular detection and iden- fication of microorganisms. They have contributed their extensive practical experience to the protocols, not only concerning PCR methodology itself, but also the highly important area of pre-amplification sample processing.
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Article
Open access
30 December 2014
Table of contents (22 protocols)
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Protocols
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- Henrik Christensen, Magne Bisgaard, Jesper Larsen, John Elmerdahl Olsen
Pages 257-274
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- Burkhard Malorny, Reiner Helmuth
Pages 275-287
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- Jonathan M. Wastling, Jens G. Mattsson
Pages 289-298
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- Edoardo Pozio, Giuseppe La Rosa
Pages 299-309
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- Rickard Knutsson, Peter Rådström
Pages 311-324
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Back Matter
Pages 325-334
Reviews
"...PCR Detection of Microbial Pathogens is useful, comprehensive and practical guide for everyone who wants to use PCR for detection of the above mentioned pathogens and, at the same time, an inspiration for development of new PCR detection systems." -Folia Microbiologica
Editors and Affiliations
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Federal Institute for Health Protection of Consumers and Veterinary Medicine (BgVV), Jena, Germany
Konrad Sachse
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University of Bern, Bern, Switzerland
Joachim Frey