Overview
- Editors:
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Ralph Rapley
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University of Hertfordshire, Hatfield, UK
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David L. Manning
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Tenovus Institute for Cancer Research, Cardiff, UK
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About this book
Ribonucleic acids are central to cellular and molecular processes and perform vital functions in both structural and functional roles. RNA molecules form the bridge between the stable genetic information contained within DNA and enzymes and proteins that carry out much of the metabolism within the cell. Many of the sites of protein synthesis, the ribosomes within the cell, are composed of these ribonucleic acids as are the tRNA molecules that deliver the amino acid building blocks to the ribosomes. Of all the RNA species, the nucleic acid intermediate, messenger RNA, is a desirable source of material to biologists, since this reflects much of, what ultimately, is translated into enzymes and proteins. In order to determine the qualitative and quantitative changes in mRNA expression, a vast number of molecular biological techniques have been developed. Key molecular methods that provide the means to initially isolate and analyze RNA molecules are the focus of this volume. In putting together this collection of protocols, we have tried to provide techniques that are most applicable and widely used. In particular, there are a number of iso- tion techniques included that have been developed, modified, or adapted to enable extraction from a variety of cell types, organisms, or subcellular organelles. Successful isolation of intact RNA is an essential starting point for any sub- quent analysis. This is why we have aimed to make this section comprehensive. The analysis of RNA is the focus of the following chapters.
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Article
Open access
15 March 2018
Table of contents (33 protocols)
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- Thomas C. G. Bosch, Jan U. Lohmann
Pages 153-160
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- François Mallet, Guy Oriol, Bernard Mandrand
Pages 161-172
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- Maggie Walmsley, Mark Leonard, Roger Patient
Pages 187-193
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- Stéphane Viville, Roberto Mantovani
Pages 195-200
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- Rai Ajit K. Srivastava, Gustav Schonfeld
Pages 201-207
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- Louise Olliver, Charles D. Boyd
Pages 221-227
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- Louise Olliver, Anne Grobler-Rabie, Charles D. Boyd
Pages 229-233
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- Glenn M. Matthews, Alan Colman
Pages 235-247
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- Lesley-Ann Martin, Peter P. C. Mertens
Pages 249-260
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Back Matter
Pages 261-264
Editors and Affiliations
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University of Hertfordshire, Hatfield, UK
Ralph Rapley
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Tenovus Institute for Cancer Research, Cardiff, UK
David L. Manning